ID   MUTS2_LACH4             Reviewed;         785 AA.
AC   A8YTJ4;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Endonuclease MutS2 {ECO:0000255|HAMAP-Rule:MF_00092};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00092};
GN   Name=mutS2 {ECO:0000255|HAMAP-Rule:MF_00092}; OrderedLocusNames=lhv_0442;
OS   Lactobacillus helveticus (strain DPC 4571).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=405566;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DPC 4571;
RX   PubMed=17993529; DOI=10.1128/jb.01295-07;
RA   Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K.,
RA   McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F.,
RA   Beresford T., Ross R.P.;
RT   "Genome sequence of Lactobacillus helveticus: an organism distinguished by
RT   selective gene loss and IS element expansion.";
RL   J. Bacteriol. 190:727-735(2008).
CC   -!- FUNCTION: Endonuclease that is involved in the suppression of
CC       homologous recombination and may therefore have a key role in the
CC       control of bacterial genetic diversity. {ECO:0000255|HAMAP-
CC       Rule:MF_00092}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00092}.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MutS2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00092}.
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DR   EMBL; CP000517; ABX26650.1; -; Genomic_DNA.
DR   RefSeq; WP_012211453.1; NC_010080.1.
DR   AlphaFoldDB; A8YTJ4; -.
DR   SMR; A8YTJ4; -.
DR   STRING; 405566.lhv_0442; -.
DR   EnsemblBacteria; ABX26650; ABX26650; lhv_0442.
DR   KEGG; lhe:lhv_0442; -.
DR   eggNOG; COG1193; Bacteria.
DR   HOGENOM; CLU_011252_2_1_9; -.
DR   OMA; IHAIIND; -.
DR   Proteomes; UP000000790; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   GO; GO:0045910; P:negative regulation of DNA recombination; IEA:InterPro.
DR   CDD; cd03280; ABC_MutS2; 1.
DR   Gene3D; 3.30.1370.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00092; MutS2; 1.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR   InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR   InterPro; IPR005747; MutS2.
DR   InterPro; IPR045076; MutS_family.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002625; Smr_dom.
DR   InterPro; IPR036063; Smr_dom_sf.
DR   PANTHER; PTHR11361; PTHR11361; 1.
DR   PANTHER; PTHR11361:SF14; PTHR11361:SF14; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   Pfam; PF01713; Smr; 1.
DR   PIRSF; PIRSF005814; MutS_YshD; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SMART; SM00533; MUTSd; 1.
DR   SMART; SM00463; SMR; 1.
DR   SUPFAM; SSF160443; SSF160443; 1.
DR   SUPFAM; SSF48334; SSF48334; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01069; mutS2; 1.
DR   PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
DR   PROSITE; PS50828; SMR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Nucleotide-binding.
FT   CHAIN           1..785
FT                   /note="Endonuclease MutS2"
FT                   /id="PRO_1000093365"
FT   DOMAIN          710..785
FT                   /note="Smr"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
FT   BINDING         334..341
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
SQ   SEQUENCE   785 AA;  87959 MW;  897FF080988EBA72 CRC64;
     MNDKILKILE FGEITKRLSK LAITAPAKEA ALKLRPSSNF DQVQNELKQT LALADLLRVK
     GRLPLTDFQD VHPSTKRLGV KANLNAQELG NLLLVLSLAN EINKFLEDVD DEKLNLSAID
     SVLDQLDVPD LLFRELKKSV DYDGEVLDTA SNALARLRHD MRSNEEDIKN RMDAYTKGNS
     SKYLSEQIVT IRDDRYVIPV KQEYRGKFGG VVHDQSASGQ TLFIEPEAVL NLNNRQQNLI
     AQEKQEIRNI LKHLSSLARE EINSINNIAN SLTRLDFLQA KAKLAKEMKA SEPKLTQDHS
     LELRNARHPL IDPEKVVPND IRLGGDYDTM LITGPNTGGK TITLKTAGLL QLMAQSGLFI
     PAEEGSKVGV FKEVYADIGD EQSIEQSLST FSSHINDIIA IMKNVDKETL VLIDEIGAGT
     DPEEGASLAI SILDFLRKKD AKIMVTTHYP ELKLYGYNRP RTINASMEFD LKTLSPTYHL
     QIGIPGHSNA FAIARRLGMR EDVVKNAQNL MADEDSDINK MIAKLNAQTK AATTARNRLE
     TSLDRSQKLE QKLQQALDWY NQRVQKQLDF AQERANEVVA KRRKKADKII AELEKQKNVG
     VKENKIIEAK GELNSLERQA HNLAHNKVLQ REKRQHHVSV GDQVKVLSYG QTGTITKKLS
     EHEYEVQIGI IKVKVSDRDI ERIAKNNAQP KKKLVRATSA IRRSNAHSEL DLRGQRYDEA
     MTNLDRYIDS ALLAGLDIVT IIHGIGTGAI RKGVWQYLRS SNHVKGFNYA PANEGGNGAT
     IVKLK