MUSC_MOUSE
ID MUSC_MOUSE Reviewed; 201 AA.
AC O88940; Q3ZAZ1;
DT 14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Musculin;
DE AltName: Full=Myogenic repressor;
GN Name=Msc; Synonyms=Myor;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryonic skeletal muscle;
RX PubMed=9767165; DOI=10.1016/s0925-4773(98)00122-1;
RA Robb L., Hartley L., Wang C.-C., Harvey R.P., Begley C.G.;
RT "Musculin: a murine basic helix-loop-helix transcription factor gene
RT expressed in embryonic skeletal muscle.";
RL Mech. Dev. 76:197-201(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND MUTAGENESIS.
RX PubMed=9892671; DOI=10.1073/pnas.96.2.552;
RA Lu J.-R., Webb R., Richardson J.A., Olson E.N.;
RT "MyoR: a muscle-restricted basic helix-loop-helix transcription factor that
RT antagonizes the actions of MyoD.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:552-557(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Transcription repressor that blocks myogenesis and activation
CC of E-box dependent muscle genes.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. Binds DNA as a homodimer or a heterodimer. Forms a heterodimer
CC with TCF3.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DEVELOPMENTAL STAGE: Expression in embryos is largely restricted to
CC embryonic skeletal muscle lineage. First detected at 9.5 dpc in cells
CC within the first and second branchial arches. At 10.5 dpc, expression
CC was also observed in the myotomal compartment of rostral somites and by
CC 11.5 dpc in myotomes along the whole anterio-posterior axis, as well as
CC in cells within the developing forelimbs and hindlimbs. At 12.5-14.5
CC dpc, expression was confined to the skeletal muscle lineage (head,
CC neck, trunk, limbs and diaphragm but not cardiac and smooth muscle). At
CC 15 dpc, a peak expression was seen in neonatal limbs.
CC {ECO:0000269|PubMed:9767165}.
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DR EMBL; AF087035; AAC69869.1; -; mRNA.
DR EMBL; AF108216; AAD10053.1; -; mRNA.
DR EMBL; BC103592; AAI03593.1; -; mRNA.
DR EMBL; BC103593; AAI03594.1; -; mRNA.
DR EMBL; BC103594; AAI03595.1; -; mRNA.
DR EMBL; BC103623; AAI03624.1; -; mRNA.
DR CCDS; CCDS14825.1; -.
DR RefSeq; NP_034957.1; NM_010827.2.
DR AlphaFoldDB; O88940; -.
DR SMR; O88940; -.
DR STRING; 10090.ENSMUSP00000027062; -.
DR PhosphoSitePlus; O88940; -.
DR PaxDb; O88940; -.
DR PRIDE; O88940; -.
DR Antibodypedia; 25121; 249 antibodies from 30 providers.
DR DNASU; 17681; -.
DR Ensembl; ENSMUST00000027062; ENSMUSP00000027062; ENSMUSG00000025930.
DR GeneID; 17681; -.
DR KEGG; mmu:17681; -.
DR UCSC; uc007aja.2; mouse.
DR CTD; 9242; -.
DR MGI; MGI:1333884; Msc.
DR VEuPathDB; HostDB:ENSMUSG00000025930; -.
DR eggNOG; KOG4029; Eukaryota.
DR GeneTree; ENSGT00940000160438; -.
DR HOGENOM; CLU_092663_1_0_1; -.
DR InParanoid; O88940; -.
DR OMA; GGCKKKR; -.
DR OrthoDB; 1374128at2759; -.
DR PhylomeDB; O88940; -.
DR TreeFam; TF350742; -.
DR BioGRID-ORCS; 17681; 2 hits in 74 CRISPR screens.
DR ChiTaRS; Msc; mouse.
DR PRO; PR:O88940; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; O88940; protein.
DR Bgee; ENSMUSG00000025930; Expressed in respiratory primordium and 102 other tissues.
DR ExpressionAtlas; O88940; baseline and differential.
DR Genevisible; O88940; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0014707; P:branchiomeric skeletal muscle development; IGI:UniProtKB.
DR GO; GO:1990830; P:cellular response to leukemia inhibitory factor; IEP:MGI.
DR GO; GO:0032502; P:developmental process; IBA:GO_Central.
DR GO; GO:0060539; P:diaphragm development; IGI:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0060021; P:roof of mouth development; IGI:UniProtKB.
DR GO; GO:0007519; P:skeletal muscle tissue development; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..201
FT /note="Musculin"
FT /id="PRO_0000127284"
FT DOMAIN 102..154
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 182..201
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 66..71
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT MUTAGEN 110..112
FT /note="RER->LEG: Loss of DNA-binding."
FT /evidence="ECO:0000269|PubMed:9892671"
SQ SEQUENCE 201 AA; 21538 MW; 04C757F710C69E32 CRC64;
MSTGSVSDPE DSEMRGLQRV YPAPASKRPP LLRMERGYGS PSDISSAEEE DGEEEPGSLG
AAGGCKRKRL RGADAGGAGG RAGGAGKKPL PPKGSAAECK QSQRNAANAR ERARMRVLSK
AFSRLKTSLP WVPPDTKLSK LDTLRLASSY IAHLRQLLQE DRYEDSYVHP VNLTWPFVVS
GRPDSDSKDV SAANRLCGTS A
