MVFR_PSEAE
ID MVFR_PSEAE Reviewed; 332 AA.
AC Q9I4X0;
DT 13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Multiple virulence factor regulator MvfR {ECO:0000303|PubMed:11724939};
DE AltName: Full=Transcriptional regulator MvfR;
GN Name=mvfR {ECO:0000303|PubMed:11724939}; OrderedLocusNames=PA1003;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11724939; DOI=10.1073/pnas.251465298;
RA Cao H., Krishnan G., Goumnerov B., Tsongalis J., Tompkins R., Rahme L.G.;
RT "A quorum sensing-associated virulence gene of Pseudomonas aeruginosa
RT encodes a LysR-like transcription regulator with a unique self-regulatory
RT mechanism.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:14613-14618(2001).
RN [3]
RP FUNCTION, AND ACTIVITY REGULATION.
RX PubMed=17083468; DOI=10.1111/j.1365-2958.2006.05462.x;
RA Xiao G., Deziel E., He J., Lepine F., Lesic B., Castonguay M.H., Milot S.,
RA Tampakaki A.P., Stachel S.E., Rahme L.G.;
RT "MvfR, a key Pseudomonas aeruginosa pathogenicity LTTR-class regulatory
RT protein, has dual ligands.";
RL Mol. Microbiol. 62:1689-1699(2006).
RN [4]
RP FUNCTION.
RX PubMed=27678057; DOI=10.1038/srep34083;
RA Maura D., Hazan R., Kitao T., Ballok A.E., Rahme L.G.;
RT "Evidence for direct control of virulence and defense gene circuits by the
RT Pseudomonas aeruginosa quorum sensing regulator, MvfR.";
RL Sci. Rep. 6:34083-34083(2016).
RN [5] {ECO:0007744|PDB:6B8A}
RP X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) OF 94-295, AND SUBUNIT.
RX PubMed=29339431; DOI=10.1128/mbio.02158-17;
RA Kitao T., Lepine F., Babloudi S., Walte F., Steinbacher S., Maskos K.,
RA Blaesse M., Negri M., Pucci M., Zahler B., Felici A., Rahme L.G.;
RT "Molecular Insights into Function and Competitive Inhibition of Pseudomonas
RT aeruginosa Multiple Virulence Factor Regulator.";
RL MBio 9:0-0(2018).
CC -!- FUNCTION: Transcription regulator that plays a critical role in
CC virulence by positively regulating the expression of multiple quorum
CC sensing (QS)-regulated virulence factors, genes involved in protein
CC secretion, translation, response to oxidative stress and the phnAB
CC operon (PubMed:11724939, PubMed:27678057, PubMed:17083468). At the
CC stationary phase, negatively autoregulates its function through
CC cleavage and translocation to the extracellular space
CC (PubMed:11724939). {ECO:0000269|PubMed:11724939,
CC ECO:0000269|PubMed:17083468, ECO:0000269|PubMed:27678057}.
CC -!- ACTIVITY REGULATION: Both 3,4-dihydroxy-2-heptylquinoline (PQS) and its
CC precursor 4-hydroxy-2-heptylquinoline (HHQ) function as ligands and
CC promote MvfR DNA-binding activity leading to transcriptional
CC activation. {ECO:0000269|PubMed:17083468}.
CC -!- SUBUNIT: Forms homooligomers. {ECO:0000269|PubMed:29339431}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:11724939}. Secreted {ECO:0000269|PubMed:11724939}.
CC Note=At stationary phase, gets cleaved and subsequently released in the
CC extracellular space where its transcriptional activity is inhibited.
CC {ECO:0000269|PubMed:11724939}.
CC -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC {ECO:0000305}.
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DR EMBL; AE004091; AAG04392.1; -; Genomic_DNA.
DR PIR; D83519; D83519.
DR RefSeq; NP_249694.1; NC_002516.2.
DR RefSeq; WP_003108614.1; NZ_QZGE01000006.1.
DR PDB; 6B8A; X-ray; 2.65 A; A/B=94-295.
DR PDB; 6Q7U; X-ray; 3.14 A; A=91-319.
DR PDB; 6Q7V; X-ray; 2.56 A; A/B=91-319.
DR PDB; 6Q7W; X-ray; 2.82 A; A=91-319.
DR PDB; 6YIZ; X-ray; 2.16 A; A/B=91-319.
DR PDB; 7NBW; X-ray; 2.28 A; A=91-319.
DR PDBsum; 6B8A; -.
DR PDBsum; 6Q7U; -.
DR PDBsum; 6Q7V; -.
DR PDBsum; 6Q7W; -.
DR PDBsum; 6YIZ; -.
DR PDBsum; 7NBW; -.
DR AlphaFoldDB; Q9I4X0; -.
DR SMR; Q9I4X0; -.
DR STRING; 287.DR97_945; -.
DR PaxDb; Q9I4X0; -.
DR DNASU; 879994; -.
DR EnsemblBacteria; AAG04392; AAG04392; PA1003.
DR GeneID; 879994; -.
DR KEGG; pae:PA1003; -.
DR PATRIC; fig|208964.12.peg.1035; -.
DR PseudoCAP; PA1003; -.
DR HOGENOM; CLU_060101_0_0_6; -.
DR OMA; QEDYCMF; -.
DR PhylomeDB; Q9I4X0; -.
DR BioCyc; PAER208964:G1FZ6-1022-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0051349; P:positive regulation of lyase activity; IMP:PseudoCAP.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0034762; P:regulation of transmembrane transport; IMP:PseudoCAP.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR005119; LysR_subst-bd.
DR InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00126; HTH_1; 1.
DR Pfam; PF03466; LysR_substrate; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50931; HTH_LYSR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; DNA-binding; Membrane;
KW Reference proteome; Secreted; Transcription; Transcription regulation;
KW Virulence.
FT CHAIN 1..332
FT /note="Multiple virulence factor regulator MvfR"
FT /id="PRO_0000448531"
FT DOMAIN 4..61
FT /note="HTH lysR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT DNA_BIND 21..40
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT HELIX 92..94
FT /evidence="ECO:0007829|PDB:6YIZ"
FT STRAND 95..100
FT /evidence="ECO:0007829|PDB:6YIZ"
FT HELIX 105..118
FT /evidence="ECO:0007829|PDB:6YIZ"
FT STRAND 122..127
FT /evidence="ECO:0007829|PDB:6YIZ"
FT HELIX 129..131
FT /evidence="ECO:0007829|PDB:6YIZ"
FT HELIX 132..137
FT /evidence="ECO:0007829|PDB:6YIZ"
FT HELIX 139..141
FT /evidence="ECO:0007829|PDB:6YIZ"
FT STRAND 145..149
FT /evidence="ECO:0007829|PDB:6YIZ"
FT STRAND 156..172
FT /evidence="ECO:0007829|PDB:6YIZ"
FT STRAND 174..176
FT /evidence="ECO:0007829|PDB:6B8A"
FT HELIX 177..180
FT /evidence="ECO:0007829|PDB:6YIZ"
FT STRAND 181..183
FT /evidence="ECO:0007829|PDB:6Q7V"
FT HELIX 186..190
FT /evidence="ECO:0007829|PDB:6YIZ"
FT STRAND 194..197
FT /evidence="ECO:0007829|PDB:6YIZ"
FT STRAND 200..202
FT /evidence="ECO:0007829|PDB:6Q7V"
FT TURN 206..208
FT /evidence="ECO:0007829|PDB:7NBW"
FT STRAND 213..220
FT /evidence="ECO:0007829|PDB:6YIZ"
FT HELIX 221..230
FT /evidence="ECO:0007829|PDB:6YIZ"
FT STRAND 234..238
FT /evidence="ECO:0007829|PDB:6YIZ"
FT HELIX 239..247
FT /evidence="ECO:0007829|PDB:6YIZ"
FT STRAND 249..254
FT /evidence="ECO:0007829|PDB:6YIZ"
FT TURN 256..258
FT /evidence="ECO:0007829|PDB:6YIZ"
FT STRAND 263..272
FT /evidence="ECO:0007829|PDB:6YIZ"
FT HELIX 273..277
FT /evidence="ECO:0007829|PDB:6YIZ"
FT HELIX 279..297
FT /evidence="ECO:0007829|PDB:6YIZ"
FT HELIX 298..300
FT /evidence="ECO:0007829|PDB:6YIZ"
SQ SEQUENCE 332 AA; 37214 MW; 117430C3C1232D97 CRC64;
MPIHNLNHVN MFLQVIASGS ISSAARILRK SHTAVSSAVS NLEIDLCVEL VRRDGYKVEP
TEQALRLIPY MRSLLNYQQL IGDIAFNLNK GPRNLRVLLD TAIPPSFCDT VSSVLLDDFN
MVSLIRTSPA DSLATIKQDN AEIDIAITID EELKISRFNQ CVLGYTKAFV VAHPQHPLCN
ASLHSIASLA NYRQISLGSR SGQHSNLLRP VSDKVLFVEN FDDMLRLVEA GVGWGIAPHY
FVEERLRNGT LAVLSELYEP GGIDTKVYCY YNTALESERS FLRFLESARQ RLRELGRQRF
DDAPAWQPSI VETAQRRSGP KALAYRQRAA PE
