ID   MUTS2_CLOAB             Reviewed;         788 AA.
AC   Q97GM6;
DT   28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Endonuclease MutS2 {ECO:0000255|HAMAP-Rule:MF_00092};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00092};
GN   Name=mutS2 {ECO:0000255|HAMAP-Rule:MF_00092}; OrderedLocusNames=CA_C2340;
OS   Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710
OS   / VKM B-1787).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=272562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
RX   PubMed=11466286; DOI=10.1128/jb.183.16.4823-4838.2001;
RA   Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R.,
RA   Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F.,
RA   Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V.,
RA   Smith D.R.;
RT   "Genome sequence and comparative analysis of the solvent-producing
RT   bacterium Clostridium acetobutylicum.";
RL   J. Bacteriol. 183:4823-4838(2001).
CC   -!- FUNCTION: Endonuclease that is involved in the suppression of
CC       homologous recombination and may therefore have a key role in the
CC       control of bacterial genetic diversity. {ECO:0000255|HAMAP-
CC       Rule:MF_00092}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00092}.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MutS2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00092}.
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DR   EMBL; AE001437; AAK80296.1; -; Genomic_DNA.
DR   PIR; E97188; E97188.
DR   RefSeq; NP_348956.1; NC_003030.1.
DR   RefSeq; WP_010965637.1; NC_003030.1.
DR   AlphaFoldDB; Q97GM6; -.
DR   SMR; Q97GM6; -.
DR   STRING; 272562.CA_C2340; -.
DR   EnsemblBacteria; AAK80296; AAK80296; CA_C2340.
DR   GeneID; 44998815; -.
DR   KEGG; cac:CA_C2340; -.
DR   PATRIC; fig|272562.8.peg.2536; -.
DR   eggNOG; COG1193; Bacteria.
DR   HOGENOM; CLU_011252_2_1_9; -.
DR   OMA; IHAIIND; -.
DR   OrthoDB; 256392at2; -.
DR   Proteomes; UP000000814; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   GO; GO:0045910; P:negative regulation of DNA recombination; IEA:InterPro.
DR   CDD; cd03280; ABC_MutS2; 1.
DR   Gene3D; 3.30.1370.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00092; MutS2; 1.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR   InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR   InterPro; IPR005747; MutS2.
DR   InterPro; IPR045076; MutS_family.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002625; Smr_dom.
DR   InterPro; IPR036063; Smr_dom_sf.
DR   PANTHER; PTHR11361; PTHR11361; 1.
DR   PANTHER; PTHR11361:SF14; PTHR11361:SF14; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   Pfam; PF01713; Smr; 1.
DR   PIRSF; PIRSF005814; MutS_YshD; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SMART; SM00533; MUTSd; 1.
DR   SMART; SM00463; SMR; 1.
DR   SUPFAM; SSF160443; SSF160443; 1.
DR   SUPFAM; SSF48334; SSF48334; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01069; mutS2; 1.
DR   PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
DR   PROSITE; PS50828; SMR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..788
FT                   /note="Endonuclease MutS2"
FT                   /id="PRO_0000115219"
FT   DOMAIN          713..788
FT                   /note="Smr"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
FT   BINDING         332..339
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
SQ   SEQUENCE   788 AA;  88271 MW;  3726BEFDAB395517 CRC64;
     MNEKSLRVLE YFKIKDGIKK YISTSAAKKL IDELEPYGSL YEVKEHIEET KEAFELLMKK
     GAPPFEGAYD VTEAVSMAEK GFSLAPGQLL KVGSMLRCAR KFKEYISHKE EEESYRIIED
     ICSGIIPLKN IEDNIFNAII GEEEISDKAS TALYSIRRSL KDKNASIKDK VNAMMRSYSK
     YLQENLYTIR GERYVIPVKA EYKAQVPGLV HDQSSTGATL FIEPMGLVNL NNEIKELMLK
     EKAEIERILR ELSALIYKSI VAVKNNEKIV TELDFIFAKA KYASSINATA PHVNDKGVID
     IVMGRHPLID PAKVVPLNIY MGREFTSLVI TGPNTGGKTV TLKTTGLLEV MAMSGLMIPA
     RENSTISFFK EVYADIGDEQ SIEQSLSTFS SHMTNIVRII DDADEDSLVL FDELGAGTDP
     TEGAALAISI LEALRKRGTK IVATTHYSEL KAYALKTENV ENASVEFDVE TLRPTYRLLI
     GIPGKSNAFE ISKRLGLSDY IIEEARKGIS KDTLEFEDLI QNLQTRSVKA EENLRKAEFL
     KEQAEKFKEK YEEKVSSITE TREKALHEGR REAKKIIEEA KSEADKILKD MREMERLGYS
     SEARQRLQES RQKLKEKLNN AEESLNISER DQGEALKSVK EGEEVFIPSL NMKGIVISTQ
     DSKGEVGIQA GIMKINVKLK DLRKTNNNPI SKKEKAVKKR EARLNLKSVA QSIDLRGLDS
     EEAIYKTDIY LDEAYMAGLG SVTVIHGKGT GVLRNAINTM LKKNSHVKSY RLGNFGEGGT
     GVTVVELK