ID   MUTS2_CLOBK             Reviewed;         788 AA.
AC   B1IMK5;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Endonuclease MutS2 {ECO:0000255|HAMAP-Rule:MF_00092};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00092};
GN   Name=mutS2 {ECO:0000255|HAMAP-Rule:MF_00092}; OrderedLocusNames=CLD_1418;
OS   Clostridium botulinum (strain Okra / Type B1).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=498213;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Okra / Type B1;
RX   PubMed=18060065; DOI=10.1371/journal.pone.0001271;
RA   Smith T.J., Hill K.K., Foley B.T., Detter J.C., Munk A.C., Bruce D.C.,
RA   Doggett N.A., Smith L.A., Marks J.D., Xie G., Brettin T.S.;
RT   "Analysis of the neurotoxin complex genes in Clostridium botulinum A1-A4
RT   and B1 strains: BoNT/A3, /Ba4 and /B1 clusters are located within
RT   plasmids.";
RL   PLoS ONE 2:E1271-E1271(2007).
CC   -!- FUNCTION: Endonuclease that is involved in the suppression of
CC       homologous recombination and may therefore have a key role in the
CC       control of bacterial genetic diversity. {ECO:0000255|HAMAP-
CC       Rule:MF_00092}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00092}.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MutS2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00092}.
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DR   EMBL; CP000939; ACA44194.1; -; Genomic_DNA.
DR   RefSeq; WP_003403349.1; NC_010516.1.
DR   AlphaFoldDB; B1IMK5; -.
DR   SMR; B1IMK5; -.
DR   EnsemblBacteria; ACA44194; ACA44194; CLD_1418.
DR   KEGG; cbb:CLD_1418; -.
DR   HOGENOM; CLU_011252_2_1_9; -.
DR   OMA; IHAIIND; -.
DR   Proteomes; UP000008541; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   GO; GO:0045910; P:negative regulation of DNA recombination; IEA:InterPro.
DR   CDD; cd03280; ABC_MutS2; 1.
DR   Gene3D; 3.30.1370.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00092; MutS2; 1.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR   InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR   InterPro; IPR005747; MutS2.
DR   InterPro; IPR045076; MutS_family.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002625; Smr_dom.
DR   InterPro; IPR036063; Smr_dom_sf.
DR   PANTHER; PTHR11361; PTHR11361; 1.
DR   PANTHER; PTHR11361:SF14; PTHR11361:SF14; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   Pfam; PF01713; Smr; 1.
DR   PIRSF; PIRSF005814; MutS_YshD; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SMART; SM00533; MUTSd; 1.
DR   SMART; SM00463; SMR; 1.
DR   SUPFAM; SSF160443; SSF160443; 1.
DR   SUPFAM; SSF48334; SSF48334; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01069; mutS2; 1.
DR   PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
DR   PROSITE; PS50828; SMR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Nucleotide-binding.
FT   CHAIN           1..788
FT                   /note="Endonuclease MutS2"
FT                   /id="PRO_1000093348"
FT   DOMAIN          713..788
FT                   /note="Smr"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
FT   BINDING         332..339
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
SQ   SEQUENCE   788 AA;  88722 MW;  AFF740F4D5EDE21E CRC64;
     MKDKSIKVLE FNKIQEFLKN YTCTKAAKDI IEDLKPYDSV YEVREHLEET KEAFKLLITK
     GAPPFEGVYD IRSGISLAEK RSTLLPGQLL KIAAVLRCAR RFKEYINHKE EEESYRVLEN
     ICEGIFSLPK IEEEIFNAIE GEDEIADRAS STLYNIRRSL KEKNYSVRDK INSLVRSYSS
     YLQENIYTVR GDRYVLPVKA EHKGAVPGLV HDQSSTGATL FIEPMSLVNL NNEIKELMLK
     EKAEIERILT VLSAKINANI TGVKTDANIV WELDFIFAKA KFASEYNCTC PTINDEGIVD
     IIEGRHPLID RREVVPISVK LGEEFTSLMI TGPNTGGKTV TLKTVGLIHL MAMSGLMIPA
     RENSVISYFN NVFADIGDEQ SIEQSLSTFS SHMKNIVEIM DKADENSLVL FDELGAGTDP
     TEGAALAISI LENLRKRGTK IIATTHYSEL KAYALKKEGV ENASVEFDVE TLRPTYRLLI
     GIPGKSNAFE ISKRLGLPDY IIDFARENIS NENIRFEELI ENLQEKSIKA QEDARLAENL
     KLERDKEKKK YEEKLEGLQK VRDNALIDAR REAKNIIKEA KEEADKILKD IRQLERMGYS
     SDARRKLEEE RKKLKDKLDS IEEKEIKTVH KGEALKNVKE GDEVLLASIN QKVIVLSKPD
     NKGDVLVQAG IMKITANIKD LRAAKGSNSN SSSSKIKKSK KLNLNLRRVE SSVDLRGMDA
     EEAIYTVDKY LDEAYLGGLG EVTIVHGKGT GVLRKTIMDM LKGHSHVKKY RLGEYGEGGT
     GVTVVELK