ID   MUTS2_LACE2             Reviewed;         787 AA.
AC   C4Z417;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Endonuclease MutS2 {ECO:0000255|HAMAP-Rule:MF_00092};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00092};
GN   Name=mutS2 {ECO:0000255|HAMAP-Rule:MF_00092};
GN   OrderedLocusNames=EUBELI_01853;
OS   Lachnospira eligens (strain ATCC 27750 / DSM 3376 / VPI C15-48 / C15-B4)
OS   (Eubacterium eligens).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae;
OC   Lachnospira.
OX   NCBI_TaxID=515620;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27750 / DSM 3376 / VPI C15-48 / C15-B4;
RX   PubMed=19321416; DOI=10.1073/pnas.0901529106;
RA   Mahowald M.A., Rey F.E., Seedorf H., Turnbaugh P.J., Fulton R.S.,
RA   Wollam A., Shah N., Wang C., Magrini V., Wilson R.K., Cantarel B.L.,
RA   Coutinho P.M., Henrissat B., Crock L.W., Russell A., Verberkmoes N.C.,
RA   Hettich R.L., Gordon J.I.;
RT   "Characterizing a model human gut microbiota composed of members of its two
RT   dominant bacterial phyla.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:5859-5864(2009).
CC   -!- FUNCTION: Endonuclease that is involved in the suppression of
CC       homologous recombination and may therefore have a key role in the
CC       control of bacterial genetic diversity. {ECO:0000255|HAMAP-
CC       Rule:MF_00092}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00092}.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MutS2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00092}.
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DR   EMBL; CP001104; ACR72842.1; -; Genomic_DNA.
DR   RefSeq; WP_012740074.1; NC_012778.1.
DR   AlphaFoldDB; C4Z417; -.
DR   SMR; C4Z417; -.
DR   STRING; 515620.EUBELI_01853; -.
DR   PRIDE; C4Z417; -.
DR   EnsemblBacteria; ACR72842; ACR72842; EUBELI_01853.
DR   GeneID; 41356503; -.
DR   KEGG; eel:EUBELI_01853; -.
DR   eggNOG; COG1193; Bacteria.
DR   HOGENOM; CLU_011252_2_1_9; -.
DR   OMA; IHAIIND; -.
DR   OrthoDB; 256392at2; -.
DR   Proteomes; UP000001476; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   GO; GO:0045910; P:negative regulation of DNA recombination; IEA:InterPro.
DR   CDD; cd03280; ABC_MutS2; 1.
DR   Gene3D; 3.30.1370.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00092; MutS2; 1.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR   InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR   InterPro; IPR005747; MutS2.
DR   InterPro; IPR045076; MutS_family.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002625; Smr_dom.
DR   InterPro; IPR036063; Smr_dom_sf.
DR   PANTHER; PTHR11361; PTHR11361; 1.
DR   PANTHER; PTHR11361:SF14; PTHR11361:SF14; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   Pfam; PF01713; Smr; 1.
DR   PIRSF; PIRSF005814; MutS_YshD; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SMART; SM00533; MUTSd; 1.
DR   SMART; SM00463; SMR; 1.
DR   SUPFAM; SSF160443; SSF160443; 1.
DR   SUPFAM; SSF48334; SSF48334; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01069; mutS2; 1.
DR   PROSITE; PS50828; SMR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..787
FT                   /note="Endonuclease MutS2"
FT                   /id="PRO_1000202679"
FT   DOMAIN          712..787
FT                   /note="Smr"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
FT   BINDING         329..336
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
SQ   SEQUENCE   787 AA;  86628 MW;  D63D9E9A1CAEF713 CRC64;
     MNKKSLSTLE FYKITDQLVS YACCDGAKKI LRNLKPMTDI TDINLRLNET NDALSRIFQK
     GTVDFSQTKD IRASVARLKV GSSLNISELL NISAILSCAK HVKDYYEHRE DSISGMLENL
     ATVDALNSQI KKCIISEDEI SDDASSNLRS IRRSKSIAND RIHSELNKLL NSPTYRTYLQ
     DYVITTRQGR YCLPVKAEYK SAFPGMIHDQ SSTGSTLFIE PAAVVKLNND IRELELKEAA
     EIEVILADLS AKAGEHTEEL LCDYEILVEL DCIFAKAQLA RHMHASRPVM NTSGIINIKK
     GRHPLIESHT VVPIDIYLGT DFKLLIITGP NTGGKTVSLK TVGLLTLMAQ SGLFIPALDH
     SDIAVFKNIY ADIGDEQSIE QSLSTFSSHM TNTVKILKEA DENCLVLFDE IGAGTDPTEG
     AALAIAILND LKMRGVTTMA TTHYSEIKLY ALSTEGVENA SCEFDVESLR PTYRLLIGIP
     GKSNAFAISK KLGLPDYILS DASERLNAED VHFEDIVSDL EHARISLEKE QAEVESYKAE
     IASLKEKLQA KNERLDERTD NIIRKANEQA AAILKDAKDF ADETIKAMNK HGMTVAELEK
     HRTAVREKMN KNQAKLKVEP AKVKAHKAHD ISEFKTGMHV KVLTMNVSGT VSAIHPAKKQ
     VTVQVGALST KIDIKNLEIL SDYKEPKEAP SKAAGGSGKI KMSKSAGIST EINLLGCTVD
     EAVARLDKYL DDAYIARIPQ VRIVHGKGTG ALRNGVTAYL RGVPYIKSFR LGEIGEGDAG
     VTIVDFK