ID   MUTS2_THENN             Reviewed;         757 AA.
AC   B9K937;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Endonuclease MutS2 {ECO:0000255|HAMAP-Rule:MF_00092};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00092};
GN   Name=mutS2 {ECO:0000255|HAMAP-Rule:MF_00092}; OrderedLocusNames=CTN_1294;
OS   Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E).
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX   NCBI_TaxID=309803;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E;
RA   Lim S.K., Kim J.S., Cha S.H., Park B.C., Lee D.S., Tae H.S., Kim S.-J.,
RA   Kim J.J., Park K.J., Lee S.Y.;
RT   "The genome sequence of the hyperthermophilic bacterium Thermotoga
RT   neapolitana.";
RL   Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Endonuclease that is involved in the suppression of
CC       homologous recombination and may therefore have a key role in the
CC       control of bacterial genetic diversity. {ECO:0000255|HAMAP-
CC       Rule:MF_00092}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00092}.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family. MutS2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00092}.
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DR   EMBL; CP000916; ACM23470.1; -; Genomic_DNA.
DR   RefSeq; WP_015919769.1; NC_011978.1.
DR   AlphaFoldDB; B9K937; -.
DR   SMR; B9K937; -.
DR   STRING; 309803.CTN_1294; -.
DR   EnsemblBacteria; ACM23470; ACM23470; CTN_1294.
DR   KEGG; tna:CTN_1294; -.
DR   eggNOG; COG1193; Bacteria.
DR   HOGENOM; CLU_011252_2_1_0; -.
DR   OMA; IHAIIND; -.
DR   Proteomes; UP000000445; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0006298; P:mismatch repair; IEA:InterPro.
DR   GO; GO:0045910; P:negative regulation of DNA recombination; IEA:InterPro.
DR   CDD; cd03280; ABC_MutS2; 1.
DR   Gene3D; 3.30.1370.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00092; MutS2; 1.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR   InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR   InterPro; IPR005747; MutS2.
DR   InterPro; IPR045076; MutS_family.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002625; Smr_dom.
DR   InterPro; IPR036063; Smr_dom_sf.
DR   PANTHER; PTHR11361; PTHR11361; 1.
DR   PANTHER; PTHR11361:SF14; PTHR11361:SF14; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   Pfam; PF01713; Smr; 1.
DR   PIRSF; PIRSF005814; MutS_YshD; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SMART; SM00533; MUTSd; 1.
DR   SMART; SM00463; SMR; 1.
DR   SUPFAM; SSF160443; SSF160443; 1.
DR   SUPFAM; SSF48334; SSF48334; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01069; mutS2; 1.
DR   PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
DR   PROSITE; PS50828; SMR; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Nucleotide-binding.
FT   CHAIN           1..757
FT                   /note="Endonuclease MutS2"
FT                   /id="PRO_1000118573"
FT   DOMAIN          681..756
FT                   /note="Smr"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
FT   BINDING         321..328
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00092"
SQ   SEQUENCE   757 AA;  86259 MW;  27F5588A4E5B9728 CRC64;
     MDYLEVLDFP KVVDLVKRHT FSDLGKRHLD TLKPTVNPWN ELELVEELLN YLTRWGEPPI
     KGLSDITPEM EKLKAGSSLE PWELLRVSSF LEGCDILKND LTRREYQKLK ETFSQLVSFE
     EFVKEVNRCI EQNGEVSNRA SPKLKEIRSE KRSLSTEIKK RADDFVKNHS QILQEQMYVY
     RDGRYLFPVK ASMKRSVRGI VHHLSSSGAT VFMEPEEFVE LNNRMRLLEE EERLEISRIL
     RHLTNMLLSN LKDLEKNIDL IAHFDSLYAR AKFAKENNGI VVKPSSRIKL VNARHPLIPK
     DRVVPINLEL PPNKKGVIIT GPNMGGKTVT VKTVGLFTAL MMSGFPLLCD EGTELKIFPK
     IMADIGEEQS IEQSLSTFSS HMKRIVEIVR NADSDSLVIL DELGSGTDPV EGAALAIAII
     EDLLEKGATL FVTTHLTPVK VFAMNHPLLL NASMEFDPET LSPTYRVLVG VPGGSHAFQI
     AEKLGLEKRI IENARSRLSQ EEMELEGLIR SLHEKISLLE EEKRKLQKEK EEYMKLRAKY
     EEDYKKLRRM KIEEFDKELK ELNDYIRKVK KELDQAIHVA KSGSVEEMRK TVKTLEKERE
     DLKKKAIEEE AEEEINVGDH VRMEGGTSVG KVVEVKGNTA LVDFGFLRVK VPLGKLKKAK
     KKEEEDREKG THFVSSFRTE IDIRGMTVEE AEPVVKKFID DLVMNGIKKG YIIHGKGTGK
     LATGVWEILR KDRRVVSFRF GTPSEGGTGV TVVEVEV