MUS81_RAT
ID MUS81_RAT Reviewed; 551 AA.
AC Q4KM32;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 02-AUG-2005, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Crossover junction endonuclease MUS81;
DE EC=3.1.22.-;
GN Name=Mus81;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Thymus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Interacts with EME1 and EME2 to form a DNA structure-specific
CC endonuclease with substrate preference for branched DNA structures with
CC a 5'-end at the branch nick. Typical substrates include 3'-flap
CC structures, replication forks and nicked Holliday junctions. May be
CC required in mitosis for the processing of stalled or collapsed
CC replication forks. {ECO:0000250|UniProtKB:Q96NY9}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- SUBUNIT: May self-associate. Interacts with EME1, EME2 and CHEK2.
CC Interacts with BLM, and this interaction may stimulate the endonuclease
CC activity of MUS81. Interacts with SLX4/BTBD12; this interaction is
CC direct and links the MUS81-EME1 complex to SLX4, which may coordinate
CC the action of the structure-specific endonuclease during DNA repair.
CC Interacts with DCLRE1B/Apollo. Interacts with RECQL5; this interaction
CC stimulates mitotic DNA synthesis (By similarity).
CC {ECO:0000250|UniProtKB:Q96NY9}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus. Note=Recruited to foci of DNA
CC damage in S-phase cells. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the XPF family. {ECO:0000305}.
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DR EMBL; BC098853; AAH98853.1; -; mRNA.
DR RefSeq; NP_001020816.1; NM_001025645.1.
DR AlphaFoldDB; Q4KM32; -.
DR SMR; Q4KM32; -.
DR STRING; 10116.ENSRNOP00000028015; -.
DR PaxDb; Q4KM32; -.
DR PRIDE; Q4KM32; -.
DR GeneID; 293678; -.
DR KEGG; rno:293678; -.
DR UCSC; RGD:1311957; rat.
DR CTD; 80198; -.
DR RGD; 1311957; Mus81.
DR VEuPathDB; HostDB:ENSRNOG00000020617; -.
DR eggNOG; KOG2379; Eukaryota.
DR HOGENOM; CLU_014329_3_0_1; -.
DR InParanoid; Q4KM32; -.
DR OMA; WIKEWLD; -.
DR OrthoDB; 738810at2759; -.
DR PhylomeDB; Q4KM32; -.
DR TreeFam; TF315113; -.
DR Reactome; R-RNO-5693568; Resolution of D-loop Structures through Holliday Junction Intermediates.
DR Reactome; R-RNO-6783310; Fanconi Anemia Pathway.
DR PRO; PR:Q4KM32; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000020617; Expressed in testis and 19 other tissues.
DR Genevisible; Q4KM32; RN.
DR GO; GO:1905347; C:endodeoxyribonuclease complex; ISO:RGD.
DR GO; GO:0048476; C:Holliday junction resolvase complex; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0048257; F:3'-flap endonuclease activity; ISO:RGD.
DR GO; GO:0008821; F:crossover junction endodeoxyribonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000737; P:DNA catabolic process, endonucleolytic; ISO:RGD.
DR GO; GO:0006281; P:DNA repair; ISO:RGD.
DR GO; GO:0006302; P:double-strand break repair; ISO:RGD.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR GO; GO:0031573; P:mitotic intra-S DNA damage checkpoint signaling; IBA:GO_Central.
DR GO; GO:0033687; P:osteoblast proliferation; ISO:RGD.
DR GO; GO:0031297; P:replication fork processing; ISO:RGD.
DR GO; GO:0000712; P:resolution of meiotic recombination intermediates; IBA:GO_Central.
DR GO; GO:0072429; P:response to intra-S DNA damage checkpoint signaling; ISO:RGD.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.150.110; -; 1.
DR Gene3D; 1.10.150.670; -; 1.
DR InterPro; IPR027421; DNA_pol_lamdba_lyase_dom_sf.
DR InterPro; IPR042530; EME1/EME2_C.
DR InterPro; IPR006166; ERCC4_domain.
DR InterPro; IPR033309; Mus81.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR13451; PTHR13451; 2.
DR Pfam; PF02732; ERCC4; 1.
DR SMART; SM00891; ERCC4; 1.
DR SUPFAM; SSF47802; SSF47802; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
PE 2: Evidence at transcript level;
KW DNA damage; DNA recombination; DNA repair; Endonuclease; Hydrolase;
KW Magnesium; Metal-binding; Nuclease; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..551
FT /note="Crossover junction endonuclease MUS81"
FT /id="PRO_0000223636"
FT DOMAIN 270..372
FT /note="ERCC4"
FT REGION 85..131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 125..244
FT /note="Interaction with BLM"
FT /evidence="ECO:0000250"
FT REGION 231..255
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 107..131
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 95
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96NY9"
SQ SEQUENCE 551 AA; 61903 MW; 3F42CC6A4E6559C1 CRC64;
MAEPVRLGRK RPLPVCPNPL FVRWLTEWRD EAASRGRHTR FVFQKALRSL QRYPLPLRNG
KEAKILQHFG DRLCRMLDER LKEHLASGGD HAPSSPSGKK RASKGPPAQV QGSSMPVPTQ
PQAGSTNAGY WPAQNSGARE ILLQLYREHL NSDGHSFLTK EELLQKCAQK TPRVVPESSR
PWPALRGLLH RNLVLRTHRP ARYALTPEGL ELAQKLAEAE GLSTLNTAFQ PEEHHEESPV
PEAILSEPGT TEVGVQQRPL ELRPSEYRVL LCVDIGETRG AGHRPEMLRE LQRLRVPHTV
RKLHVGDFVW VAQETRPRDP ERPGELVLDH IVERKRLDDL CSSIIDGRFR EQKFRLKRCG
LGHRIYLVEE HGSVQNLSLP ESTLLQAVTN TQVIDGFFVK RTMDIKESAG YLALLTKGLE
RLYQGHTLHS RPWGTPGDAE SEAKPSTNPL CSLLTFSDFN AEAVKNKAQS VREVFARQLM
QVRGLSGEKA AALVDRYSTP ASLLAAYDAC ATTKEQEMLL STVKCGRLQR NLGPALSRTL
YQLYCSHSPL T
