ID   MUTS2_ENTFA             Reviewed;         788 AA.
AC   Q835H3;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Endonuclease MutS2 {ECO:0000255|HAMAP-Rule:MF_00092};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00092};
GN   Name=mutS2 {ECO:0000255|HAMAP-Rule:MF_00092}; OrderedLocusNames=EF_1404;
OS   Enterococcus faecalis (strain ATCC 700802 / V583).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=226185;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700802 / V583;
RX   PubMed=12663927; DOI=10.1126/science.1080613;
RA   Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R.,
RA   Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H.,
RA   Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C.,
RA   DeBoy R.T., Durkin S.A., Kolonay J.F., Madupu R., Nelson W.C.,
RA   Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M.,
RA   Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.;
RT   "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus
RT   faecalis.";
RL   Science 299:2071-2074(2003).
CC   -!- FUNCTION: Endonuclease that is involved in the suppression of
CC       homologous recombination and may therefore have a key role in the
CC       control of bacterial genetic diversity. {ECO:0000255|HAMAP-
CC       Rule:MF_00092}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00092}.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MutS2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00092}.
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DR   EMBL; AE016830; AAO81195.1; -; Genomic_DNA.
DR   RefSeq; NP_815125.1; NC_004668.1.
DR   RefSeq; WP_002382291.1; NZ_KE136528.1.
DR   AlphaFoldDB; Q835H3; -.
DR   SMR; Q835H3; -.
DR   STRING; 226185.EF_1404; -.
DR   DNASU; 1200304; -.
DR   EnsemblBacteria; AAO81195; AAO81195; EF_1404.
DR   KEGG; efa:EF1404; -.
DR   PATRIC; fig|226185.45.peg.2097; -.
DR   eggNOG; COG1193; Bacteria.
DR   HOGENOM; CLU_011252_2_1_9; -.
DR   OMA; IHAIIND; -.
DR   Proteomes; UP000001415; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   GO; GO:0045910; P:negative regulation of DNA recombination; IEA:InterPro.
DR   CDD; cd03280; ABC_MutS2; 1.
DR   Gene3D; 3.30.1370.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00092; MutS2; 1.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR   InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR   InterPro; IPR005747; MutS2.
DR   InterPro; IPR045076; MutS_family.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002625; Smr_dom.
DR   InterPro; IPR036063; Smr_dom_sf.
DR   PANTHER; PTHR11361; PTHR11361; 1.
DR   PANTHER; PTHR11361:SF14; PTHR11361:SF14; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   Pfam; PF01713; Smr; 1.
DR   PIRSF; PIRSF005814; MutS_YshD; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SMART; SM00533; MUTSd; 1.
DR   SMART; SM00463; SMR; 1.
DR   SUPFAM; SSF160443; SSF160443; 1.
DR   SUPFAM; SSF48334; SSF48334; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01069; mutS2; 1.
DR   PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
DR   PROSITE; PS50828; SMR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..788
FT                   /note="Endonuclease MutS2"
FT                   /id="PRO_1000075475"
FT   DOMAIN          713..788
FT                   /note="Smr"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
FT   BINDING         334..341
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
SQ   SEQUENCE   788 AA;  88311 MW;  5555AB7704C1FE32 CRC64;
     MNQRILSTLG FDKVKQQLLQ FIVTAQGTNE VSELLPIADE NKIQSWLNET QDGLKVQRLR
     GGIPIPKLEN IQPHMKRIEI GADLNGIELA QVGRVLSTTS ELTRFFDELS ENEVDFERLY
     MWREQLEVLP ELNRQLKQAI DDDGYVTDEA SPALKAIRQN IRRSEQTIRE ELDSIIRGKN
     ARYLSDALVT MRNERYVIPV KQEYKNIFGG VVHDQSASGQ TLFIEPKQIL EMNNRLRQQQ
     IAERNEITRI LAELSAELVP YRREITHNAY VIGKLDFINA KARLGKELKA VVPEISQANH
     VVFKQARHPL LNPEKAVAND IVIGEEYQAI VITGPNTGGK TITLKTLGLL QLMGQAGLPI
     PVEEESKMGI FTEVFADIGD EQSIEQSLST FSSHMTNIVS VLKKVDHQSL VLFDELGAGT
     DPQEGAALAI AILDSLGAKG AYVMATTHYP ELKVYGYNRA GTINASMEFD VDTLSPTYRL
     LIGVPGRSNA FEISKRLGLD NSIIEAAKQI MDGESQDLNE MIEDLENRRK MAETEYLEAR
     HYVDESAALH KELKEAYQVF FEEREKELQK ARKEANKIIA EAEENAETII SDIRKMQLES
     GQQGGVKEHQ LIDAKTQLSQ LHHEETKLAK NKVLKKAKEQ KKLKAGDEVI VNTYGQRGTL
     LKDNGKGQWQ VQLGILKMNV SEEDMTPVAP QKEAKPRVTT VRSAESSHVG TQLDLRGKRY
     EEALAEVDQY IDAAILAGYP QVTIVHGKGT GALRTGITEF LKNHRSVKSY EFAPQNQGGN
     GATVVKFQ