ID   MUTS2_RUMCH             Reviewed;         792 AA.
AC   B8I1Z8;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Endonuclease MutS2 {ECO:0000255|HAMAP-Rule:MF_00092};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00092};
GN   Name=mutS2 {ECO:0000255|HAMAP-Rule:MF_00092}; OrderedLocusNames=Ccel_1470;
OS   Ruminiclostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 /
OS   H10) (Clostridium cellulolyticum).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC   Ruminiclostridium.
OX   NCBI_TaxID=394503;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35319 / DSM 5812 / JCM 6584 / H10;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Chertkov O., Saunders E., Brettin T.,
RA   Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Ivanova N., Zhou J., Richardson P.;
RT   "Complete sequence of Clostridium cellulolyticum H10.";
RL   Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Endonuclease that is involved in the suppression of
CC       homologous recombination and may therefore have a key role in the
CC       control of bacterial genetic diversity. {ECO:0000255|HAMAP-
CC       Rule:MF_00092}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00092}.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MutS2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00092}.
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DR   EMBL; CP001348; ACL75824.1; -; Genomic_DNA.
DR   RefSeq; WP_015924966.1; NC_011898.1.
DR   AlphaFoldDB; B8I1Z8; -.
DR   SMR; B8I1Z8; -.
DR   STRING; 394503.Ccel_1470; -.
DR   PRIDE; B8I1Z8; -.
DR   EnsemblBacteria; ACL75824; ACL75824; Ccel_1470.
DR   KEGG; cce:Ccel_1470; -.
DR   eggNOG; COG1193; Bacteria.
DR   HOGENOM; CLU_011252_2_1_9; -.
DR   OMA; IHAIIND; -.
DR   OrthoDB; 256392at2; -.
DR   Proteomes; UP000001349; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   GO; GO:0045910; P:negative regulation of DNA recombination; IEA:InterPro.
DR   CDD; cd03280; ABC_MutS2; 1.
DR   Gene3D; 3.30.1370.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00092; MutS2; 1.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR   InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR   InterPro; IPR005747; MutS2.
DR   InterPro; IPR045076; MutS_family.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002625; Smr_dom.
DR   InterPro; IPR036063; Smr_dom_sf.
DR   PANTHER; PTHR11361; PTHR11361; 1.
DR   PANTHER; PTHR11361:SF14; PTHR11361:SF14; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   Pfam; PF01713; Smr; 1.
DR   PIRSF; PIRSF005814; MutS_YshD; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SMART; SM00533; MUTSd; 1.
DR   SMART; SM00463; SMR; 1.
DR   SUPFAM; SSF160443; SSF160443; 1.
DR   SUPFAM; SSF48334; SSF48334; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01069; mutS2; 1.
DR   PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
DR   PROSITE; PS50828; SMR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..792
FT                   /note="Endonuclease MutS2"
FT                   /id="PRO_1000192216"
FT   DOMAIN          717..792
FT                   /note="Smr"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
FT   BINDING         334..341
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
SQ   SEQUENCE   792 AA;  87948 MW;  7CA32124F38B68D2 CRC64;
     MNEKTHRVLE FDKILDKLKG LTASELGREL VLELTPQTDY RVVEKMLSET NDGVSCIMRR
     GSPPLGGITD IRMSLKRLDM GGVLNPGELL RLAGVLRAAR RLKGYINDKL DENNASVVKE
     LISCLESNQR LEQKIDNCIL SEDEIADNAS PALSSIRRQI KEQQASIKDK LNSIIRSTKY
     QKYIQESVVT MRGDRYVIPV KQEHKGDIPG LVHDSSASGA TLFIEPMAVV EANNSIKQLR
     VKEQTEIDRI LAELSQDASL VLPQLNANMS IMARLDFIFA KSKLAIDYNC ICPKINDTGK
     IIIKKGRHPL LDPKIVVPID FWIGEKFSSL IVTGPNTGGK TVSLKTVGLF TLMMQSGLLV
     PANDGTEMSV FEKIYADIGD EQSIEQSLST FSSHMKNIVD ILSGVNNKSL ILLDELGAGT
     DPTEGAALAM SILECLHQMG ATTLATTHYS ELKVYAISTT GVENASCEFD VETLRPTYRL
     LIGVPGKSNA FAISKRLGLT DDIIERSKEF LSQEDIRFED ILLSIEKNRS EAEKEKMRAE
     SYRQEAERLK KDLEDQKRRL AAQKESELRK AREEARRILT DSKRQADELV SEMKRLAKEQ
     EEAEVRRQTE ELRQKLNKSI NNLDDSLVES IMPRQGLVKP PKNLKPGDTV LIVNLNQKGT
     VLTLPDKNGE AQVQAGIMKI NVHISNLKLV DEQKQQIQRT GMGKIGVSKA QNMSTEIDLR
     GMMLSEAVDV VDKYLDDASI AGMGGVTLIH GKGTGALRAG LHQHLKHNPH IKSFRLGKLG
     EGENGVTVVE LK