ID   MUTS2_CLOBA             Reviewed;         785 AA.
AC   B2V583;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Endonuclease MutS2 {ECO:0000255|HAMAP-Rule:MF_00092};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00092};
GN   Name=mutS2 {ECO:0000255|HAMAP-Rule:MF_00092}; OrderedLocusNames=CLH_2231;
OS   Clostridium botulinum (strain Alaska E43 / Type E3).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=508767;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Alaska E43 / Type E3;
RA   Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A.,
RA   Smith T.J., Sutton G., Brettin T.S.;
RT   "Complete genome sequence of Clostridium botulinum E3 str. Alaska E43.";
RL   Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Endonuclease that is involved in the suppression of
CC       homologous recombination and may therefore have a key role in the
CC       control of bacterial genetic diversity. {ECO:0000255|HAMAP-
CC       Rule:MF_00092}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00092}.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MutS2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00092}.
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DR   EMBL; CP001078; ACD53455.1; -; Genomic_DNA.
DR   RefSeq; WP_012451352.1; NC_010723.1.
DR   AlphaFoldDB; B2V583; -.
DR   SMR; B2V583; -.
DR   KEGG; cbt:CLH_2231; -.
DR   HOGENOM; CLU_011252_2_1_9; -.
DR   OMA; IHAIIND; -.
DR   OrthoDB; 256392at2; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   GO; GO:0045910; P:negative regulation of DNA recombination; IEA:InterPro.
DR   CDD; cd03280; ABC_MutS2; 1.
DR   Gene3D; 3.30.1370.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00092; MutS2; 1.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR   InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR   InterPro; IPR005747; MutS2.
DR   InterPro; IPR045076; MutS_family.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002625; Smr_dom.
DR   InterPro; IPR036063; Smr_dom_sf.
DR   PANTHER; PTHR11361; PTHR11361; 1.
DR   PANTHER; PTHR11361:SF14; PTHR11361:SF14; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   Pfam; PF01713; Smr; 1.
DR   PIRSF; PIRSF005814; MutS_YshD; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SMART; SM00533; MUTSd; 1.
DR   SMART; SM00463; SMR; 1.
DR   SUPFAM; SSF160443; SSF160443; 1.
DR   SUPFAM; SSF48334; SSF48334; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01069; mutS2; 1.
DR   PROSITE; PS50828; SMR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Nucleotide-binding.
FT   CHAIN           1..785
FT                   /note="Endonuclease MutS2"
FT                   /id="PRO_1000093346"
FT   DOMAIN          710..785
FT                   /note="Smr"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
FT   BINDING         332..339
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
SQ   SEQUENCE   785 AA;  88310 MW;  F3662F76C76EF846 CRC64;
     MNKRSLRVLE FNKVKEMLKK YAYSSSAKKL VDELVPYDNT YEINNRLEES NEALEILMKK
     GNPPIEGLCD IGDILQRAKK GGTLTPEQLL KVLGMLTATR RMQEFFKRED QEVSFPKLED
     LAYILAPIND LEKEIERSIL SEDEVSDNAS TTLYNIRRSL KEKNSSVREK INSIVRSNSK
     YLQDSLYTIR GDRYVIPVKA EYKSSVPGLV HDQSSTGATL FIEPMGLVNL NNEIKELMLK
     EKAEIDRVLS ALSLKVKMNA EHCESNFKIL TNLDFIFSKG KYACELNAIK PMVRDDGIFN
     IMSGRHPLIE KDKVVPLDVV LGDEFDTLMI TGPNTGGKTV TLKTVGLLHI MALSGLLIPA
     SSNSSVSFFK EVFADIGDEQ SIEQSLSTFS SHLTNIVNIM EYDNRQSLIL FDELGGGTDP
     AEGAALAIAI IENLSSKGAK LIATTHYSEL KAYALNKERV ENASVEFDIN TLRPTYRLLI
     GVPGKSNAFE ISKRIGLGKE VIDCAKNYMS KENLEFEGLI RNLQEKSIIA KKDARDAKVI
     KDEADNLKKK YEQKLERLEK VKDKAYMDAR EEAKKIVANA KDEADEILKA MRELEKLGIG
     SGGRQRLEEE RKKLKDSLEE KEKNLYKMKE NDGEVLEKVA LGMEAFLPSL NQTVVVISMP
     DNRGEVQVEA GIMKISVKLK DLRKTKQSKV EKVKKKRELK LHFSKVENRI DLRGLDAEEA
     CYRVDKYLDD AYMGNLGEVT IVHGKGTGIL RKAINDMLKR HVHVKNYRLG GYGEGGDGAT
     IVELK