MVHA_METTH
ID MVHA_METTH Reviewed; 472 AA.
AC Q50783; O27206;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-JAN-2004, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=F420-non-reducing hydrogenase subunit A;
DE EC=1.12.99.-;
DE AltName: Full=Methyl viologen-reducing hydrogenase subunit alpha;
DE Short=MVH subunit A;
GN Name=mvhA; OrderedLocusNames=MTH_1134;
OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=2654933; DOI=10.1073/pnas.86.9.3031;
RA Reeve J.N., Beckler G.S., Cram D.S., Hamilton P.T., Brown J.W.,
RA Krzycki J.A., Kolodziej A.F., Alex L., Orme-Johnson W.H., Walsh C.T.;
RT "A hydrogenase-linked gene in Methanobacterium thermoautotrophicum strain
RT delta H encodes a polyferredoxin.";
RL Proc. Natl. Acad. Sci. U.S.A. 86:3031-3035(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
CC -!- FUNCTION: Part of a complex that provides reducing equivalents for
CC heterodisulfide reductase. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Evidence={ECO:0000305};
CC -!- SUBUNIT: The F420-non-reducing hydrogenase is composed of three
CC subunits; MvhA, MvhD and MvhG. It forms a complex with the
CC heterodisulfide reductase (hdr) (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit
CC family. {ECO:0000305}.
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DR EMBL; J04540; AAB02351.1; -; Genomic_DNA.
DR EMBL; AE000666; AAB85623.1; -; Genomic_DNA.
DR PIR; A69018; A69018.
DR PIR; F30315; F30315.
DR RefSeq; WP_010876758.1; NC_000916.1.
DR AlphaFoldDB; Q50783; -.
DR SMR; Q50783; -.
DR IntAct; Q50783; 2.
DR STRING; 187420.MTH_1134; -.
DR EnsemblBacteria; AAB85623; AAB85623; MTH_1134.
DR GeneID; 1471542; -.
DR KEGG; mth:MTH_1134; -.
DR PATRIC; fig|187420.15.peg.1111; -.
DR HOGENOM; CLU_044556_0_0_2; -.
DR OMA; VQNNPAM; -.
DR BioCyc; MetaCyc:MVHAMAUTO-MON; -.
DR Proteomes; UP000005223; Chromosome.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR Gene3D; 1.10.645.10; -; 1.
DR InterPro; IPR001501; Ni-dep_hyd_lsu.
DR InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR InterPro; IPR029014; NiFe-Hase_large.
DR Pfam; PF00374; NiFeSe_Hases; 2.
DR SUPFAM; SSF56762; SSF56762; 1.
DR PROSITE; PS00507; NI_HGENASE_L_1; 1.
DR PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE 3: Inferred from homology;
KW Metal-binding; Nickel; Oxidoreductase; Reference proteome.
FT CHAIN 1..472
FT /note="F420-non-reducing hydrogenase subunit A"
FT /id="PRO_0000199726"
FT BINDING 61
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255"
FT BINDING 64
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255"
FT BINDING 442
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255"
FT BINDING 445
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000255"
FT CONFLICT 21
FT /note="H -> R (in Ref. 1; AAB02351)"
FT /evidence="ECO:0000305"
FT CONFLICT 267
FT /note="K -> T (in Ref. 1; AAB02351)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 472 AA; 52916 MW; 81A50D0D3156CD90 CRC64;
MVKLTMEPVT RIEGHAKITV HLDDAGNVED TRLHVMEFRG FEKFLQGRPI EEAPRIVPRI
CGICDVQHHL AAAKAVDACF GFEPEDVLPA AYKMREIMNW GSYMHSHGLH FYFLAAPDFI
AGKDRKTRNV FQIIKDAPDI ALQAIELRKN ALELVRATGG RPIHPTSSTP GGISTELDDE
TQKDLLKKAQ RNVELAEATL ELAVPIFEEN IDLVNSLGNI ETYHTGLVKD GVWDVYDGIV
RIKDKEGNMF REFKPADYAD TIAEHVKPYS WLKFPYIKDL GYPDGVYRVS PLSRLNVADK
MPDAAPKAQE HFKEFRENFG YAQQTLLYHW ARLIELLACA ECAADALEGD LSGEKFPDSL
ERQAGDGVGI VEAPRGTLTH HYTCDENGLI TKANIVVATI QNNPAMEMGI QKVAQDYIKP
GVEVDDKIFN LMEMVIRAYD PCLSCATHTI DSQMRLATLE VYDSEGDLVK RI
