ID   MUTS2_CLOBL             Reviewed;         788 AA.
AC   A7GHZ0;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Endonuclease MutS2 {ECO:0000255|HAMAP-Rule:MF_00092};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00092};
GN   Name=mutS2 {ECO:0000255|HAMAP-Rule:MF_00092}; OrderedLocusNames=CLI_3181;
OS   Clostridium botulinum (strain Langeland / NCTC 10281 / Type F).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=441772;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Langeland / NCTC 10281 / Type F;
RA   Brinkac L.M., Daugherty S., Dodson R.J., Madupu R., Brown J.L., Bruce D.,
RA   Detter C., Munk C., Smith L.A., Smith T.J., White O., Brettin T.S.;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Endonuclease that is involved in the suppression of
CC       homologous recombination and may therefore have a key role in the
CC       control of bacterial genetic diversity. {ECO:0000255|HAMAP-
CC       Rule:MF_00092}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00092}.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MutS2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00092}.
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DR   EMBL; CP000728; ABS42241.1; -; Genomic_DNA.
DR   RefSeq; WP_012100828.1; NC_009699.1.
DR   AlphaFoldDB; A7GHZ0; -.
DR   SMR; A7GHZ0; -.
DR   EnsemblBacteria; ABS42241; ABS42241; CLI_3181.
DR   KEGG; cbf:CLI_3181; -.
DR   HOGENOM; CLU_011252_2_1_9; -.
DR   OMA; IHAIIND; -.
DR   Proteomes; UP000002410; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   GO; GO:0045910; P:negative regulation of DNA recombination; IEA:InterPro.
DR   CDD; cd03280; ABC_MutS2; 1.
DR   Gene3D; 3.30.1370.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00092; MutS2; 1.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR   InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR   InterPro; IPR005747; MutS2.
DR   InterPro; IPR045076; MutS_family.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002625; Smr_dom.
DR   InterPro; IPR036063; Smr_dom_sf.
DR   PANTHER; PTHR11361; PTHR11361; 1.
DR   PANTHER; PTHR11361:SF14; PTHR11361:SF14; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   Pfam; PF01713; Smr; 1.
DR   PIRSF; PIRSF005814; MutS_YshD; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SMART; SM00533; MUTSd; 1.
DR   SMART; SM00463; SMR; 1.
DR   SUPFAM; SSF160443; SSF160443; 1.
DR   SUPFAM; SSF48334; SSF48334; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01069; mutS2; 1.
DR   PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
DR   PROSITE; PS50828; SMR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Nucleotide-binding.
FT   CHAIN           1..788
FT                   /note="Endonuclease MutS2"
FT                   /id="PRO_1000075474"
FT   DOMAIN          713..788
FT                   /note="Smr"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
FT   BINDING         332..339
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
SQ   SEQUENCE   788 AA;  88721 MW;  A2BC4FB228AE6559 CRC64;
     MKDKSIKVLE FNKIQEILKN YTCTKAAKDI IEDLKPYDSV YEVREHLEEA KEAFKLLITK
     GAPPFEGVYD IRNGIYLAEK GSALLPGQLL KIAAVLRCAR RFKEYINHKE EEESYRVLEN
     ICEGIFSLPK IEEEIFNAIE GEDEIADRAS STLYNIRRSL KEKNYSVRDK INSLVRSYSS
     YLQENIYTVR GDRYVLPVKA EHKGAVPGLV HDQSSTGATL FIEPMSLVNL NNEIKELMLK
     EKAEIERILT VLSAKINANI TGVKTDANIV WELDFIFAKA KFASEYNCTC PTINNEGIVD
     IIEGRHPLID RREVVPISVK LGEEFTSLMI TGPNTGGKTV TLKTVGLIHL MAMSGLMIPA
     RENSVISYFN NVFADIGDEQ SIEQSLSTFS SHMKNIVEIM DKADENSLVL FDELGAGTDP
     TEGAALAISI LENLRKRGTK IIATTHYSEL KAYALRKEGV ENASVEFDVE TLRPTYRLLI
     GIPGKSNAFE ISKRLGLPDY IIDFARENIS NENIRFEELI ENLQEKSIKA QEDARLAENL
     KLERDKEKKK YEEKLEGLQK VRDNALIDAR REAKNIIKEA KEEADKILKD IRQLERMGYS
     SDARRKLEEE RKKLKDKLDS IEEKEIKTVH KGEALKNVKE GDEVLLASIN QKVIVLSKPD
     NKGDVLVQAG IMKITANIKD LRAAKGSNFN SSSSKIKKSK KLNLNLRRVE SSVDLRGMDA
     EEAIYTVDKY LDEAYLGGLG EVTIVHGKGT GVLRKTIMDM LKGHSHVKRH RLGEYGEGGT
     GVTVVELK