A13_VACCW
ID A13_VACCW Reviewed; 70 AA.
AC Q76ZQ4; Q711L9;
DT 14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 23-FEB-2022, entry version 40.
DE RecName: Full=Virion membrane protein A13;
DE AltName: Full=Protein P8;
GN Name=VACWR132; OrderedLocusNames=A13L;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Pulford D.J., Meyer H., Ulaeto D.;
RT "Orthologs of the vaccinia virus A13L and A36R virion surface protein genes
RT display diversity in species of the genus Orthopoxvirus.";
RL Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Elstree;
RA Pulford D.J., Meyer H., Ulaeto D.;
RT "Orthologs of the vaccinia virus A13L and A36R virion surface protein genes
RT display diversity in the species of the genus Orthopoxvirus.";
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=8892867; DOI=10.1128/jvi.70.11.7485-7497.1996;
RA Jensen O.N., Houthaeve T., Shevchenko A., Cudmore S., Ashford T., Mann M.,
RA Griffiths G., Krijnse Locker J.;
RT "Identification of the major membrane and core proteins of vaccinia virus
RT by two-dimensional electrophoresis.";
RL J. Virol. 70:7485-7497(1996).
RN [5]
RP PHOSPHORYLATION AT SER-40, INDUCTION, AND MUTAGENESIS OF SER-21; SER-29;
RP SER-40; SER-60; SER-68 AND SER-69.
RX PubMed=15280497; DOI=10.1128/jvi.78.16.8885-8901.2004;
RA Unger B., Traktman P.;
RT "Vaccinia virus morphogenesis: a13 phosphoprotein is required for assembly
RT of mature virions.";
RL J. Virol. 78:8885-8901(2004).
CC -!- FUNCTION: Essential for the encapsidation of DNA into immature virions
CC (IV) and the subsequent maturation of IV into mature virions (MV).
CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000269|PubMed:8892867};
CC Single-pass membrane protein {ECO:0000269|PubMed:8892867}.
CC Note=Component of the mature virion (MV) membrane. The mature virion is
CC located in the cytoplasm of infected cells and is probably released by
CC cell lysis (Probable). {ECO:0000305}.
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC {ECO:0000269|PubMed:15280497}.
CC -!- PTM: Phosphorylated by a F10-independent mechanism.
CC {ECO:0000269|PubMed:15280497}.
CC -!- SIMILARITY: Belongs to the chordopoxvirinae A13 family. {ECO:0000305}.
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DR EMBL; AY243312; AAO89411.1; -; Genomic_DNA.
DR EMBL; AJ314915; CAC42825.1; -; Genomic_DNA.
DR EMBL; AJ309902; CAC85933.1; -; Genomic_DNA.
DR RefSeq; YP_233014.1; NC_006998.1.
DR SMR; Q76ZQ4; -.
DR iPTMnet; Q76ZQ4; -.
DR ABCD; Q76ZQ4; 1 sequenced antibody.
DR DNASU; 3707530; -.
DR GeneID; 3707530; -.
DR KEGG; vg:3707530; -.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR009236; Chordopox_A13L.
DR Pfam; PF05961; Chordopox_A13L; 1.
PE 1: Evidence at protein level;
KW Late protein; Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Virion.
FT CHAIN 1..70
FT /note="Virion membrane protein A13"
FT /id="PRO_0000414114"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 22..70
FT /note="Virion surface"
FT /evidence="ECO:0000255"
FT REGION 30..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 40
FT /note="Phosphoserine; by host"
FT /evidence="ECO:0000305|PubMed:15280497"
FT MUTAGEN 21
FT /note="S->A: No effect on phosphorylation."
FT /evidence="ECO:0000269|PubMed:15280497"
FT MUTAGEN 29
FT /note="S->A: No effect on phosphorylation."
FT /evidence="ECO:0000269|PubMed:15280497"
FT MUTAGEN 40
FT /note="S->A: Complete loss of phosphorylation."
FT /evidence="ECO:0000269|PubMed:15280497"
FT MUTAGEN 60
FT /note="S->A: No effect on phosphorylation."
FT /evidence="ECO:0000269|PubMed:15280497"
FT MUTAGEN 61
FT /note="S->A: No effect on phosphorylation."
FT MUTAGEN 68
FT /note="S->A: No effect on phosphorylation."
FT /evidence="ECO:0000269|PubMed:15280497"
FT MUTAGEN 69
FT /note="S->A: No effect on phosphorylation."
FT /evidence="ECO:0000269|PubMed:15280497"
SQ SEQUENCE 70 AA; 7696 MW; DB2F50B6E75F7955 CRC64;
MIGILLLIGI CVAVTVAILY SMYNKIKNSQ NPNPSPNLNS PPPEPKNTKF VNNLEKDHIS
SLYNLVKSSV
