MUTS_ECOLI
ID MUTS_ECOLI Reviewed; 853 AA.
AC P23909; P71279; Q2MA96;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-1992, sequence version 1.
DT 03-AUG-2022, entry version 182.
DE RecName: Full=DNA mismatch repair protein MutS;
GN Name=mutS; Synonyms=fdv; OrderedLocusNames=b2733, JW2703;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1938937; DOI=10.1128/jb.173.23.7414-7415.1991;
RA Schlensog V., Boeck A.;
RT "The Escherichia coli fdv gene probably encodes mutS and is located at
RT minute 58.8 adjacent to the hyc-hyp gene cluster.";
RL J. Bacteriol. 173:7414-7415(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 602-731.
RC STRAIN=Various strains;
RA Denamur E.;
RL Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY 2D-GEL.
RX PubMed=9298644; DOI=10.1002/elps.1150180805;
RA VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT "Escherichia coli proteome analysis using the gene-protein database.";
RL Electrophoresis 18:1243-1251(1997).
CC -!- FUNCTION: This protein is involved in the repair of mismatches in DNA.
CC It is possible that it carries out the mismatch recognition step. This
CC protein has a weak ATPase activity.
CC -!- INTERACTION:
CC P23909; P23367: mutL; NbExp=4; IntAct=EBI-554920, EBI-554913;
CC P23909; P23909: mutS; NbExp=4; IntAct=EBI-554920, EBI-554920;
CC P23909; P0CE47: tufA; NbExp=2; IntAct=EBI-554920, EBI-301077;
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
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DR EMBL; M64730; AAA24188.1; -; Genomic_DNA.
DR EMBL; U29579; AAA69243.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75775.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76810.1; -; Genomic_DNA.
DR EMBL; AF004287; AAD01197.1; -; Genomic_DNA.
DR EMBL; AF001987; AAD00921.1; -; Genomic_DNA.
DR EMBL; AF001988; AAD00922.1; -; Genomic_DNA.
DR EMBL; AF001989; AAD00923.1; -; Genomic_DNA.
DR EMBL; AF001990; AAD00924.1; -; Genomic_DNA.
DR EMBL; AF001991; AAD00925.1; -; Genomic_DNA.
DR EMBL; AF001992; AAD00926.1; -; Genomic_DNA.
DR EMBL; AF001993; AAD00927.1; -; Genomic_DNA.
DR EMBL; AF001994; AAD00928.1; -; Genomic_DNA.
DR EMBL; AF001995; AAD00929.1; -; Genomic_DNA.
DR EMBL; AF001996; AAD00930.1; -; Genomic_DNA.
DR EMBL; AF001997; AAD00931.1; -; Genomic_DNA.
DR EMBL; AF001998; AAD00932.1; -; Genomic_DNA.
DR EMBL; AF001999; AAD00933.1; -; Genomic_DNA.
DR EMBL; AF002000; AAD00934.1; -; Genomic_DNA.
DR EMBL; AF002001; AAD00935.1; -; Genomic_DNA.
DR EMBL; AF002002; AAD00936.1; -; Genomic_DNA.
DR EMBL; AF002003; AAD00937.1; -; Genomic_DNA.
DR EMBL; AF002004; AAD00938.1; -; Genomic_DNA.
DR EMBL; AF002005; AAD00939.1; -; Genomic_DNA.
DR EMBL; AF002006; AAD00940.1; -; Genomic_DNA.
DR EMBL; AF002007; AAD00941.1; -; Genomic_DNA.
DR EMBL; AF002008; AAD00942.1; -; Genomic_DNA.
DR EMBL; AF002009; AAD00943.1; -; Genomic_DNA.
DR EMBL; AF002010; AAD00944.1; -; Genomic_DNA.
DR PIR; I54964; I54964.
DR RefSeq; NP_417213.1; NC_000913.3.
DR RefSeq; WP_001272928.1; NZ_LN832404.1.
DR PDB; 1E3M; X-ray; 2.20 A; A/B=1-800.
DR PDB; 1NG9; X-ray; 2.60 A; A/B=1-800.
DR PDB; 1OH5; X-ray; 2.90 A; A/B=1-800.
DR PDB; 1OH6; X-ray; 2.40 A; A/B=1-800.
DR PDB; 1OH7; X-ray; 2.50 A; A/B=1-800.
DR PDB; 1OH8; X-ray; 2.90 A; A/B=1-800.
DR PDB; 1W7A; X-ray; 2.27 A; A/B=1-800.
DR PDB; 1WB9; X-ray; 2.10 A; A/B=1-800.
DR PDB; 1WBB; X-ray; 2.50 A; A/B=1-800.
DR PDB; 1WBD; X-ray; 2.40 A; A/B=1-800.
DR PDB; 2OK2; X-ray; 2.00 A; A/B=820-853.
DR PDB; 2WTU; X-ray; 3.40 A; A/B=1-800.
DR PDB; 3K0S; X-ray; 2.20 A; A/B=2-800.
DR PDB; 3ZLJ; X-ray; 3.10 A; A/B=1-800, C/D=801-853.
DR PDB; 5AKB; X-ray; 4.71 A; A/B/E=1-800.
DR PDB; 5AKC; X-ray; 6.60 A; A/B/E/F/I/J=1-800.
DR PDB; 5AKD; X-ray; 7.60 A; A/B/E/F/I/J=1-800.
DR PDB; 6I5F; X-ray; 2.60 A; A/B=1-853.
DR PDB; 7AI5; EM; 5.00 A; A/B=1-853.
DR PDB; 7AI6; EM; 6.90 A; A/B=1-853.
DR PDB; 7AI7; EM; 3.90 A; A/B=1-853.
DR PDB; 7AIB; EM; 4.70 A; A/B=1-853.
DR PDB; 7AIC; EM; 5.00 A; A/B=1-853.
DR PDB; 7OTO; EM; 3.40 A; A/B=1-800.
DR PDB; 7OU0; EM; 3.80 A; A/B=1-799.
DR PDB; 7OU4; EM; 3.20 A; A/B=1-800.
DR PDBsum; 1E3M; -.
DR PDBsum; 1NG9; -.
DR PDBsum; 1OH5; -.
DR PDBsum; 1OH6; -.
DR PDBsum; 1OH7; -.
DR PDBsum; 1OH8; -.
DR PDBsum; 1W7A; -.
DR PDBsum; 1WB9; -.
DR PDBsum; 1WBB; -.
DR PDBsum; 1WBD; -.
DR PDBsum; 2OK2; -.
DR PDBsum; 2WTU; -.
DR PDBsum; 3K0S; -.
DR PDBsum; 3ZLJ; -.
DR PDBsum; 5AKB; -.
DR PDBsum; 5AKC; -.
DR PDBsum; 5AKD; -.
DR PDBsum; 6I5F; -.
DR PDBsum; 7AI5; -.
DR PDBsum; 7AI6; -.
DR PDBsum; 7AI7; -.
DR PDBsum; 7AIB; -.
DR PDBsum; 7AIC; -.
DR PDBsum; 7OTO; -.
DR PDBsum; 7OU0; -.
DR PDBsum; 7OU4; -.
DR AlphaFoldDB; P23909; -.
DR SASBDB; P23909; -.
DR SMR; P23909; -.
DR BioGRID; 4261421; 143.
DR ComplexPortal; CPX-5541; MutHLS methyl-directed mismatch repair complex.
DR ComplexPortal; CPX-93; MutS DNA mismatch repair complex.
DR DIP; DIP-10287N; -.
DR IntAct; P23909; 10.
DR MINT; P23909; -.
DR STRING; 511145.b2733; -.
DR SWISS-2DPAGE; P23909; -.
DR jPOST; P23909; -.
DR PaxDb; P23909; -.
DR PRIDE; P23909; -.
DR EnsemblBacteria; AAC75775; AAC75775; b2733.
DR EnsemblBacteria; BAE76810; BAE76810; BAE76810.
DR GeneID; 947206; -.
DR KEGG; ecj:JW2703; -.
DR KEGG; eco:b2733; -.
DR PATRIC; fig|1411691.4.peg.4007; -.
DR EchoBASE; EB0620; -.
DR eggNOG; COG0249; Bacteria.
DR HOGENOM; CLU_002472_4_0_6; -.
DR InParanoid; P23909; -.
DR OMA; TPMMAQY; -.
DR PhylomeDB; P23909; -.
DR BioCyc; EcoCyc:EG10625-MON; -.
DR BioCyc; MetaCyc:EG10625-MON; -.
DR EvolutionaryTrace; P23909; -.
DR PRO; PR:P23909; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0032300; C:mismatch repair complex; IC:ComplexPortal.
DR GO; GO:1990710; C:MutS complex; IPI:ComplexPortal.
DR GO; GO:0032136; F:adenine/cytosine mispair binding; IDA:EcoliWiki.
DR GO; GO:0043531; F:ADP binding; IDA:EcoliWiki.
DR GO; GO:0005524; F:ATP binding; IDA:EcoliWiki.
DR GO; GO:0016887; F:ATP hydrolysis activity; IDA:EcoliWiki.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003684; F:damaged DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008301; F:DNA binding, bending; IDA:EcoliWiki.
DR GO; GO:0003690; F:double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0030983; F:mismatched DNA binding; IDA:EcoliWiki.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR GO; GO:0006298; P:mismatch repair; IDA:EcoliWiki.
DR GO; GO:0000018; P:regulation of DNA recombination; IMP:EcoliWiki.
DR Gene3D; 3.30.420.110; -; 1.
DR Gene3D; 3.40.1170.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00096; MutS; 1.
DR InterPro; IPR005748; DNA_mismatch_repair_MutS.
DR InterPro; IPR007695; DNA_mismatch_repair_MutS-lik_N.
DR InterPro; IPR017261; DNA_mismatch_repair_MutS/MSH.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007861; DNA_mismatch_repair_MutS_clamp.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR016151; DNA_mismatch_repair_MutS_N.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR007860; DNA_mmatch_repair_MutS_con_dom.
DR InterPro; IPR036678; MutS_con_dom_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF01624; MutS_I; 1.
DR Pfam; PF05188; MutS_II; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF05190; MutS_IV; 1.
DR Pfam; PF00488; MutS_V; 1.
DR PIRSF; PIRSF037677; DNA_mis_repair_Msh6; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF53150; SSF53150; 1.
DR SUPFAM; SSF55271; SSF55271; 1.
DR TIGRFAMs; TIGR01070; mutS1; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; DNA damage; DNA repair; DNA-binding;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..853
FT /note="DNA mismatch repair protein MutS"
FT /id="PRO_0000115094"
FT BINDING 614..621
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT HELIX 7..9
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 12..23
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 27..33
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 36..40
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 41..51
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 56..58
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 61..63
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 66..72
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 73..75
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 76..85
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 90..95
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 99..101
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 103..105
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 108..114
FT /evidence="ECO:0007829|PDB:1WB9"
FT TURN 116..118
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 122..124
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 133..138
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 143..148
FT /evidence="ECO:0007829|PDB:1WB9"
FT TURN 150..152
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 155..158
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 163..173
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 176..181
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 187..189
FT /evidence="ECO:0007829|PDB:1WB9"
FT TURN 190..192
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 194..199
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 201..204
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 206..217
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 220..222
FT /evidence="ECO:0007829|PDB:6I5F"
FT HELIX 223..225
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 231..248
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 249..251
FT /evidence="ECO:0007829|PDB:7OTO"
FT HELIX 252..254
FT /evidence="ECO:0007829|PDB:7OTO"
FT STRAND 258..260
FT /evidence="ECO:0007829|PDB:6I5F"
FT HELIX 263..265
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 271..276
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 279..281
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 287..290
FT /evidence="ECO:0007829|PDB:1W7A"
FT HELIX 291..295
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 301..312
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 318..330
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 332..334
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 335..343
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 348..356
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 362..372
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 375..383
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 388..397
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 401..410
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 419..421
FT /evidence="ECO:0007829|PDB:7OU4"
FT HELIX 431..458
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 464..468
FT /evidence="ECO:0007829|PDB:1WB9"
FT TURN 469..471
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 472..478
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 479..482
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 490..494
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 496..502
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 504..535
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 536..538
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 539..564
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 570..572
FT /evidence="ECO:0007829|PDB:1OH7"
FT STRAND 574..576
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 578..583
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 588..591
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 592..594
FT /evidence="ECO:0007829|PDB:1W7A"
FT STRAND 599..603
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 605..607
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 609..613
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 620..634
FT /evidence="ECO:0007829|PDB:1WB9"
FT TURN 635..637
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 640..647
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 652..657
FT /evidence="ECO:0007829|PDB:1WB9"
FT TURN 663..666
FT /evidence="ECO:0007829|PDB:7OU4"
FT HELIX 671..683
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 688..694
FT /evidence="ECO:0007829|PDB:1WB9"
FT TURN 695..698
FT /evidence="ECO:0007829|PDB:6I5F"
FT STRAND 699..702
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 703..717
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 722..726
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 730..734
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 735..738
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 742..752
FT /evidence="ECO:0007829|PDB:1WB9"
FT STRAND 755..765
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 772..778
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 783..797
FT /evidence="ECO:0007829|PDB:1WB9"
FT HELIX 826..831
FT /evidence="ECO:0007829|PDB:3ZLJ"
FT TURN 834..836
FT /evidence="ECO:0007829|PDB:3ZLJ"
FT HELIX 839..852
FT /evidence="ECO:0007829|PDB:3ZLJ"
SQ SEQUENCE 853 AA; 95247 MW; 5A1F295362671D55 CRC64;
MSAIENFDAH TPMMQQYLRL KAQHPEILLF YRMGDFYELF YDDAKRASQL LDISLTKRGA
SAGEPIPMAG IPYHAVENYL AKLVNQGESV AICEQIGDPA TSKGPVERKV VRIVTPGTIS
DEALLQERQD NLLAAIWQDS KGFGYATLDI SSGRFRLSEP ADRETMAAEL QRTNPAELLY
AEDFAEMSLI EGRRGLRRRP LWEFEIDTAR QQLNLQFGTR DLVGFGVENA PRGLCAAGCL
LQYAKDTQRT TLPHIRSITM EREQDSIIMD AATRRNLEIT QNLAGGAENT LASVLDCTVT
PMGSRMLKRW LHMPVRDTRV LLERQQTIGA LQDFTAGLQP VLRQVGDLER ILARLALRTA
RPRDLARMRH AFQQLPELRA QLETVDSAPV QALREKMGEF AELRDLLERA IIDTPPVLVR
DGGVIASGYN EELDEWRALA DGATDYLERL EVRERERTGL DTLKVGFNAV HGYYIQISRG
QSHLAPINYM RRQTLKNAER YIIPELKEYE DKVLTSKGKA LALEKQLYEE LFDLLLPHLE
ALQQSASALA ELDVLVNLAE RAYTLNYTCP TFIDKPGIRI TEGRHPVVEQ VLNEPFIANP
LNLSPQRRML IITGPNMGGK STYMRQTALI ALMAYIGSYV PAQKVEIGPI DRIFTRVGAA
DDLASGRSTF MVEMTETANI LHNATEYSLV LMDEIGRGTS TYDGLSLAWA CAENLANKIK
ALTLFATHYF ELTQLPEKME GVANVHLDAL EHGDTIAFMH SVQDGAASKS YGLAVAALAG
VPKEVIKRAR QKLRELESIS PNAAATQVDG TQMSLLSVPE ETSPAVEALE NLDPDSLTPR
QALEWIYRLK SLV
