MUTS_THET8
ID MUTS_THET8 Reviewed; 819 AA.
AC Q56239; Q5SIP5;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=DNA mismatch repair protein MutS;
GN Name=mutS; OrderedLocusNames=TTHA1324;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21, AND
RP CHARACTERIZATION.
RX PubMed=8604304; DOI=10.1093/nar/24.4.640;
RA Takamatsu S., Kato R., Kuramitsu S.;
RT "Mismatch DNA recognition protein from an extremely thermophilic bacterium,
RT Thermus thermophilus HB8.";
RL Nucleic Acids Res. 24:640-647(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP DOMAIN STRUCTURE.
RX PubMed=9722634; DOI=10.1093/nar/26.18.4153;
RA Tachiki H., Kato R., Masui R., Hasegawa K., Itakura H., Fukuyama K.,
RA Kuramitsu S.;
RT "Domain organization and functional analysis of Thermus thermophilus MutS
RT protein.";
RL Nucleic Acids Res. 26:4153-4159(1998).
CC -!- FUNCTION: This protein is involved in the repair of mismatches in DNA.
CC It is possible that it carries out the mismatch recognition step. Binds
CC double-stranded DNA.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Temperature dependence:
CC Optimum temperature is 80 degrees Celsius for ATPase activity.;
CC -!- SUBUNIT: Homotetramer.
CC -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC {ECO:0000305}.
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DR EMBL; D63810; BAA09880.1; -; Genomic_DNA.
DR EMBL; AP008226; BAD71147.1; -; Genomic_DNA.
DR RefSeq; YP_144590.1; NC_006461.1.
DR AlphaFoldDB; Q56239; -.
DR SMR; Q56239; -.
DR STRING; 300852.55772706; -.
DR EnsemblBacteria; BAD71147; BAD71147; BAD71147.
DR KEGG; ttj:TTHA1324; -.
DR PATRIC; fig|300852.9.peg.1302; -.
DR eggNOG; COG0249; Bacteria.
DR HOGENOM; CLU_002472_4_0_0; -.
DR OMA; TPMMAQY; -.
DR PhylomeDB; Q56239; -.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR GO; GO:0003684; F:damaged DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR GO; GO:0006298; P:mismatch repair; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.420.110; -; 1.
DR Gene3D; 3.40.1170.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00096; MutS; 1.
DR InterPro; IPR005748; DNA_mismatch_repair_MutS.
DR InterPro; IPR007695; DNA_mismatch_repair_MutS-lik_N.
DR InterPro; IPR017261; DNA_mismatch_repair_MutS/MSH.
DR InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR InterPro; IPR007861; DNA_mismatch_repair_MutS_clamp.
DR InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR InterPro; IPR016151; DNA_mismatch_repair_MutS_N.
DR InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR InterPro; IPR007860; DNA_mmatch_repair_MutS_con_dom.
DR InterPro; IPR036678; MutS_con_dom_sf.
DR InterPro; IPR045076; MutS_family.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11361; PTHR11361; 1.
DR Pfam; PF01624; MutS_I; 1.
DR Pfam; PF05188; MutS_II; 1.
DR Pfam; PF05192; MutS_III; 1.
DR Pfam; PF05190; MutS_IV; 1.
DR Pfam; PF00488; MutS_V; 1.
DR PIRSF; PIRSF037677; DNA_mis_repair_Msh6; 1.
DR SMART; SM00534; MUTSac; 1.
DR SMART; SM00533; MUTSd; 1.
DR SUPFAM; SSF48334; SSF48334; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF53150; SSF53150; 1.
DR SUPFAM; SSF55271; SSF55271; 1.
DR TIGRFAMs; TIGR01070; mutS1; 1.
DR PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Direct protein sequencing; DNA damage; DNA repair;
KW DNA-binding; Nucleotide-binding; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:8604304"
FT CHAIN 2..819
FT /note="DNA mismatch repair protein MutS"
FT /id="PRO_0000115160"
FT REGION 2..130
FT /note="A1"
FT REGION 131..274
FT /note="A2"
FT REGION 275..570
FT /note="B; DNA-binding"
FT REGION 571..819
FT /note="C"
FT BINDING 591..598
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 789
FT /note="D -> N (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 819 AA; 91381 MW; DC033E736879863E CRC64;
MGGYGGVKME GMLKGEGPGP LPPLLQQYVE LRDRYPDYLL LFQVGDFYEC FGEDAERLAR
ALGLVLTHKT SKDFTTPMAG IPIRAFDAYA ERLLKMGFRL AVADQVEPAE EAEGLVRREV
TQLLTPGTLT QEALLPREAN YLAAIATGDG WGLAFLDVST GEFKGTLLKS KSALYDELFR
HRPAEVLLAP ELRENEAFVA EFRKRFPVML SEAPFEPQGE GPLALRRAQG ALLAYARATQ
GGALSVRPFR LYDPGAFVRL PEASLKALEV FEPLRGQDTL FGVLDETRTA PGRRLLQAWL
RHPLLERGPL EARLDRVERF VREGALREGV RRLLFRLADL ERLATRLELS RASPRDLAAL
RRSLEILPEL KGLLGEEVGL PDLSGLLEEL RAALVEDPPL KVSEGGLIRE GYDPDLDALR
RAHAEGVAYF LDLEAREKER TGIPTLKVGY NAVFGYYLEV TRPYYEKVPQ EYRPVQTLKD
RQRYTLPEMK ERERELYRLE ALIKRREEEV FLALRERARK EAEALREAAR ILAELDVYAA
LAEVAVRHGY TRPRFGERLR IRAGRHPVVE RRTAFVPNDL EMAHELVLVT GPNMAGKSTF
LRQTALIALL AQIGSFVPAE EAELPLFDGI YTRIGASDDL AGGKSTFMVE MEEVALVLKE
ATERSLVLLD EVGRGTSSLD GVAIATALAE ALHERRCYTL FATHYFELTA LALPRLKNLH
VAAKEEEGGL VFYHQVLPGP ASKSYGVEVA EMAGLPKEVV ERARALLSAM AARREGALEE
VLERLLALDP DRLTPLEALR FLHELKALAL GLPLGSMKG
