MVPA_DICDI
ID MVPA_DICDI Reviewed; 843 AA.
AC P34118; Q55D21;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Major vault protein alpha;
DE Short=MVP-alpha;
GN Name=mvpA; ORFNames=DDB_G0269156;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 423-444.
RC STRAIN=AX4;
RX PubMed=8340365; DOI=10.1016/s0021-9258(18)82264-5;
RA Vasu S.K., Kedersha N.L., Rome L.H.;
RT "cDNA cloning and disruption of the major vault protein alpha gene (mvpA)
RT in Dictyostelium discoideum.";
RL J. Biol. Chem. 268:15356-15360(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP PROTEIN SEQUENCE OF 2-9; 28-37; 109-119; 164-174; 667-677; 749-757; 771-778
RP AND 795-810, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RA Bienvenut W.V., Patel H., Brunton V.G., Frame M.C.;
RL Submitted (JUL-2007) to UniProtKB.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=16512674; DOI=10.1021/pr050350q;
RA Reinders Y., Schulz I., Graef R., Sickmann A.;
RT "Identification of novel centrosomal proteins in Dictyostelium discoideum
RT by comparative proteomic approaches.";
RL J. Proteome Res. 5:589-598(2006).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=AX2;
RX PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA Soldati T.;
RT "Proteomics fingerprinting of phagosome maturation and evidence for the
RT role of a Galpha during uptake.";
RL Mol. Cell. Proteomics 5:2228-2243(2006).
CC -!- FUNCTION: Unknown, though MVP-alpha is required for normal vault
CC structure.
CC -!- SUBUNIT: The vault ribonucleoprotein particle is a huge (400 A x 670 A)
CC cage structure of 12.9 MDa. It consists of a dimer of half-vaults, with
CC each half-vault comprising 39 identical major vault protein (MVP)
CC chains. Dictyostelium is one of the few organisms in which the major
CC component is actually two proteins (alpha and beta).
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus {ECO:0000250}.
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DR EMBL; L08646; AAA03153.1; -; mRNA.
DR EMBL; AAFI02000005; EAL71928.1; -; Genomic_DNA.
DR PIR; A47132; A47132.
DR RefSeq; XP_646310.1; XM_641218.1.
DR AlphaFoldDB; P34118; -.
DR SMR; P34118; -.
DR IntAct; P34118; 1.
DR STRING; 44689.DDB0191259; -.
DR PaxDb; P34118; -.
DR EnsemblProtists; EAL71928; EAL71928; DDB_G0269156.
DR GeneID; 8617265; -.
DR KEGG; ddi:DDB_G0269156; -.
DR dictyBase; DDB_G0269156; mvpA.
DR eggNOG; ENOG502QPP0; Eukaryota.
DR HOGENOM; CLU_016171_0_0_1; -.
DR InParanoid; P34118; -.
DR OMA; VIRIKRY; -.
DR PhylomeDB; P34118; -.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR PRO; PR:P34118; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:dictyBase.
DR GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR GO; GO:1990904; C:ribonucleoprotein complex; TAS:dictyBase.
DR GO; GO:0009617; P:response to bacterium; HEP:dictyBase.
DR CDD; cd08825; MVP_shoulder; 1.
DR Gene3D; 2.30.30.550; -; 4.
DR Gene3D; 2.30.30.560; -; 2.
DR Gene3D; 3.30.479.30; -; 1.
DR InterPro; IPR036013; Band_7/SPFH_dom_sf.
DR InterPro; IPR039059; MVP.
DR InterPro; IPR041139; MVP_rep_dom.
DR InterPro; IPR043023; MVP_rep_sf.
DR InterPro; IPR021870; MVP_shoulder.
DR InterPro; IPR041134; Vault_2.
DR InterPro; IPR043179; Vault_2_sf.
DR InterPro; IPR040989; Vault_3.
DR InterPro; IPR041136; Vault_4.
DR InterPro; IPR002499; Vault_N.
DR PANTHER; PTHR14165; PTHR14165; 1.
DR Pfam; PF11978; MVP_shoulder; 1.
DR Pfam; PF01505; Vault; 4.
DR Pfam; PF17794; Vault_2; 2.
DR Pfam; PF17795; Vault_3; 1.
DR Pfam; PF17796; Vault_4; 1.
DR PROSITE; PS51224; MVP; 8.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Direct protein sequencing; Nucleus;
KW Reference proteome; Repeat; Ribonucleoprotein.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|Ref.3"
FT CHAIN 2..843
FT /note="Major vault protein alpha"
FT /id="PRO_0000158985"
FT REPEAT 2..56
FT /note="MVP 1"
FT REPEAT 57..111
FT /note="MVP 2"
FT REPEAT 112..163
FT /note="MVP 3"
FT REPEAT 164..216
FT /note="MVP 4"
FT REPEAT 217..272
FT /note="MVP 5"
FT REPEAT 273..324
FT /note="MVP 6"
FT REPEAT 325..376
FT /note="MVP 7"
FT REPEAT 377..442
FT /note="MVP 8"
FT REPEAT 443..505
FT /note="MVP 9"
FT REGION 643..663
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000269|Ref.3"
SQ SEQUENCE 843 AA; 94130 MW; 69F90EAC66CCB586 CRC64;
MADLNSVIRI KPFHFIHVLD NNTNVTRVEV GPQTFTRQDH EKLVSGPEPM IMIPQRNYCT
ISNPVVRNEN GSLVLDEYGQ VKLRHGDEEI RFSQEPFPLY PGEKISGKVT ALQVVAPLKA
LRLRALRDFT EAKVTHVAGD EWLFEGPATY LPRIDVRIEE EIKATIIGPN QALKLRANKA
CSDRSGVARK AGEEWLVRQH GAYLPGVDEK VVEIVNAYVL TDKKALHLKA TKTFLDETRK
TQRKAGEEWL VVSTDAETHI PDVYEQVVGE VHITTLSNRQ YCVVLDPIGA NGKPQLGHKQ
LRKGELAFFL NPGESLEGNK IHNIYVLTEQ EALLLRAKET FSIDGESHLA GDRWMIYGPC
DYVPPVQVEV VEKRESIPLD ENEGIYVRDI KTGKVASIKG QSYMLKANEE LWEKVLPRTV
EEVLAKESLN PEFTDNRDST RVVTYRAPHN SAVQIYDYKE KKSRVVFGPD LVMLGPDEHF
TVLSLSGDKP KRPHQIKAIA LFLGPDFMTD VVIVETSDHA RLSLKLSYNW EFKVDRSKIE
DAQKIFQVPD FVGDSCKAIA SRVRGAVAAV SFDDFHKRSA EVIRQSVFGL DESGEVRKNF
SFNSNNLVIT NIDIQSVEPV DQRTRDSLQK SVQLAIEITT KSQEAAARHE AERLEQGARG
RLERQKIHDE AQAELARKDL LQLQAQSAAV ESTGQATAEA QATAEAANIA AEANVKQAEL
KAQATKIRSE SEISLLKAKR ENELSYQKSI DELELTKQSD LAEIEASKFK AIVESIGRDT
LKSIACAGNE MQAKLLQGLG LKSFMITDGK SPLNLFDTAN GIIGNNNQMV NMSNAAKQSG
RRN
