MVPB_DICDI
ID MVPB_DICDI Reviewed; 846 AA.
AC P54659; Q54F22;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 25-MAY-2022, entry version 106.
DE RecName: Full=Major vault protein beta;
DE Short=MVP-beta;
GN Name=mvpB; ORFNames=DDB_G0291127;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=AX4;
RX PubMed=7622465; DOI=10.1074/jbc.270.28.16588;
RA Vasu S.K., Rome L.H.;
RT "Dictyostelium vaults: disruption of the major proteins reveals growth and
RT morphological defects and uncovers a new associated protein.";
RL J. Biol. Chem. 270:16588-16594(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP PROTEIN SEQUENCE OF 2-12; 31-40; 163-172; 199-207; 233-241; 316-327;
RP 389-400; 427-437; 463-475; 542-549; 578-590; 692-713; 795-804 AND 815-822,
RP CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, AND IDENTIFICATION
RP BY MASS SPECTROMETRY.
RA Bienvenut W.V., Patel H., Brunton V.G., Frame M.C.;
RL Submitted (JUL-2007) to UniProtKB.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=AX2;
RX PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA Soldati T.;
RT "Proteomics fingerprinting of phagosome maturation and evidence for the
RT role of a Galpha during uptake.";
RL Mol. Cell. Proteomics 5:2228-2243(2006).
CC -!- FUNCTION: Unknown, though MVP-beta is required for normal vault
CC structure.
CC -!- SUBUNIT: The vault ribonucleoprotein particle is a huge (400 A x 670 A)
CC cage structure of 12.9 MDa. It consists of a dimer of half-vaults, with
CC each half-vault comprising 39 identical major vault protein (MVP)
CC chains. Dictyostelium is one of the few organisms in which the major
CC component is actually two proteins (alpha and beta).
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus {ECO:0000250}.
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DR EMBL; Z37109; CAA85473.1; -; mRNA.
DR EMBL; AAFI02000175; EAL61847.1; -; Genomic_DNA.
DR PIR; A57241; A57241.
DR RefSeq; XP_635368.1; XM_630276.1.
DR AlphaFoldDB; P54659; -.
DR SMR; P54659; -.
DR STRING; 44689.DDB0191337; -.
DR PaxDb; P54659; -.
DR PRIDE; P54659; -.
DR EnsemblProtists; EAL61847; EAL61847; DDB_G0291127.
DR GeneID; 8628016; -.
DR KEGG; ddi:DDB_G0291127; -.
DR dictyBase; DDB_G0291127; mvpB.
DR eggNOG; ENOG502QPP0; Eukaryota.
DR HOGENOM; CLU_016171_0_0_1; -.
DR InParanoid; P54659; -.
DR OMA; AGEEWII; -.
DR PhylomeDB; P54659; -.
DR PRO; PR:P54659; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0031012; C:extracellular matrix; HDA:dictyBase.
DR GO; GO:0005634; C:nucleus; IDA:dictyBase.
DR GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR GO; GO:1990904; C:ribonucleoprotein complex; TAS:dictyBase.
DR CDD; cd08825; MVP_shoulder; 1.
DR Gene3D; 2.30.30.550; -; 4.
DR Gene3D; 2.30.30.560; -; 2.
DR Gene3D; 3.30.479.30; -; 1.
DR InterPro; IPR036013; Band_7/SPFH_dom_sf.
DR InterPro; IPR039059; MVP.
DR InterPro; IPR041139; MVP_rep_dom.
DR InterPro; IPR043023; MVP_rep_sf.
DR InterPro; IPR021870; MVP_shoulder.
DR InterPro; IPR041134; Vault_2.
DR InterPro; IPR043179; Vault_2_sf.
DR InterPro; IPR040989; Vault_3.
DR InterPro; IPR041136; Vault_4.
DR InterPro; IPR002499; Vault_N.
DR PANTHER; PTHR14165; PTHR14165; 1.
DR Pfam; PF11978; MVP_shoulder; 1.
DR Pfam; PF01505; Vault; 4.
DR Pfam; PF17794; Vault_2; 2.
DR Pfam; PF17795; Vault_3; 1.
DR Pfam; PF17796; Vault_4; 1.
DR PROSITE; PS51224; MVP; 8.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Direct protein sequencing; Nucleus;
KW Reference proteome; Repeat; Ribonucleoprotein.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|Ref.3"
FT CHAIN 2..846
FT /note="Major vault protein beta"
FT /id="PRO_0000158986"
FT REPEAT 2..60
FT /note="MVP 1"
FT REPEAT 61..115
FT /note="MVP 2"
FT REPEAT 116..172
FT /note="MVP 3"
FT REPEAT 173..225
FT /note="MVP 4"
FT REPEAT 226..280
FT /note="MVP 5"
FT REPEAT 281..332
FT /note="MVP 6"
FT REPEAT 333..388
FT /note="MVP 7"
FT REPEAT 389..458
FT /note="MVP 8"
FT REPEAT 459..521
FT /note="MVP 9"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000269|Ref.3"
SQ SEQUENCE 846 AA; 95073 MW; B4D5F610DE04DD2B CRC64;
MATPVQASTV IRVKPHHYIH VLDNNTNVTR VEVGPQTFTR QDHERVLNPT PLPMILIPPR
QYCIIENPII RDKEGKLVVD KFNQPKLRHG DQEIRFSQDP FPLYPGEVVI AQPVPLQVIQ
TNSALRLKCL RDFVEVKADK TTVSHIAGDE WLFEGPGTYY PRVEVQVVEP VRAVIIKENQ
ALKLRARLSF VDRRDVVRQV GEEWLVRESG AYLPGVSEEI VGFIQALVLT DRIAYQLLAT
RSFTDSFGKV RKAGEEWIIT QKDCDTYIPD VNEKVIRDIK AITLTNRQYC IVENPIDPKT
GKNKLGHKEL LQGDRTFFLM PGESLVKGIQ NVNVLSEDEA LLLSAKEEFV EKKGTTTIVH
KPGDLWMIYG PCDYIPPIQV DVVERRKRIP LDVNEGIYVR DIKTGKVRSV HGSSYMLLPN
EERWAKPLAP IVEELLKKSA SRDQNEEHNE SDRDSTKVIT YRVPHNAAVQ IFDYRKKEAR
VVFGPELVML EPDEEFTVLS LSGKIPKRPN HIRSLALFLG PDFMTDLITV ETADHARLSL
KLSYNWHFKV EQDNPSKLFA TSDFTGDLCK ATGSLVRAAV AASTFDNFHK HSSDIIQQAV
FGSTDGSSND CLYFETNGLV ITNIDVQSVE PVDQRTLDSL QKSVQLAIEI TTKSQEATAR
QEAERLEQMA RGELERQKII DEAKNEESRS KLVQLQAQSA AVESTGQAVA EARARAEAAI
IEAESEIKQA RLSTRAIEIE ALSEIENLKA KHIVEIQHIK SLNNLELIKA KESATIETTK
FENYVEALGS DTIKSIAQAP EETKAKLLAG LGLKSFMITD GKSPLNLFDT ANGLIADAQR
SEVDEE
