ID   MUTS2_LACAC             Reviewed;         785 AA.
AC   Q5FLW2;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Endonuclease MutS2 {ECO:0000255|HAMAP-Rule:MF_00092};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00092};
GN   Name=mutS2 {ECO:0000255|HAMAP-Rule:MF_00092}; OrderedLocusNames=LBA0421;
OS   Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=272621;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700396 / NCK56 / N2 / NCFM;
RX   PubMed=15671160; DOI=10.1073/pnas.0409188102;
RA   Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L.,
RA   McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S.,
RA   Hamrick A., Cano R., Klaenhammer T.R.;
RT   "Complete genome sequence of the probiotic lactic acid bacterium
RT   Lactobacillus acidophilus NCFM.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005).
CC   -!- FUNCTION: Endonuclease that is involved in the suppression of
CC       homologous recombination and may therefore have a key role in the
CC       control of bacterial genetic diversity. {ECO:0000255|HAMAP-
CC       Rule:MF_00092}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00092}.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MutS2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00092}.
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DR   EMBL; CP000033; AAV42312.1; -; Genomic_DNA.
DR   RefSeq; WP_003549197.1; NC_006814.3.
DR   RefSeq; YP_193343.1; NC_006814.3.
DR   AlphaFoldDB; Q5FLW2; -.
DR   SMR; Q5FLW2; -.
DR   STRING; 272621.LBA0421; -.
DR   PRIDE; Q5FLW2; -.
DR   EnsemblBacteria; AAV42312; AAV42312; LBA0421.
DR   GeneID; 56942026; -.
DR   KEGG; lac:LBA0421; -.
DR   PATRIC; fig|272621.13.peg.406; -.
DR   eggNOG; COG1193; Bacteria.
DR   HOGENOM; CLU_011252_2_1_9; -.
DR   OMA; IHAIIND; -.
DR   BioCyc; LACI272621:G1G49-415-MON; -.
DR   Proteomes; UP000006381; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   GO; GO:0045910; P:negative regulation of DNA recombination; IEA:InterPro.
DR   CDD; cd03280; ABC_MutS2; 1.
DR   Gene3D; 3.30.1370.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00092; MutS2; 1.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR   InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR   InterPro; IPR005747; MutS2.
DR   InterPro; IPR045076; MutS_family.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002625; Smr_dom.
DR   InterPro; IPR036063; Smr_dom_sf.
DR   PANTHER; PTHR11361; PTHR11361; 1.
DR   PANTHER; PTHR11361:SF14; PTHR11361:SF14; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   Pfam; PF01713; Smr; 1.
DR   PIRSF; PIRSF005814; MutS_YshD; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SMART; SM00533; MUTSd; 1.
DR   SMART; SM00463; SMR; 1.
DR   SUPFAM; SSF160443; SSF160443; 1.
DR   SUPFAM; SSF48334; SSF48334; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01069; mutS2; 1.
DR   PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
DR   PROSITE; PS50828; SMR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..785
FT                   /note="Endonuclease MutS2"
FT                   /id="PRO_1000093360"
FT   DOMAIN          710..785
FT                   /note="Smr"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
FT   BINDING         333..340
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
SQ   SEQUENCE   785 AA;  88146 MW;  CA3A5E3645D16A1C CRC64;
     MNNKILKILE FGEITDRLGA LAITSPAKER AEKLLPSSDF DQVQNDIKQT LALTNLLRIK
     GQLPLTNFKD VRPSTKRLGV KANLNAQELG NLLLVLSLAQ EINEFLEDVD EKVDLTIIDP
     ILDQLDVPDL LFRELKKSID YDGEVLDTAS SELARLRHDI ASNEEDIKNR MTTYTKGNSS
     KYLSEQIVTI RDDRYVIPVK QEYRAKFGGV VHDQSASGQT LFVEPEAVLN LNNRQQNLIA
     KEKQEIRNIL KHLSNIARED IDSLNNIASA LTSLDFLQAK AKLAKEMKAS EPKLTKDHSL
     NLRNARHPLI NPEKVVPNNI RLGGDFDTML ITGPNTGGKT ITLKTAGLLQ LMAQSGLFIP
     AEEDSQVGVF EQIYADIGDE QSIEQSLSTF SSHMNDIIAI MKNVNSETLV LIDEIGAGTD
     PEEGASLAIS ILDFLRKKDA KIMVTTHYPE LKLYGYNRPR TTNASMEFDL KTLSPTYRLQ
     IGIPGHSNAF AIARRLGMRE DVVKNAQELM SDEDSDINKM IAKLNAQTKA ATTARNRLET
     SLDRSQKLEQ KLQQALDWYN QRVQKQLDFA QERANEIIAK RRKKADKIIE QLEQQKNVGI
     KENKIIEAKG ELNSLERQAN NLAHNKVLQR EKRRHHVSIG DRVKVLSYGQ TGTITKQLSE
     HEYEVQMGII KVKVSDRDVE RIDNSQSTAK PKRLVRATSA VRRSNAHSEL DLRGQRYDEA
     MTNLDRYIDS VLLAGLDTVT IIHGIGTGAI RKGVWQYLRS SNHVKNFNYA PANEGGNGAT
     IVQLK