ID   A12_VACCW               Reviewed;         192 AA.
AC   Q80HV7;
DT   07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   23-FEB-2022, entry version 45.
DE   RecName: Full=25 kDa core protein A12L;
DE   Contains:
DE     RecName: Full=17 kDa core protein A12L;
DE              Short=17K;
GN   OrderedLocusNames=VACWR131; ORFNames=A12L;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND MUTAGENESIS OF 55-ALA--ALA-57.
RX   PubMed=17625005; DOI=10.1186/1743-422x-4-73;
RA   Yang S.J., Hruby D.E.;
RT   "Vaccinia virus A12L protein and its AG/A proteolysis play an important
RT   role in viral morphogenic transition.";
RL   Virol. J. 4:73-73(2007).
RN   [3]
RP   PROTEOLYTIC PROCESSING, INDUCTION, AND MUTAGENESIS OF 55-ALA--ALA-57.
RX   PubMed=17678539; DOI=10.1186/1743-422x-4-78;
RA   Yang S.J.;
RT   "Characterization of vaccinia virus A12L protein proteolysis and its
RT   participation in virus assembly.";
RL   Virol. J. 4:78-78(2007).
CC   -!- FUNCTION: Component of the virion core that undergoes proteolytic
CC       processing during the immature virion (IV) to mature virion (MV)
CC       transition. Essential for the formation of a structurally normal core.
CC       {ECO:0000269|PubMed:17625005}.
CC   -!- SUBCELLULAR LOCATION: [25 kDa core protein A12L]: Virion.
CC       Note=Localizes to the virion core. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: [17 kDa core protein A12L]: Virion.
CC       Note=Localizes to the virion core. {ECO:0000305}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:17678539}.
CC   -!- PTM: The 25-kDa precursor is cleaved to a mature protein of 17 kDa
CC       during virion maturation. Further proteolytic processing is supposed to
CC       occur since five more A12L-derived products have been observed.
CC       {ECO:0000269|PubMed:17678539}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae A12 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY243312; AAO89410.1; -; Genomic_DNA.
DR   RefSeq; YP_233013.1; NC_006998.1.
DR   SMR; Q80HV7; -.
DR   DNASU; 3707529; -.
DR   GeneID; 3707529; -.
DR   KEGG; vg:3707529; -.
DR   Proteomes; UP000000344; Genome.
DR   InterPro; IPR006744; Poxvirus_A12.
DR   Pfam; PF04651; Pox_A12; 1.
PE   1: Evidence at protein level;
KW   Late protein; Reference proteome; Virion.
FT   CHAIN           1..192
FT                   /note="25 kDa core protein A12L"
FT                   /id="PRO_0000099237"
FT   CHAIN           1..56
FT                   /note="17 kDa core protein A12L"
FT                   /id="PRO_0000413915"
FT   REGION          69..104
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          152..178
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        164..178
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            56..57
FT                   /note="Cleavage; by I7 protease"
FT   MUTAGEN         55..57
FT                   /note="AGA->IDI: Aberrant virus particles."
FT                   /evidence="ECO:0000269|PubMed:17625005,
FT                   ECO:0000269|PubMed:17678539"
SQ   SEQUENCE   192 AA;  20489 MW;  C5966FCE1B806B86 CRC64;
     MADKKNLAVR SSYDDYIETV NKITPQLKNL LAQIGGDAAV KGGNNNLNSQ TDVTAGACDT
     KSKSSKCITC KPKSKSSSSS TSASKGSKNT SGAPRRRTTV TTTSYNAMDG QIVQAVTNAG
     KIVYGTVRDG QLEVRGMVGE INHDLLGIDS VNAGKKKPSK KMPTNKKINM SSGMRRQEQI
     NPDDCCLDMG MY