MUT7_HUMAN
ID MUT7_HUMAN Reviewed; 876 AA.
AC Q8N9H8; A0A140VJP9; B8A4U7; C9JIQ9; E9PN82; Q2TAK4; Q6P1M1; Q8IXT8; Q8N3X4;
AC Q8NHD1; Q96NP1; Q9NX53;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 3.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Exonuclease mut-7 homolog;
DE EC=3.1.-.-;
DE AltName: Full=Exonuclease 3'-5' domain-containing protein 3;
GN Name=EXD3; ORFNames=HBE269;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
RA Hasegawa H., Yonezawa T., Ninomiya Y.;
RT "A novel gene from human brain endothelial cell.";
RL Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Li J.Y.;
RT "Human testis protein.";
RL Submitted (MAR-2010) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 5), AND VARIANT
RP GLN-220.
RC TISSUE=Cerebellum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 4 AND 5), AND VARIANT
RP GLN-20.
RC TISSUE=Lung, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Possesses 3'-5' exoribonuclease activity. Required for 3'-end
CC trimming of AGO1-bound miRNAs (By similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- INTERACTION:
CC Q8N9H8; Q15038: DAZAP2; NbExp=3; IntAct=EBI-750745, EBI-724310;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1;
CC IsoId=Q8N9H8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8N9H8-2; Sequence=VSP_031345, VSP_031348;
CC Name=3;
CC IsoId=Q8N9H8-3; Sequence=VSP_031344, VSP_031349, VSP_031350;
CC Name=4;
CC IsoId=Q8N9H8-4; Sequence=VSP_031346, VSP_031347;
CC Name=5;
CC IsoId=Q8N9H8-5; Sequence=VSP_059361, VSP_059362;
CC -!- SIMILARITY: Belongs to the mut-7 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH39252.2; Type=Erroneous translation; Note=Wrong choice of CDS.; Evidence={ECO:0000305};
CC Sequence=AAH65002.1; Type=Erroneous translation; Note=Wrong choice of CDS.; Evidence={ECO:0000305};
CC Sequence=BAA91167.1; Type=Erroneous translation; Note=Wrong choice of CDS.; Evidence={ECO:0000305};
CC Sequence=BAC04356.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
CC Sequence=EAW88381.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB061798; BAC10986.1; -; mRNA.
DR EMBL; HM005438; AEE61036.1; -; mRNA.
DR EMBL; AK000440; BAA91167.1; ALT_SEQ; mRNA.
DR EMBL; AK055004; BAB70838.1; -; mRNA.
DR EMBL; AK094438; BAC04356.1; ALT_SEQ; mRNA.
DR EMBL; BX322799; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471090; EAW88381.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BC037355; AAH37355.1; -; mRNA.
DR EMBL; BC039252; AAH39252.2; ALT_SEQ; mRNA.
DR EMBL; BC065002; AAH65002.1; ALT_SEQ; mRNA.
DR EMBL; BC110879; AAI10880.1; -; mRNA.
DR CCDS; CCDS48066.1; -. [Q8N9H8-1]
DR CCDS; CCDS75942.1; -. [Q8N9H8-4]
DR RefSeq; NP_001273752.1; NM_001286823.1. [Q8N9H8-4]
DR RefSeq; NP_060290.3; NM_017820.4. [Q8N9H8-1]
DR AlphaFoldDB; Q8N9H8; -.
DR SMR; Q8N9H8; -.
DR BioGRID; 120272; 23.
DR IntAct; Q8N9H8; 3.
DR STRING; 9606.ENSP00000340474; -.
DR iPTMnet; Q8N9H8; -.
DR PhosphoSitePlus; Q8N9H8; -.
DR BioMuta; EXD3; -.
DR DMDM; 308153580; -.
DR MassIVE; Q8N9H8; -.
DR PaxDb; Q8N9H8; -.
DR PeptideAtlas; Q8N9H8; -.
DR PRIDE; Q8N9H8; -.
DR ProteomicsDB; 72535; -. [Q8N9H8-1]
DR ProteomicsDB; 72536; -. [Q8N9H8-2]
DR ProteomicsDB; 72537; -. [Q8N9H8-3]
DR ProteomicsDB; 72538; -. [Q8N9H8-4]
DR Antibodypedia; 32432; 78 antibodies from 17 providers.
DR DNASU; 54932; -.
DR Ensembl; ENST00000340951.9; ENSP00000340474.4; ENSG00000187609.16. [Q8N9H8-1]
DR Ensembl; ENST00000465160.2; ENSP00000432895.1; ENSG00000187609.16. [Q8N9H8-5]
DR Ensembl; ENST00000479452.5; ENSP00000431859.1; ENSG00000187609.16. [Q8N9H8-4]
DR GeneID; 54932; -.
DR KEGG; hsa:54932; -.
DR MANE-Select; ENST00000340951.9; ENSP00000340474.4; NM_017820.5; NP_060290.3.
DR UCSC; uc004cmp.4; human. [Q8N9H8-1]
DR CTD; 54932; -.
DR DisGeNET; 54932; -.
DR GeneCards; EXD3; -.
DR HGNC; HGNC:26023; EXD3.
DR HPA; ENSG00000187609; Low tissue specificity.
DR neXtProt; NX_Q8N9H8; -.
DR OpenTargets; ENSG00000187609; -.
DR PharmGKB; PA164719422; -.
DR VEuPathDB; HostDB:ENSG00000187609; -.
DR eggNOG; KOG2207; Eukaryota.
DR GeneTree; ENSGT00390000006843; -.
DR HOGENOM; CLU_007046_0_0_1; -.
DR InParanoid; Q8N9H8; -.
DR OMA; CSNWANR; -.
DR OrthoDB; 1295758at2759; -.
DR PhylomeDB; Q8N9H8; -.
DR TreeFam; TF328546; -.
DR PathwayCommons; Q8N9H8; -.
DR SignaLink; Q8N9H8; -.
DR BioGRID-ORCS; 54932; 18 hits in 1078 CRISPR screens.
DR ChiTaRS; EXD3; human.
DR GenomeRNAi; 54932; -.
DR Pharos; Q8N9H8; Tbio.
DR PRO; PR:Q8N9H8; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q8N9H8; protein.
DR Bgee; ENSG00000187609; Expressed in right uterine tube and 122 other tissues.
DR ExpressionAtlas; Q8N9H8; baseline and differential.
DR Genevisible; Q8N9H8; HS.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR CDD; cd06146; mut-7_like_exo; 1.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR037432; Mut-7_DEDDy_dom.
DR InterPro; IPR002782; Mut7-C_RNAse_dom.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR Pfam; PF01927; Mut7-C; 1.
DR SMART; SM00474; 35EXOc; 1.
DR SUPFAM; SSF53098; SSF53098; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Exonuclease; Hydrolase; Magnesium; Metal-binding;
KW Nuclease; Reference proteome.
FT CHAIN 1..876
FT /note="Exonuclease mut-7 homolog"
FT /id="PRO_0000319059"
FT DOMAIN 517..571
FT /note="3'-5' exonuclease"
FT REGION 578..607
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 751..781
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 751..771
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..320
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_031344"
FT VAR_SEQ 1..277
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_031345"
FT VAR_SEQ 20..56
FT /note="RDPLLLLQALQTLWSTRERKQLREEAWRGFAALDDPL -> THRLLQPLLHL
FT SRVGCFQSATPQLAGPLEQPRRPLEH (in isoform 5)"
FT /id="VSP_059361"
FT VAR_SEQ 57..876
FT /note="Missing (in isoform 5)"
FT /id="VSP_059362"
FT VAR_SEQ 219..246
FT /note="RRYPEVTSLSLEKLSPKALSRQVLRLQE -> SCHVTAAGAAASGEERLGLA
FT GAQPSSPE (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_031346"
FT VAR_SEQ 247..876
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_031347"
FT VAR_SEQ 278..289
FT /note="KSLSQENWTDHV -> MSPSPSSRPPSS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_031348"
FT VAR_SEQ 610..638
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_031349"
FT VAR_SEQ 760..772
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_031350"
FT VARIANT 20
FT /note="R -> Q (in dbSNP:rs13291830)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_062225"
FT VARIANT 220
FT /note="R -> Q (in dbSNP:rs7389423)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_038945"
FT CONFLICT 105
FT /note="Q -> R (in Ref. 1; BAC10986 and 3; BAB70838/
FT BAC04356)"
FT /evidence="ECO:0000305"
FT CONFLICT 160
FT /note="A -> T (in Ref. 3; BAC04356 and 6; AAH37355/
FT AAI10880)"
FT /evidence="ECO:0000305"
FT CONFLICT 249
FT /note="G -> S (in Ref. 3; BAC04356)"
FT /evidence="ECO:0000305"
FT CONFLICT 322
FT /note="E -> D (in Ref. 1; BAC10986 and 3; BAC04356/
FT BAB70838)"
FT /evidence="ECO:0000305"
FT CONFLICT 519
FT /note="S -> T (in Ref. 1; BAC10986)"
FT /evidence="ECO:0000305"
FT CONFLICT 545
FT /note="C -> Y (in Ref. 1; BAC10986 and 3; BAC04356/
FT BAB70838)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 876 AA; 96598 MW; A9033CFCDE2D1B0D CRC64;
MDPGDPAGDP AAGERHRMGR DPLLLLQALQ TLWSTRERKQ LREEAWRGFA ALDDPLAGLL
DMLESCRGQR GEGPSLAAWI SHQLQCWLQA QPCPSLAQHS LRLKQLQARA VKVLTESPPS
LAAPLASIFQ LQDADRSCLL AHVHRLHHEG RFREAATLGA TLKLQSELGV EKMSIPLLLQ
DKVALVERYV AGFPDLQRRL LVLMDSWCQP GFDIKDVARR YPEVTSLSLE KLSPKALSRQ
VLRLQERYGV APALCPNAAI QQRLAALRHL CHKRFVEKSL SQENWTDHVQ GLVGQSPWLQ
EQLSQLLVSH SDPVTAAQCA MELLLPEERL PAAVAVELRR FRLQGRATEA DSRLEVKDMK
DRYYQLPIPR ENVHLLASWE DLTRHEGALL QCHQVVGVDV EWTPVFVAGG RPRPSLLQVA
VEGHVFLLDV LALSQPPTGQ GAQAFSRLVA QLLSDPSITK LGYGMVGDLQ KLGTSCPALA
HVEKQILGGM DLLLVHRQMR VASVPAPAVD RARELRGLSL LVQQVLGTAL DKTQQLSNWD
RRPLCEEQVI YAAADAYCLL EVHQALCREP ARFHLSEDLA GSRRPRHRER PGARKPPGLQ
KASAPAAPRQ VPVAVAVSEG AAPQIPARAF RVVCDNMLQG LARSLRCLGV DARMLGNGED
HRRAAEVARQ EGRIILTSGQ PFHKLRAQVG AGRCLSVDCS LKAQQQAKAV LKHFNVRVTH
ADIFSRCQAC NCDQYLKVSR DMMKQLMWLS SHQEGPRSSG DEATQSQAVQ EPGPAPDAAP
EGCTYDRPCR WLQMADLRAE TPDMLADGTR LQLAGVPVGV LRTPGLRCFY CCTGCGKVFW
DGSHLGRVAT HFRDMLESAP SPCEPSPAPS PASSPF
