ID   MUTS2_PYRFU             Reviewed;         562 AA.
AC   Q8U3J2;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=DNA-binding protein MutS2 {ECO:0000255|HAMAP-Rule:MF_00971};
GN   Name=mutS2 {ECO:0000255|HAMAP-Rule:MF_00971}; OrderedLocusNames=PF0474;
OS   Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=186497;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX   PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA   Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA   DiRuggiero J., Robb F.T.;
RT   "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT   horikoshii inferred from complete genomic sequences.";
RL   Genetics 152:1299-1305(1999).
RN   [2]
RP   FUNCTION, ATPASE ACTIVITY, DNA-BINDING, COFACTOR, BIOPHYSICOCHEMICAL
RP   PROPERTIES, AND SUBUNIT.
RX   PubMed=11821933; DOI=10.1007/s00284-001-0025-5;
RA   Vijayvargia R., Biswas I.;
RT   "MutS2 family protein from Pyrococcus furiosus.";
RL   Curr. Microbiol. 44:224-228(2002).
CC   -!- FUNCTION: Has ATPase and non-specific DNA-binding activities. May be
CC       involved in recombination and/or recombinational repair. Not involved
CC       in mismatch repair. {ECO:0000255|HAMAP-Rule:MF_00971,
CC       ECO:0000269|PubMed:11821933}.
CC   -!- COFACTOR:
CC       Name=Co(2+); Xref=ChEBI:CHEBI:48828;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00971,
CC         ECO:0000269|PubMed:11821933};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00971,
CC         ECO:0000269|PubMed:11821933};
CC       Note=Divalent metal cation. Highest activity with cobalt or manganese.
CC       {ECO:0000255|HAMAP-Rule:MF_00971, ECO:0000269|PubMed:11821933};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Temperature dependence:
CC         Optimum temperature is 90 degrees Celsius for ATPase and DNA-binding
CC         activities. {ECO:0000269|PubMed:11821933};
CC   -!- SUBUNIT: Multimer. {ECO:0000269|PubMed:11821933}.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. Archaeal
CC       Muts2 subfamily. {ECO:0000255|HAMAP-Rule:MF_00971}.
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DR   EMBL; AE009950; AAL80598.1; -; Genomic_DNA.
DR   RefSeq; WP_011011591.1; NZ_CP023154.1.
DR   AlphaFoldDB; Q8U3J2; -.
DR   SMR; Q8U3J2; -.
DR   STRING; 186497.PF0474; -.
DR   EnsemblBacteria; AAL80598; AAL80598; PF0474.
DR   GeneID; 41712275; -.
DR   KEGG; pfu:PF0474; -.
DR   PATRIC; fig|186497.12.peg.498; -.
DR   eggNOG; arCOG02895; Archaea.
DR   HOGENOM; CLU_026764_0_0_2; -.
DR   OMA; HYHAKTQ; -.
DR   OrthoDB; 9037at2157; -.
DR   PhylomeDB; Q8U3J2; -.
DR   Proteomes; UP000001013; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00971; MutS2_archaea; 1.
DR   InterPro; IPR012401; DNA-bd_MutS2_arc.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR045076; MutS_family.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR11361; PTHR11361; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   PIRSF; PIRSF029254; MutS_C_archaeal; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; DNA-binding; Hydrolase; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..562
FT                   /note="DNA-binding protein MutS2"
FT                   /id="PRO_0000423826"
FT   BINDING         380..387
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00971"
SQ   SEQUENCE   562 AA;  64826 MW;  46A55039D1B70040 CRC64;
     MKLRGDAREV YKRLLSRLES MIKLGEARTF LKKFEPTSDR EEIIKRQNYL KEGLKNVRDD
     LEEYLLSIRP IRFRREFFHD RILLVSDEEV EEAEKLDLCP VTSDPSEIED YPLILSTIGY
     GIEVEVKPSH IAPELYIIPL WENRDVLEAL SKVFPGGAAD KILISLKEIE EIFKKMEILE
     NLDEIIVEKE KELNRKIEEK LERFKLTLSG RDLVEFMKAL RAGNLEYLFH KFSALNDEII
     EEINKAEKEI SDVLGISVEI FPRDFPVEVP PEQIEALKRE LEREFKIEFY LKSRETVEKI
     LPHLQKLKEE IQKAYELYFL LVVKKFTRDF VFPEIVEEGI GFIEGRNLFI ENPQPVSYFV
     GKSYGNFPGV EEANIVILTG ANSGGKTSLL ELISQIVILA HMGFPVPAKK AWFTVLDEIF
     FFKRKRSVYG AGAFETSLKG LVRAIKGKGK KLILIDEFES ITEPGAAAKI LAELLKIAYE
     KGFFVVIVSH LGEDLKREIP FARVDGIEAK GLDENLNLIV DRQPKFGVIG RSTPELIVER
     LARKGRGEEK MIMNRILKKF RK