ID   MUTS2_LACP7             Reviewed;         796 AA.
AC   A9KR74;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Endonuclease MutS2 {ECO:0000255|HAMAP-Rule:MF_00092};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00092};
GN   Name=mutS2 {ECO:0000255|HAMAP-Rule:MF_00092}; OrderedLocusNames=Cphy_0153;
OS   Lachnoclostridium phytofermentans (strain ATCC 700394 / DSM 18823 / ISDg)
OS   (Clostridium phytofermentans).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae.
OX   NCBI_TaxID=357809;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700394 / DSM 18823 / ISDg;
RA   Leschine S.B., Warnick T.A., Blanchard J.L., Schnell D.J., Petit E.L.,
RA   LaTouf W.G., Copeland A., Lucas S., Lapidus A., Barry K.,
RA   Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T.,
RA   Bruce D., Detter J.C., Han C., Kuske C., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Kim E.A., Richardson P.;
RT   "Complete genome sequence of Clostridium phytofermentans ISDg.";
RL   Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Endonuclease that is involved in the suppression of
CC       homologous recombination and may therefore have a key role in the
CC       control of bacterial genetic diversity. {ECO:0000255|HAMAP-
CC       Rule:MF_00092}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00092}.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MutS2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00092}.
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DR   EMBL; CP000885; ABX40542.1; -; Genomic_DNA.
DR   RefSeq; WP_012198185.1; NC_010001.1.
DR   AlphaFoldDB; A9KR74; -.
DR   SMR; A9KR74; -.
DR   STRING; 357809.Cphy_0153; -.
DR   PRIDE; A9KR74; -.
DR   EnsemblBacteria; ABX40542; ABX40542; Cphy_0153.
DR   KEGG; cpy:Cphy_0153; -.
DR   eggNOG; COG1193; Bacteria.
DR   HOGENOM; CLU_011252_2_1_9; -.
DR   OMA; IHAIIND; -.
DR   OrthoDB; 256392at2; -.
DR   Proteomes; UP000000370; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   GO; GO:0045910; P:negative regulation of DNA recombination; IEA:InterPro.
DR   CDD; cd03280; ABC_MutS2; 1.
DR   Gene3D; 3.30.1370.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00092; MutS2; 1.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR   InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR   InterPro; IPR005747; MutS2.
DR   InterPro; IPR045076; MutS_family.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002625; Smr_dom.
DR   InterPro; IPR036063; Smr_dom_sf.
DR   PANTHER; PTHR11361; PTHR11361; 1.
DR   PANTHER; PTHR11361:SF14; PTHR11361:SF14; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   Pfam; PF01713; Smr; 1.
DR   PIRSF; PIRSF005814; MutS_YshD; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SMART; SM00533; MUTSd; 1.
DR   SMART; SM00463; SMR; 1.
DR   SUPFAM; SSF160443; SSF160443; 1.
DR   SUPFAM; SSF48334; SSF48334; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01069; mutS2; 1.
DR   PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
DR   PROSITE; PS50828; SMR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..796
FT                   /note="Endonuclease MutS2"
FT                   /id="PRO_1000093354"
FT   DOMAIN          721..796
FT                   /note="Smr"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
FT   REGION          620..644
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         339..346
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
SQ   SEQUENCE   796 AA;  88400 MW;  5D8B27914D3B1857 CRC64;
     MNEKALRTLE YHKIIEKLSA LAGSSLGREK CHQLLPLVKL EDIVQMQQET TDALTRLYAK
     GTLSFSGIPD IRDTLMRLEI GASLGAGELL KISSVLTATL RAKNYGYNQK NNEETEEAAQ
     DTLTERFHLL EPLSPINNEI RRCIISEEEI ADDASPGLKS VRRQIKITND KIHESLGSIL
     NSASTKGMLQ DAIITMRNGR YCLPIKQEYK NTFQGMMHDQ SSTGSTAFIE PMAIVKLNNE
     LAELAVREQE EIEKILAELS NLVATEKYNL KYNQTTLAEL DFIFARAGLS KNMKASQPHF
     NNRHYINIKK GRHPLIDPKK VVPIDIYFGD KFDLLVITGP NTGGKTVSLK TVGLFTLMGQ
     AGLHIPAFDG SELSIFEEVY ADIGDEQSIE QSLSTFSSHM TNTVSILEHA NENSLVLFDE
     LGAGTDPTEG AALAMAILSY LHQRKIRTMA TTHYSELKIF ALSTDGVSNA CCEFSVETLQ
     PTYRLLIGIP GKSNAFAISS KLGLSNYIIE KAREFIGTKD ESFEDVISNL EASRIAMEKD
     KAEAEQYKKE VEELKRKLAE KNSKIDDAKD RILREANEKA RTILQEAKDY ADETIRKYNK
     WGAGGANNKE MENERAALRE KLGDTDSSLV SKAKKNRKQH KPSDFKVGDS VHVISLNLKG
     SVSTLPNAKG DLYVQMGILR SLVNISDLEL IDEETIVAKA LTKTQSGKIR MSKSMSISPE
     LNIIGKRVDE ALPLVDKYLD DAYLAHLPQV TIIHGRGTGA LKEAVHAHLK RTNYVKGYRV
     GGFGEGDHGV TIVEFK