ID   MURL_MICS3              Reviewed;         447 AA.
AC   C4RJF8;
DT   10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT   07-JUL-2009, sequence version 1.
DT   25-MAY-2022, entry version 28.
DE   RecName: Full=UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-glutamate epimerase {ECO:0000255|HAMAP-Rule:MF_02209, ECO:0000305};
DE            EC=5.1.1.23 {ECO:0000255|HAMAP-Rule:MF_02209, ECO:0000269|PubMed:28294606};
DE   AltName: Full=UDP-MurNAc-L-Ala-L-Glu epimerase {ECO:0000255|HAMAP-Rule:MF_02209, ECO:0000303|PubMed:28294606};
GN   Name=murL {ECO:0000255|HAMAP-Rule:MF_02209, ECO:0000303|PubMed:28294606};
GN   ORFNames=MCAG_01010 {ECO:0000312|EMBL:EEP70683.1};
OS   Micromonospora sp. (strain ATCC 39149 / NRRL 15099 / SCC 1413).
OC   Bacteria; Actinobacteria; Micromonosporales; Micromonosporaceae;
OC   Micromonospora; unclassified Micromonospora.
OX   NCBI_TaxID=219305;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39149 / NRRL 15099 / SCC 1413;
RG   The Broad Institute Genome Sequencing Platform;
RG   Broad Institute Microbial Sequencing Center;
RA   Fischbach M., Godfrey P., Ward D., Young S., Kodira C.D., Zeng Q.,
RA   Koehrsen M., Alvarado L., Berlin A.M., Borenstein D., Chen Z., Engels R.,
RA   Freedman E., Gellesch M., Goldberg J., Griggs A., Gujja S., Heiman D.I.,
RA   Hepburn T.A., Howarth C., Jen D., Larson L., Lewis B., Mehta T., Park D.,
RA   Pearson M., Roberts A., Saif S., Shea T.D., Shenoy N., Sisk P., Stolte C.,
RA   Sykes S.N., Walk T., White J., Yandava C., Straight P., Clardy J., Hung D.,
RA   Kolter R., Mekalanos J., Walker S., Walsh C.T., Wieland-Brown L.C.,
RA   Galagan J., Nusbaum C., Birren B.;
RT   "The genome sequence of Micromonospora carbonacea var. africana strain ATCC
RT   39149.";
RL   Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RC   STRAIN=ATCC 39149 / NRRL 15099 / SCC 1413;
RX   PubMed=28294606; DOI=10.1021/jacs.7b01221;
RA   Feng R., Satoh Y., Ogasawara Y., Yoshimura T., Dairi T.;
RT   "A glycopeptidyl-glutamate epimerase for bacterial peptidoglycan
RT   biosynthesis.";
RL   J. Am. Chem. Soc. 139:4243-4245(2017).
CC   -!- FUNCTION: Cell wall formation. Catalyzes epimerization of the terminal
CC       L-glutamate in UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-glutamate.
CC       {ECO:0000255|HAMAP-Rule:MF_02209, ECO:0000269|PubMed:28294606}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-glutamate
CC         = AMP + diphosphate + H(+) + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-
CC         D-glutamate; Xref=Rhea:RHEA:58812, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:83900, ChEBI:CHEBI:142725, ChEBI:CHEBI:456215;
CC         EC=5.1.1.23; Evidence={ECO:0000255|HAMAP-Rule:MF_02209,
CC         ECO:0000269|PubMed:28294606};
CC   -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_02209, ECO:0000305|PubMed:28294606}.
CC   -!- SIMILARITY: Belongs to the MurL family. {ECO:0000255|HAMAP-
CC       Rule:MF_02209, ECO:0000305}.
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DR   EMBL; GG657738; EEP70683.1; -; Genomic_DNA.
DR   RefSeq; WP_007071959.1; NZ_GG657738.1.
DR   AlphaFoldDB; C4RJF8; -.
DR   STRING; 219305.MCAG_01010; -.
DR   EnsemblBacteria; EEP70683; EEP70683; MCAG_01010.
DR   eggNOG; COG1365; Bacteria.
DR   HOGENOM; CLU_045660_0_0_11; -.
DR   OMA; CRFVFLA; -.
DR   UniPathway; UPA00219; -.
DR   Proteomes; UP000010307; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR   GO; GO:0016855; F:racemase and epimerase activity, acting on amino acids and derivatives; IEA:UniProtKB-UniRule.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   HAMAP; MF_02209; MurL; 1.
DR   InterPro; IPR043689; MurL.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Cell shape; Cell wall biogenesis/degradation;
KW   Isomerase; Peptidoglycan synthesis; Reference proteome.
FT   CHAIN           1..447
FT                   /note="UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-glutamate
FT                   epimerase"
FT                   /id="PRO_0000446511"
SQ   SEQUENCE   447 AA;  48271 MW;  3E54223794070959 CRC64;
     MPNEQLRRMD AFTFPSYSID LATGEALFDY ALTGPDGEQR FTEVITLPLP AEPPSDATVA
     TLGRVLELLH VVAGVSYYKA AAPRRLVLPA PLGEAAAALV TAVYTKGLAE YAYRNQLPHV
     LELTPEIPAG SVPPAREYDN SDLRPLSAVG GGKDSIVSLE ALRRAGLDPV PFSVNPNHVI
     VAVNEASGLA PLAARRRIDP VLFDLNAAGA RNGHIPVTAI NSLIAVATAV LNRLGPVVMS
     NERSASDPNL VWNGHEINHQ WSKGVEAEGL LRAALAEHAG LTEPYFSLLR SLSELHIARL
     FAQIDRYDDV VTSCNAAFKL RDASERWCRD CPKCRFVFLA MAPFMPRERV VHIFGGDLLA
     DETQIPGYRE LLGVDGHKPF ECVGEVEESV VALSLLAEQD QWRDAPVVRA LVDAVPETAW
     SAAATSDVFT PGGPHHIPPP YAKALAQ