ID   MUTS2_CLOK5             Reviewed;         786 AA.
AC   A5N245;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Endonuclease MutS2 {ECO:0000255|HAMAP-Rule:MF_00092};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00092};
GN   Name=mutS2 {ECO:0000255|HAMAP-Rule:MF_00092}; OrderedLocusNames=CKL_3183;
OS   Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=431943;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8527 / DSM 555 / NCIMB 10680;
RX   PubMed=18218779; DOI=10.1073/pnas.0711093105;
RA   Seedorf H., Fricke W.F., Veith B., Brueggemann H., Liesegang H.,
RA   Strittmatter A., Miethke M., Buckel W., Hinderberger J., Li F.,
RA   Hagemeier C., Thauer R.K., Gottschalk G.;
RT   "The genome of Clostridium kluyveri, a strict anaerobe with unique
RT   metabolic features.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:2128-2133(2008).
CC   -!- FUNCTION: Endonuclease that is involved in the suppression of
CC       homologous recombination and may therefore have a key role in the
CC       control of bacterial genetic diversity. {ECO:0000255|HAMAP-
CC       Rule:MF_00092}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00092}.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MutS2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00092}.
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DR   EMBL; CP000673; EDK35191.1; -; Genomic_DNA.
DR   RefSeq; WP_012103528.1; NC_009706.1.
DR   AlphaFoldDB; A5N245; -.
DR   SMR; A5N245; -.
DR   STRING; 431943.CKL_3183; -.
DR   EnsemblBacteria; EDK35191; EDK35191; CKL_3183.
DR   KEGG; ckl:CKL_3183; -.
DR   eggNOG; COG1193; Bacteria.
DR   HOGENOM; CLU_011252_2_1_9; -.
DR   OMA; IHAIIND; -.
DR   OrthoDB; 256392at2; -.
DR   Proteomes; UP000002411; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   GO; GO:0045910; P:negative regulation of DNA recombination; IEA:InterPro.
DR   CDD; cd03280; ABC_MutS2; 1.
DR   Gene3D; 3.30.1370.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00092; MutS2; 1.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR   InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR   InterPro; IPR005747; MutS2.
DR   InterPro; IPR045076; MutS_family.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002625; Smr_dom.
DR   InterPro; IPR036063; Smr_dom_sf.
DR   PANTHER; PTHR11361; PTHR11361; 1.
DR   PANTHER; PTHR11361:SF14; PTHR11361:SF14; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   Pfam; PF01713; Smr; 1.
DR   PIRSF; PIRSF005814; MutS_YshD; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SMART; SM00533; MUTSd; 1.
DR   SMART; SM00463; SMR; 1.
DR   SUPFAM; SSF160443; SSF160443; 1.
DR   SUPFAM; SSF48334; SSF48334; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01069; mutS2; 1.
DR   PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
DR   PROSITE; PS50828; SMR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..786
FT                   /note="Endonuclease MutS2"
FT                   /id="PRO_1000093351"
FT   DOMAIN          711..786
FT                   /note="Smr"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
FT   BINDING         332..339
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
SQ   SEQUENCE   786 AA;  88552 MW;  6C716BCE6ECEBAAB CRC64;
     MNEKSLRILE FYKVKDELKK YTNTNAAKDL VDNLKPYDNI HDVREHLEET EEALKLIISR
     GNPPFDGVYD VRQGVKMAQK GSILMPAQIF RIGAILKASR RFEKYIKAKG EGEGFRIIED
     ICQGIVLLKG LEDKIFISIE SEDEISDRAS SLLYNIRKSI RDKNASVRDK VNSLIRNYSS
     YLQDNLYTIR GDRYVLPVRA ENKALVPGLV HDQSSSGSTL YIEPMALVNL NNEIKELKLK
     EKAEIDRILY ELSKEIHDNI VVIKNNADII WELDFIFAKA KFGSELNGNI PIVNDNCIID
     IVEGRHPLID RKTVVPMDVY MGKDFTCLVI TGPNTGGKTV ALKTMGLLHI MALSGLMIPA
     RENSTVGFFT EIFADIGDEQ SIEQNLSTFS SHMTNIINII NNSDEKSLIL FDELGAGTDP
     TEGAALAVSI LENLKDRGSM IVATTHYSEL KAYALKSNGV ENASVEFDVD TLKPTYKLMI
     GIPGKSNAFE ISKRLGLPEF IIKAARENIA SEALKFEDLI QSLQEKRIKA ENYFREAEIL
     KREAAKIKEK YEQKAIRLQE VRDKSITEAH RKAREIIRES KEEADRILKD IRELEKMGYS
     SSVKHELEER RKMLKDKLEN VEENLYKAKS EDGQRLKSVK EGEEVFIPSL NQKVLVLSKP
     DNKGEVQVQA GIMKISVNLK ELRAPKGSTK NTDKKLKREA NLNLRSVATS VDLRGMDSIE
     AAYITDKYLD DAYVAGLKEV TIIHGKGTGI LRSSITNMLK SHSHVKNYRI GEYGEGGTGV
     TIVELK