ID   MUTS2_CLOTE             Reviewed;         786 AA.
AC   Q891U1;
DT   28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Endonuclease MutS2 {ECO:0000255|HAMAP-Rule:MF_00092};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00092};
GN   Name=mutS2 {ECO:0000255|HAMAP-Rule:MF_00092}; Synonyms=mutS;
GN   OrderedLocusNames=CTC_02274;
OS   Clostridium tetani (strain Massachusetts / E88).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=212717;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Massachusetts / E88;
RX   PubMed=12552129; DOI=10.1073/pnas.0335853100;
RA   Brueggemann H., Baeumer S., Fricke W.F., Wiezer A., Liesegang H.,
RA   Decker I., Herzberg C., Martinez-Arias R., Merkl R., Henne A.,
RA   Gottschalk G.;
RT   "The genome sequence of Clostridium tetani, the causative agent of tetanus
RT   disease.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:1316-1321(2003).
CC   -!- FUNCTION: Endonuclease that is involved in the suppression of
CC       homologous recombination and may therefore have a key role in the
CC       control of bacterial genetic diversity. {ECO:0000255|HAMAP-
CC       Rule:MF_00092}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00092}.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MutS2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00092}.
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DR   EMBL; AE015927; AAO36754.1; -; Genomic_DNA.
DR   RefSeq; WP_011100415.1; NC_004557.1.
DR   AlphaFoldDB; Q891U1; -.
DR   SMR; Q891U1; -.
DR   STRING; 212717.CTC_02274; -.
DR   EnsemblBacteria; AAO36754; AAO36754; CTC_02274.
DR   GeneID; 64179651; -.
DR   KEGG; ctc:CTC_02274; -.
DR   HOGENOM; CLU_011252_2_1_9; -.
DR   OMA; IHAIIND; -.
DR   OrthoDB; 256392at2; -.
DR   Proteomes; UP000001412; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   GO; GO:0045910; P:negative regulation of DNA recombination; IEA:InterPro.
DR   CDD; cd03280; ABC_MutS2; 1.
DR   Gene3D; 3.30.1370.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00092; MutS2; 1.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR   InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR   InterPro; IPR005747; MutS2.
DR   InterPro; IPR045076; MutS_family.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002625; Smr_dom.
DR   InterPro; IPR036063; Smr_dom_sf.
DR   PANTHER; PTHR11361; PTHR11361; 1.
DR   PANTHER; PTHR11361:SF14; PTHR11361:SF14; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   Pfam; PF01713; Smr; 1.
DR   PIRSF; PIRSF005814; MutS_YshD; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SMART; SM00533; MUTSd; 1.
DR   SMART; SM00463; SMR; 1.
DR   SUPFAM; SSF160443; SSF160443; 1.
DR   SUPFAM; SSF48334; SSF48334; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01069; mutS2; 1.
DR   PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
DR   PROSITE; PS50828; SMR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..786
FT                   /note="Endonuclease MutS2"
FT                   /id="PRO_0000115221"
FT   DOMAIN          711..786
FT                   /note="Smr"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
FT   BINDING         332..339
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
SQ   SEQUENCE   786 AA;  89224 MW;  7FB763E5C17F2683 CRC64;
     MNERALKVLE FYKIKEKLKG FVKTNAAKDI VDNLKPYTNL YEVEMHLEET MEALDLLQTK
     GEPPFEGVYD IRDILIRVEK AMVLLPGQLL KVASILKSSR RFKDYVGRKH EETPHKNIED
     ICEGIELLKG LEESIFNIVI SDDEIADRAS LKLYGIRRSL RDKNDSLRDR VNSLVRKYSP
     YLQESIYTIR GDRYVLPVRA EHKGSVPGLV HDQSSSGATL FIEPMSLVNL NNEIKELLLK
     EREEIERILE ELSLKVYNNL DVVNVNAKVI WELDFIFAKA KFASHNNCTL PKVSEDGIVD
     IIEGKHPLID TEKVVPTDIY LGKDFTSLII TGPNTGGKTV TLKTLGLIHI MGLSGLLIPA
     KENSTIAFFE EIFADIGDEQ SIEQSLSTFS SHMVNIVDII SKADHKSLVL FDELGAGTDP
     TEGAALAISI LENLRRRKCK IVATTHYSEL KGYALKTEDV ENASVEFDVE TLRPTYRLLI
     GIPGKSNAFE ISKRLGLGED IIEEAKNNIE SDSLKFEALI EKLQSKSIKA SDDARRAEMY
     KLEADRLKDK YEDKLKIVEE TREKLLKGAQ EEAKKLIKEA KEEADEILKN IRELEKMGYS
     STARQKLEEE RKKLNSKIHK LEEKEENLNK EKGKKIKEIN LGEEVYLPKL DQKVIILSKP
     DNRGEVQVQA GIMKINVKLD DLRLVDKPKE ENKKGRKREA KLRMRTVESE IDLRGMDSME
     AVYNTDKYLD EAYVAGLKEV TVIHGKGTGI LRKSINDMLK RHPQVKNYRL GEYGEGGTGV
     TVVELK