ID   MUTS2_ACET2             Reviewed;         793 AA.
AC   A3DE67;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Endonuclease MutS2 {ECO:0000255|HAMAP-Rule:MF_00092};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00092};
GN   Name=mutS2 {ECO:0000255|HAMAP-Rule:MF_00092}; OrderedLocusNames=Cthe_1014;
OS   Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS   103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC   Acetivibrio.
OX   NCBI_TaxID=203119;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC   B-4536 / VPI 7372;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA   Newcomb M., Richardson P.;
RT   "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL   Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Endonuclease that is involved in the suppression of
CC       homologous recombination and may therefore have a key role in the
CC       control of bacterial genetic diversity. {ECO:0000255|HAMAP-
CC       Rule:MF_00092}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00092}.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MutS2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00092}.
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DR   EMBL; CP000568; ABN52246.1; -; Genomic_DNA.
DR   RefSeq; WP_004463478.1; NC_009012.1.
DR   AlphaFoldDB; A3DE67; -.
DR   SMR; A3DE67; -.
DR   STRING; 203119.Cthe_1014; -.
DR   EnsemblBacteria; ABN52246; ABN52246; Cthe_1014.
DR   KEGG; cth:Cthe_1014; -.
DR   eggNOG; COG1193; Bacteria.
DR   HOGENOM; CLU_011252_2_1_9; -.
DR   OMA; IHAIIND; -.
DR   OrthoDB; 256392at2; -.
DR   Proteomes; UP000002145; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   GO; GO:0045910; P:negative regulation of DNA recombination; IEA:InterPro.
DR   CDD; cd03280; ABC_MutS2; 1.
DR   Gene3D; 3.30.1370.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00092; MutS2; 1.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR   InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR   InterPro; IPR005747; MutS2.
DR   InterPro; IPR045076; MutS_family.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002625; Smr_dom.
DR   InterPro; IPR036063; Smr_dom_sf.
DR   PANTHER; PTHR11361; PTHR11361; 1.
DR   PANTHER; PTHR11361:SF14; PTHR11361:SF14; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   Pfam; PF01713; Smr; 1.
DR   PIRSF; PIRSF005814; MutS_YshD; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SMART; SM00533; MUTSd; 1.
DR   SMART; SM00463; SMR; 1.
DR   SUPFAM; SSF160443; SSF160443; 1.
DR   SUPFAM; SSF48334; SSF48334; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01069; mutS2; 1.
DR   PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
DR   PROSITE; PS50828; SMR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..793
FT                   /note="Endonuclease MutS2"
FT                   /id="PRO_1000093356"
FT   DOMAIN          718..793
FT                   /note="Smr"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
FT   BINDING         335..342
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
SQ   SEQUENCE   793 AA;  88690 MW;  5B0DCC0B54BF7D63 CRC64;
     MNEKTLKILE FNKIIDKLVS LATSSLGKEL AEKLVPDTDL NRVERAQKET SDAVAFIARR
     GTPPMGGIHD IRDSLKRVEI GAILNPGELL KTADVLRAVR NLKSYASNDR IKTDEDNIVS
     ELIGCLESNK RIEDRIYMSI LSEDEIADNA SPTLANIRRQ IRNAQESIKD KLNDIIRSSR
     YQKYIQEPIV TLRGDRYVIP VKQEYRTEIP GLIHDSSASG ATIFIEPMAV VEANNHIREL
     KIKEQAEIEK ILGELTGEIR GIVDSLKSNV SILGRLDFIF AKARLSLDYN CVCPVLNDEH
     KILIKKGRHP LLDKKTVVPI DFWIGEDFNT LVVTGPNTGG KTVTLKTVGL FTLMTQAGLH
     IPANEGTKMS IFKKVYADIG DEQSIEQSLS TFSSHMKNIV GILKDVDEDS LVLFDELGAG
     TDPTEGAALA MSILEYLRNK GSTTVATTHY SQLKAYAVTT KFVENACCEF NVETLRPTYR
     LLIGVPGKSN AFAISKRLGL FDDIIEKAKE FLTQDDIKFE DMLMSIEKNL NQSENEKMKA
     ESYRLEAEKL KKELEEQKRK LAENRERLIQ EARAEARKIL LEARKEAEEI ISKMRRLEQE
     VHNAQRQKEA EELRLKLKRK VDSIEETLEL PLAPKNALVK PPENLKPGDS VLIVNLDQKG
     TVITPPDKDG EVVVQAGIMK INVHISNLKL VDEQKIVLNN SGIGKIGMSK AKSISTEIDV
     RGYNLEEAIE SVDKYLDDAY LSGLTEVSII HGKGTGVLRS GIQKFLKSDS RVKSFRLGKY
     GEGESGVTIV ELR