ID   MUS81_ASHGO             Reviewed;         604 AA.
AC   Q754C9;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=Crossover junction endonuclease MUS81;
DE            EC=3.1.22.-;
GN   Name=MUS81; OrderedLocusNames=AFR141C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Interacts with EME1 to form a DNA structure-specific
CC       endonuclease with substrate preference for branched DNA structures with
CC       a 5'-end at the branch nick. Typical substrates include 3'-flap
CC       structures, D-loops, replication forks and nicked Holliday junctions.
CC       May be required in mitosis for the processing of stalled or collapsed
CC       replication fork intermediates. May be required in meiosis for the
CC       repair of meiosis-specific double strand breaks subsequent to single-
CC       end invasion (SEI) (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Interacts with EME1. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the XPF family. {ECO:0000305}.
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DR   EMBL; AE016819; AAS53512.1; -; Genomic_DNA.
DR   RefSeq; NP_985688.1; NM_211042.1.
DR   AlphaFoldDB; Q754C9; -.
DR   SMR; Q754C9; -.
DR   STRING; 33169.AAS53512; -.
DR   EnsemblFungi; AAS53512; AAS53512; AGOS_AFR141C.
DR   GeneID; 4621939; -.
DR   KEGG; ago:AGOS_AFR141C; -.
DR   eggNOG; KOG2379; Eukaryota.
DR   HOGENOM; CLU_014329_1_0_1; -.
DR   InParanoid; Q754C9; -.
DR   OMA; WIKEWLD; -.
DR   Proteomes; UP000000591; Chromosome VI.
DR   GO; GO:0048476; C:Holliday junction resolvase complex; IBA:GO_Central.
DR   GO; GO:0043596; C:nuclear replication fork; IEA:EnsemblFungi.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0048257; F:3'-flap endonuclease activity; IBA:GO_Central.
DR   GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:EnsemblFungi.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0004857; F:enzyme inhibitor activity; IEA:EnsemblFungi.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000737; P:DNA catabolic process, endonucleolytic; IEA:InterPro.
DR   GO; GO:0006265; P:DNA topological change; IEA:EnsemblFungi.
DR   GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR   GO; GO:0031573; P:mitotic intra-S DNA damage checkpoint signaling; IBA:GO_Central.
DR   GO; GO:0051097; P:negative regulation of helicase activity; IEA:EnsemblFungi.
DR   GO; GO:0000712; P:resolution of meiotic recombination intermediates; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 1.10.150.110; -; 1.
DR   Gene3D; 1.10.150.670; -; 1.
DR   InterPro; IPR027421; DNA_pol_lamdba_lyase_dom_sf.
DR   InterPro; IPR042530; EME1/EME2_C.
DR   InterPro; IPR006166; ERCC4_domain.
DR   InterPro; IPR033309; Mus81.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR13451; PTHR13451; 1.
DR   Pfam; PF02732; ERCC4; 1.
DR   SMART; SM00891; ERCC4; 1.
DR   SUPFAM; SSF47802; SSF47802; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; Endonuclease; Hydrolase;
KW   Magnesium; Meiosis; Metal-binding; Nuclease; Nucleus; Reference proteome.
FT   CHAIN           1..604
FT                   /note="Crossover junction endonuclease MUS81"
FT                   /id="PRO_0000223639"
FT   DOMAIN          322..419
FT                   /note="ERCC4"
FT   REGION          268..290
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   604 AA;  68728 MW;  6FF39C38866706EB CRC64;
     MSAKQLGDLK PLFIEWLKDE IDACGPRQEK MAMVYFRARE SLKRFDQPIT ETAKVLKVKG
     IGNSIKNKLA SKLAQYCQDN NIPLEDAAAP SHVVGISVTR VRTATQEKDD QSPKRKKRKY
     VPRKRSGAYA ILLGALELGC PSRGLTKEEI IDAAAKYCDV SFVSNPLTRE FYSAWTAIKV
     LIDHDLMLEQ GRPRRYIVTE AGEQMAETLK HADSVIFPED CPYQRRQQAP ESTAEDHTEL
     SASLSELVRQ EHLPQNHSGM CFSFEPPSSA PSDYLDPGQK SANVASSPHR LRPPIGPSID
     IVKARWNGTS YELWKPGSYD IELYIDHREV RAKSERDFFV NALLTRGVTV EGKALALGDM
     VWVARHRDSR STCVLNFMLE RKRLDDLAMS IRDNRFMEQK NRLKKTGCKH IFYLVEETSG
     TNVAGMEGAI KTSIWMTYVY SGFHVKRTRN ADDTVEWLHD MTCTVQRYYC SKSLLVLRPR
     EIANQEDYGS LLSAFRLQFE RNNTSLECCH AFDCYQEVLG KTGLMTVKEL YIRTLMLNRG
     VSLEKALAIQ SKFPTLRSLM TAFRNCKSEE DGRRMLYLAL LDQPASRRVG KALAATLWDT
     FGRR