ID   MUTS2_CALBD             Reviewed;         787 AA.
AC   B9MK78;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Endonuclease MutS2 {ECO:0000255|HAMAP-Rule:MF_00092};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00092};
GN   Name=mutS2 {ECO:0000255|HAMAP-Rule:MF_00092}; OrderedLocusNames=Athe_1642;
OS   Caldicellulosiruptor bescii (strain ATCC BAA-1888 / DSM 6725 / Z-1320)
OS   (Anaerocellum thermophilum).
OC   Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales;
OC   Thermoanaerobacterales Family III. Incertae Sedis; Caldicellulosiruptor.
OX   NCBI_TaxID=521460;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1888 / DSM 6725 / Z-1320;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D.,
RA   Goodwin L., Pitluck S., Sims D., Meincke L., Brettin T., Detter J.C.,
RA   Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.,
RA   Kataeva I., Adams M.W.W.;
RT   "Complete sequence of chromosome of Anaerocellum thermophilum DSM 6725.";
RL   Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Endonuclease that is involved in the suppression of
CC       homologous recombination and may therefore have a key role in the
CC       control of bacterial genetic diversity. {ECO:0000255|HAMAP-
CC       Rule:MF_00092}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00092}.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MutS2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00092}.
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DR   EMBL; CP001393; ACM60736.1; -; Genomic_DNA.
DR   RefSeq; WP_015908069.1; NC_012034.1.
DR   AlphaFoldDB; B9MK78; -.
DR   SMR; B9MK78; -.
DR   STRING; 521460.Athe_1642; -.
DR   EnsemblBacteria; ACM60736; ACM60736; Athe_1642.
DR   GeneID; 31772991; -.
DR   KEGG; ate:Athe_1642; -.
DR   eggNOG; COG1193; Bacteria.
DR   HOGENOM; CLU_011252_2_1_9; -.
DR   OMA; IHAIIND; -.
DR   OrthoDB; 256392at2; -.
DR   Proteomes; UP000007723; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   GO; GO:0045910; P:negative regulation of DNA recombination; IEA:InterPro.
DR   CDD; cd03280; ABC_MutS2; 1.
DR   Gene3D; 3.30.1370.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00092; MutS2; 1.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR   InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR   InterPro; IPR005747; MutS2.
DR   InterPro; IPR045076; MutS_family.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002625; Smr_dom.
DR   InterPro; IPR036063; Smr_dom_sf.
DR   PANTHER; PTHR11361; PTHR11361; 1.
DR   PANTHER; PTHR11361:SF14; PTHR11361:SF14; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   Pfam; PF01713; Smr; 1.
DR   PIRSF; PIRSF005814; MutS_YshD; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SMART; SM00533; MUTSd; 1.
DR   SMART; SM00463; SMR; 1.
DR   SUPFAM; SSF160443; SSF160443; 1.
DR   SUPFAM; SSF48334; SSF48334; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01069; mutS2; 1.
DR   PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
DR   PROSITE; PS50828; SMR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Nucleotide-binding.
FT   CHAIN           1..787
FT                   /note="Endonuclease MutS2"
FT                   /id="PRO_1000192209"
FT   DOMAIN          711..786
FT                   /note="Smr"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
FT   BINDING         331..338
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
SQ   SEQUENCE   787 AA;  89398 MW;  8D926370A973C928 CRC64;
     MNQKTLKALE YDKIVEILKN MAKSTPAKEY FENLIPSTNV ADIENELNKV DESYRYVLKY
     GNLPTLEFEN ILPSLKKSKL GATLNPHEIL QIGKVLKLSY EMRTYLSFTQ DFSFLESMKK
     RLVNLKEVIS RIDQTFLTPD EILDTASSKL KEIRDKIRKL ENKIRDELNS MIRDPKIQRF
     LQEPIITIRG EKLLLPVKAE FRNEVKGIVH DQSATGATLF VEPFVCVEIS NQIKILKNQE
     KEEIERILQE ISSLIASYCE VIETSFYALV ELDIVFTKAI WAKEMNASKP IINASGIINL
     KKARHPLIQK DKVVPIDIHL GKDFDVLIIT GPNTGGKTVT LKTVGLFCLL CQSGIFIPAD
     EGSELCIFQK IFADIGDDQS IVQSLSTFSA HMKNIIEITK NADDKTLVLL DEIGAGTDPE
     EGAALAKAIL KYLSEKGSKV IATTHYGELK IFAQQEDRFE NASCEFDVKT LKPTYRLLIG
     IPGRSNALVI SSNLGLDKGI VEMARGYLSQ KTIDLDRIIN EMEQKRKEAE ENLELAQKLK
     HEAQALKAAY EEEKKRFETE RERIRKKAIN EAKEIVESSQ YEIENLFKDL RKLAENLKEK
     EVLKELEEKK REYERLIQSI SQQVKQEAES KTKKTIQNLR LGQKVYVRSF DAEGFVESLP
     DSKGNLTVQI GIMKINVNLS DIEEVEGQDS KIYQIASRNV IIKEKNIDMS IDVRGKTSDD
     AILEVDKYLD DAYTAGLKQV TIIHGKGTGV LRQAIRNFLR RHPHVKSFRD GTYGEGEQGV
     TVVELKD