MUS81_CANAL
ID MUS81_CANAL Reviewed; 614 AA.
AC Q59NG5; A0A1D8PPF2;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Crossover junction endonuclease MUS81;
DE EC=3.1.22.-;
GN Name=MUS81; OrderedLocusNames=CAALFM_C600540WA;
GN ORFNames=CaO19.11682, CaO19.4206;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC -!- FUNCTION: Interacts with EME1 to form a DNA structure-specific
CC endonuclease with substrate preference for branched DNA structures with
CC a 5'-end at the branch nick. Typical substrates include 3'-flap
CC structures, D-loops, replication forks and nicked Holliday junctions.
CC May be required in mitosis for the processing of stalled or collapsed
CC replication fork intermediates. May be required in meiosis for the
CC repair of meiosis-specific double strand breaks subsequent to single-
CC end invasion (SEI) (By similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SUBUNIT: Interacts with EME1. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the XPF family. {ECO:0000305}.
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DR EMBL; CP017628; AOW30002.1; -; Genomic_DNA.
DR RefSeq; XP_711258.1; XM_706166.1.
DR AlphaFoldDB; Q59NG5; -.
DR SMR; Q59NG5; -.
DR STRING; 237561.Q59NG5; -.
DR PRIDE; Q59NG5; -.
DR GeneID; 3647146; -.
DR KEGG; cal:CAALFM_C600540WA; -.
DR CGD; CAL0000174024; orf19.11682.
DR VEuPathDB; FungiDB:C6_00540W_A; -.
DR HOGENOM; CLU_014329_1_0_1; -.
DR InParanoid; Q59NG5; -.
DR OrthoDB; 738810at2759; -.
DR PRO; PR:Q59NG5; -.
DR Proteomes; UP000000559; Chromosome 6.
DR GO; GO:0048476; C:Holliday junction resolvase complex; IBA:GO_Central.
DR GO; GO:0043596; C:nuclear replication fork; IEA:EnsemblFungi.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0048257; F:3'-flap endonuclease activity; IBA:GO_Central.
DR GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:EnsemblFungi.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004857; F:enzyme inhibitor activity; IEA:EnsemblFungi.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000737; P:DNA catabolic process, endonucleolytic; IEA:InterPro.
DR GO; GO:0006265; P:DNA topological change; IEA:EnsemblFungi.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR GO; GO:0044182; P:filamentous growth of a population of unicellular organisms; IEA:UniProt.
DR GO; GO:0031573; P:mitotic intra-S DNA damage checkpoint signaling; IBA:GO_Central.
DR GO; GO:0051097; P:negative regulation of helicase activity; IEA:EnsemblFungi.
DR GO; GO:0000712; P:resolution of meiotic recombination intermediates; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.150.110; -; 1.
DR Gene3D; 1.10.150.670; -; 1.
DR InterPro; IPR027421; DNA_pol_lamdba_lyase_dom_sf.
DR InterPro; IPR042530; EME1/EME2_C.
DR InterPro; IPR006166; ERCC4_domain.
DR InterPro; IPR033309; Mus81.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR13451; PTHR13451; 1.
DR Pfam; PF02732; ERCC4; 1.
DR SMART; SM00891; ERCC4; 1.
DR SUPFAM; SSF47802; SSF47802; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA recombination; DNA repair; Endonuclease; Hydrolase;
KW Magnesium; Meiosis; Metal-binding; Nuclease; Nucleus; Reference proteome.
FT CHAIN 1..614
FT /note="Crossover junction endonuclease MUS81"
FT /id="PRO_0000223641"
FT DOMAIN 324..421
FT /note="ERCC4"
FT REGION 102..128
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 102..116
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 614 AA; 70663 MW; 11B248C3953A29A2 CRC64;
MSVVNDDFKP LLIQWVEEEA INATKRGSKM VDLYNKILFQ LRGYELPICD LKTLKSIKYV
GDKTANQLRK KIAKHCKENE LTLPRGFMDV AEKHRLDLEE SNLTSSTSTI KTTNKRAKTS
SKSKPKYVPK HRSGGFAILI ALYLYDKDRN GMTKERIIAG ATPYCDRSFS NNAASKEFYS
AWSSAKTLEN HNLISTTGRS PKIYFLTDEG VSLAQQLKTA IGLSSPPEGS KKNDRNMIME
QSFDNGIRLD TSFELSSPAG RAMLSSSPIR RISNPVHSNR AIQKMLEKEL RNGETPPSSQ
HDAGNRIYDG IKYEVWRKED YEVIVYMDNR EIRSRADRDH FQTRLQTLGV KCEVKPLSSG
DVLWVGRNTS TGTEAVLNYL CERKRLDDLC DSIKDGRFQE QKNRMKKTGI KHCYYLVEDM
VSYQDKVYDL MDSIQSSLTQ TMTTARLYLR RFKDIDETTA FIASNTKVIE NLKSNLIVIK
PQDIKNQQDY LNILLKFRNK FEKTQPHQDN PDYECVQLFS RFQDMLGKTN QMTVKEMFIL
MLMTIRGVSL EKAVVIQNRF PTPKSLLEYY HTEHATTDTN IKRDLMMNEF KDQIGNKKIG
KALSEKIYNV WGKP
