MUS81_NEUCR
ID MUS81_NEUCR Reviewed; 645 AA.
AC Q7SD49;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2003, sequence version 1.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=Crossover junction endonuclease mus-81;
DE EC=3.1.22.-;
GN Name=mus-81; ORFNames=NCU07457;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- FUNCTION: Interacts with eme-1 to form a DNA structure-specific
CC endonuclease with substrate preference for branched DNA structures with
CC a 5'-end at the branch nick. Typical substrates include 3'-flap
CC structures, D-loops, replication forks and nicked Holliday junctions.
CC May be required in mitosis for the processing of stalled or collapsed
CC replication fork intermediates. May be required in meiosis for the
CC repair of meiosis-specific double strand breaks subsequent to single-
CC end invasion (SEI) (By similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SUBUNIT: Interacts with eme-1. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the XPF family. {ECO:0000305}.
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DR EMBL; CM002236; EAA34672.1; -; Genomic_DNA.
DR RefSeq; XP_963908.1; XM_958815.2.
DR AlphaFoldDB; Q7SD49; -.
DR SMR; Q7SD49; -.
DR STRING; 5141.EFNCRP00000007371; -.
DR EnsemblFungi; EAA34672; EAA34672; NCU07457.
DR GeneID; 3880057; -.
DR KEGG; ncr:NCU07457; -.
DR VEuPathDB; FungiDB:NCU07457; -.
DR HOGENOM; CLU_014329_1_0_1; -.
DR InParanoid; Q7SD49; -.
DR OMA; WIKEWLD; -.
DR Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR GO; GO:0048476; C:Holliday junction resolvase complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0048257; F:3'-flap endonuclease activity; IBA:GO_Central.
DR GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000737; P:DNA catabolic process, endonucleolytic; IEA:InterPro.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR GO; GO:0031573; P:mitotic intra-S DNA damage checkpoint signaling; IBA:GO_Central.
DR GO; GO:0000712; P:resolution of meiotic recombination intermediates; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.150.110; -; 1.
DR Gene3D; 1.10.150.670; -; 1.
DR InterPro; IPR027421; DNA_pol_lamdba_lyase_dom_sf.
DR InterPro; IPR042530; EME1/EME2_C.
DR InterPro; IPR006166; ERCC4_domain.
DR InterPro; IPR033309; Mus81.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR13451; PTHR13451; 1.
DR Pfam; PF02732; ERCC4; 1.
DR SMART; SM00891; ERCC4; 1.
DR SUPFAM; SSF47802; SSF47802; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA recombination; DNA repair; Endonuclease; Hydrolase;
KW Magnesium; Meiosis; Metal-binding; Nuclease; Nucleus; Reference proteome.
FT CHAIN 1..645
FT /note="Crossover junction endonuclease mus-81"
FT /id="PRO_0000223646"
FT DOMAIN 353..459
FT /note="ERCC4"
FT REGION 98..119
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 219..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 219..250
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 645 AA; 73224 MW; 74B61CCC43E83788 CRC64;
MAGDTGANPL FLGWVKEWWD TAREHNTKGA PTYKKAYNSL KACPLTFQHP SELQVLNGFG
PTISQRLTDR LKQYCEENGL PMPKHPKRKR TLELESALAA AGAVQDEQPP PPKRARTARP
YVPKLNSGAY ALLMALSELG PKEFMDKTTL IAKAQPYSEH SFTVPTMANK SYTAWDSMKT
LEQKELVWLR GLPSKRYSLT DEGWEVVKRM KEAQNLVDGV AGSANTSRNA IASGSGTSNP
NRSENVNPNR QDSGVKRESR YTPLDLKPFV TTTATPERPL QPKPHTANSE YSIIIDSDDE
DPKYDEEDRK PIIRDTTNRD YIDLVADGDS VPDESNLPHF TPIRLAPGSF TVELVLDTRE
VQAKNNRDHI QEELSKLGVR PVMRSLELGD VLWIAKCKQP GWLNRLGAEG DEVVLDYIVE
RKRLDDLIGS IKDGRFREQK YRLKRSGMKN VVYIIENYNI DMDIRRQYQD AMDTAMASIQ
VVNGYFLKKT DTIAESIRYL AAVTYMLKEI YESKPLFVIP TQVLTAKNYL PLVKHLREKE
PSRGYYISYP AFASLVSKSE MMTLKDVFIK MLMCIRRLSG EKAIEIQKVW KTPYQLVKAF
EACGSDENGK KKQKVLVMSM LSHLVDRRNV DKGLSERIAD VWGFL
