MUTH_ECOLI
ID MUTH_ECOLI Reviewed; 229 AA.
AC P06722; Q2MA06; Q9R2X2; Q9R3A8; Q9R411; Q9S6P5; Q9S6P6; Q9S6P7;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=DNA mismatch repair protein MutH {ECO:0000255|HAMAP-Rule:MF_00759};
DE AltName: Full=Methyl-directed mismatch repair protein {ECO:0000255|HAMAP-Rule:MF_00759};
GN Name=mutH {ECO:0000255|HAMAP-Rule:MF_00759}; Synonyms=mutR, prv;
GN OrderedLocusNames=b2831, JW2799;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-25, AND MUTANTS
RP TS7 AND TS28.
RC STRAIN=K12;
RX PubMed=3031619; DOI=10.1093/nar/15.7.3073;
RA Grafstrom R.H., Hoess R.H.;
RT "Nucleotide sequence of the Escherichia coli mutH gene.";
RL Nucleic Acids Res. 15:3073-3084(1987).
RN [2]
RP SEQUENCE REVISION TO 83; 130-131 AND 136.
RC STRAIN=K12;
RA Feng G., Tsui H.T., Winkler M.E., Loh T.A., Marinus M.G.;
RL Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 58-157.
RC STRAIN=ECOR 1, ECOR 10, ECOR 13, ECOR 17, ECOR 20, ECOR 23, ECOR 24,
RC ECOR 26, ECOR 34, ECOR 35, ECOR 37, ECOR 4, ECOR 40, ECOR 41, ECOR 45,
RC ECOR 47, ECOR 49, ECOR 51, ECOR 52, ECOR 57, ECOR 58, ECOR 59, ECOR 62,
RC ECOR 64, ECOR 67, ECOR 68, ECOR 70, and O2:HN / ECOR 50 / P97 / UPEC;
RA Denamur E.;
RL Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
RX PubMed=9482749; DOI=10.1093/emboj/17.5.1526;
RA Ban C., Yang W.;
RT "Structural basis for MutH activation in E.coli mismatch repair and
RT relationship of MutH to restriction endonucleases.";
RL EMBO J. 17:1526-1534(1998).
CC -!- FUNCTION: Sequence-specific endonuclease that cleaves unmethylated GATC
CC sequences. It is involved in DNA mismatch repair.
CC -!- INTERACTION:
CC P06722; P76217: astD; NbExp=2; IntAct=EBI-545170, EBI-545161;
CC P06722; P23367: mutL; NbExp=8; IntAct=EBI-545170, EBI-554913;
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the MutH family. {ECO:0000255|HAMAP-
CC Rule:MF_00759}.
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DR EMBL; Y00113; CAA68292.1; ALT_SEQ; Genomic_DNA.
DR EMBL; U16361; AAB59986.1; -; Genomic_DNA.
DR EMBL; U29581; AAB40478.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75870.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76900.1; -; Genomic_DNA.
DR EMBL; AJ005726; CAB41510.1; -; Genomic_DNA.
DR EMBL; AJ005727; CAB41511.1; -; Genomic_DNA.
DR EMBL; AJ005728; CAB41512.1; -; Genomic_DNA.
DR EMBL; AJ005729; CAB41513.1; -; Genomic_DNA.
DR EMBL; AJ005730; CAB41514.1; -; Genomic_DNA.
DR EMBL; AJ005731; CAB41515.1; -; Genomic_DNA.
DR EMBL; AJ005732; CAB41516.1; -; Genomic_DNA.
DR EMBL; AJ005733; CAB41517.1; -; Genomic_DNA.
DR EMBL; AJ005734; CAB41518.1; -; Genomic_DNA.
DR EMBL; AJ005735; CAB41519.1; -; Genomic_DNA.
DR EMBL; AJ005736; CAB41520.1; -; Genomic_DNA.
DR EMBL; AJ005737; CAB41521.1; -; Genomic_DNA.
DR EMBL; AJ005738; CAB41522.1; -; Genomic_DNA.
DR EMBL; AJ005739; CAB41523.1; -; Genomic_DNA.
DR EMBL; AJ005740; CAB41524.1; -; Genomic_DNA.
DR EMBL; AJ005743; CAB41669.1; -; Genomic_DNA.
DR EMBL; AJ005744; CAB41670.1; -; Genomic_DNA.
DR EMBL; AJ005745; CAB41671.1; -; Genomic_DNA.
DR EMBL; AJ005746; CAB41696.1; -; Genomic_DNA.
DR EMBL; AJ005747; CAB41672.1; -; Genomic_DNA.
DR EMBL; AJ005748; CAB41673.1; -; Genomic_DNA.
DR EMBL; AJ005749; CAB41674.1; -; Genomic_DNA.
DR EMBL; AJ005750; CAB41675.1; -; Genomic_DNA.
DR EMBL; AJ005751; CAB41676.1; -; Genomic_DNA.
DR EMBL; AJ005752; CAB41677.1; -; Genomic_DNA.
DR EMBL; AJ005753; CAB41678.1; -; Genomic_DNA.
DR EMBL; AJ005755; CAB41679.1; -; Genomic_DNA.
DR EMBL; AJ238722; CAB91077.1; -; Genomic_DNA.
DR EMBL; AJ238719; CAB91074.1; -; Genomic_DNA.
DR EMBL; AJ238720; CAB91075.1; -; Genomic_DNA.
DR EMBL; AJ238721; CAB91076.1; -; Genomic_DNA.
DR PIR; H65065; MVECMH.
DR RefSeq; NP_417308.1; NC_000913.3.
DR RefSeq; WP_000082201.1; NZ_SSUV01000026.1.
DR PDB; 1AZO; X-ray; 1.70 A; A=1-229.
DR PDB; 2AZO; X-ray; 2.30 A; A/B=1-229.
DR PDBsum; 1AZO; -.
DR PDBsum; 2AZO; -.
DR AlphaFoldDB; P06722; -.
DR SMR; P06722; -.
DR BioGRID; 4263275; 194.
DR BioGRID; 851627; 1.
DR ComplexPortal; CPX-5541; MutHLS methyl-directed mismatch repair complex.
DR DIP; DIP-10284N; -.
DR IntAct; P06722; 10.
DR STRING; 511145.b2831; -.
DR PaxDb; P06722; -.
DR PRIDE; P06722; -.
DR EnsemblBacteria; AAC75870; AAC75870; b2831.
DR EnsemblBacteria; BAE76900; BAE76900; BAE76900.
DR GeneID; 947299; -.
DR KEGG; ecj:JW2799; -.
DR KEGG; eco:b2831; -.
DR PATRIC; fig|1411691.4.peg.3903; -.
DR EchoBASE; EB0619; -.
DR eggNOG; COG3066; Bacteria.
DR HOGENOM; CLU_086669_0_0_6; -.
DR OMA; EVMQLRP; -.
DR PhylomeDB; P06722; -.
DR BioCyc; EcoCyc:EG10624-MON; -.
DR BioCyc; MetaCyc:EG10624-MON; -.
DR EvolutionaryTrace; P06722; -.
DR PRO; PR:P06722; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0032300; C:mismatch repair complex; IC:ComplexPortal.
DR GO; GO:0003677; F:DNA binding; IDA:EcoliWiki.
DR GO; GO:0004519; F:endonuclease activity; IDA:EcoliWiki.
DR GO; GO:0043765; F:T/G mismatch-specific endonuclease activity; IDA:EcoliWiki.
DR GO; GO:0006304; P:DNA modification; IEA:InterPro.
DR GO; GO:0006298; P:mismatch repair; IDA:EcoliWiki.
DR GO; GO:0000018; P:regulation of DNA recombination; IMP:EcoliWiki.
DR CDD; cd00583; MutH_Sau3AI; 1.
DR Gene3D; 3.40.600.10; -; 1.
DR HAMAP; MF_00759; MutH; 1.
DR InterPro; IPR004230; DNA_mismatch_repair_MutH.
DR InterPro; IPR011337; DNA_rep_MutH/RE_typeII_Sau3AI.
DR InterPro; IPR037057; DNA_rep_MutH/T2_RE_sf.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR Pfam; PF02976; MutH; 1.
DR SMART; SM00927; MutH; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
DR TIGRFAMs; TIGR02248; mutH_TIGR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Direct protein sequencing; DNA damage; DNA repair;
KW Endonuclease; Hydrolase; Nuclease; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:3031619"
FT CHAIN 2..229
FT /note="DNA mismatch repair protein MutH"
FT /id="PRO_0000198665"
FT VARIANT 58
FT /note="W -> S (in strain: ECOR 67)"
FT VARIANT 60
FT /note="G -> C (in strain: ECOR 13, ECOR 20, ECOR 26 and
FT ECOR 70)"
FT VARIANT 64
FT /note="G -> R (in strain: ECOR 50)"
FT VARIANT 135
FT /note="Q -> K (in strain: ECOR 51, ECOR 52, ECOR 57, ECOR
FT 59, ECOR 62 and ECOR 64)"
FT VARIANT 153
FT /note="Q -> R (in strain: ECOR 67)"
FT VARIANT 155
FT /note="R -> C (in strain: ECOR 35)"
FT MUTAGEN 28
FT /note="T->L: In TS7; temperature-sensitive."
FT MUTAGEN 157
FT /note="D->N: In TS28; temperature-sensitive."
FT HELIX 14..23
FT /evidence="ECO:0007829|PDB:1AZO"
FT TURN 24..26
FT /evidence="ECO:0007829|PDB:1AZO"
FT HELIX 29..35
FT /evidence="ECO:0007829|PDB:1AZO"
FT TURN 44..46
FT /evidence="ECO:0007829|PDB:1AZO"
FT HELIX 50..58
FT /evidence="ECO:0007829|PDB:1AZO"
FT HELIX 69..72
FT /evidence="ECO:0007829|PDB:1AZO"
FT STRAND 77..83
FT /evidence="ECO:0007829|PDB:1AZO"
FT STRAND 93..97
FT /evidence="ECO:0007829|PDB:1AZO"
FT STRAND 100..102
FT /evidence="ECO:0007829|PDB:1AZO"
FT TURN 108..110
FT /evidence="ECO:0007829|PDB:1AZO"
FT HELIX 112..116
FT /evidence="ECO:0007829|PDB:1AZO"
FT STRAND 118..126
FT /evidence="ECO:0007829|PDB:1AZO"
FT HELIX 133..135
FT /evidence="ECO:0007829|PDB:1AZO"
FT STRAND 142..144
FT /evidence="ECO:0007829|PDB:1AZO"
FT HELIX 148..166
FT /evidence="ECO:0007829|PDB:1AZO"
FT HELIX 170..172
FT /evidence="ECO:0007829|PDB:1AZO"
FT STRAND 179..184
FT /evidence="ECO:0007829|PDB:1AZO"
FT STRAND 194..197
FT /evidence="ECO:0007829|PDB:1AZO"
FT STRAND 203..206
FT /evidence="ECO:0007829|PDB:1AZO"
FT STRAND 210..213
FT /evidence="ECO:0007829|PDB:1AZO"
FT HELIX 215..224
FT /evidence="ECO:0007829|PDB:1AZO"
SQ SEQUENCE 229 AA; 25527 MW; 32543989D710A8FD CRC64;
MSQPRPLLSP PETEEQLLAQ AQQLSGYTLG ELAALVGLVT PENLKRDKGW IGVLLEIWLG
ASAGSKPEQD FAALGVELKT IPVDSLGRPL ETTFVCVAPL TGNSGVTWET SHVRHKLKRV
LWIPVEGERS IPLAQRRVGS PLLWSPNEEE DRQLREDWEE LMDMIVLGQV ERITARHGEY
LQIRPKAANA KALTEAIGAR GERILTLPRG FYLKKNFTSA LLARHFLIQ
