MVP_SLCV
ID MVP_SLCV Reviewed; 293 AA.
AC P21936;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1991, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Movement protein BC1;
DE AltName: Full=Movement protein BL1;
GN ORFNames=BC1, BL1;
OS Squash leaf curl virus (SLCV).
OC Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes;
OC Geplafuvirales; Geminiviridae; Begomovirus.
OX NCBI_TaxID=10829;
OH NCBI_TaxID=3662; Cucurbita moschata (Winter crookneck squash) (Cucurbita pepo var. moschata).
OH NCBI_TaxID=3663; Cucurbita pepo (Vegetable marrow) (Summer squash).
OH NCBI_TaxID=3885; Phaseolus vulgaris (Kidney bean) (French bean).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1984668; DOI=10.1016/0042-6822(91)90009-z;
RA Lazarowitz S.G., Lazdins I.B.;
RT "Infectivity and complete nucleotide sequence of the cloned genomic
RT components of a bipartite squash leaf curl geminivirus with a broad host
RT range phenotype.";
RL Virology 180:58-69(1991).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF PHE-35; ASN-67;
RP 78-ASP--ARG-80; LYS-79; 112-LYS-ASP-113; 120-TYR-TYR-121; 140-LYS--LYS-142;
RP 147-LYS-HIS-148; 208-TRP--LYS-211; 227-GLU-GLU-228 AND ASN-260.
RX PubMed=12242403; DOI=10.2307/3870094;
RA Sanderfoot A.A., Lazarowitz S.G.;
RT "Cooperation in viral movement: the geminivirus BL1 movement protein
RT interacts with BR1 and redirects it from the nucleus to the eell
RT periphery.";
RL Plant Cell 7:1185-1194(1995).
CC -!- FUNCTION: Transports viral genome to neighboring plant cells directly
CC through plasmosdesmata, without any budding. The movement protein
CC allows efficient cell to cell propagation, by bypassing the host cell
CC wall barrier. Begomovirus genome is shuttled out of nucleus by Nuclear
CC shuttle protein (NSP) and the movement protein transports the DNA-NSP
CC complex to cell plasmodesmata and facilitates further movement across
CC the cell wall. {ECO:0000269|PubMed:12242403}.
CC -!- SUBUNIT: Binds to dimeric supercoiled plasmid DNA. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Host cell membrane {ECO:0000269|PubMed:12242403};
CC Peripheral membrane protein {ECO:0000269|PubMed:12242403}; Cytoplasmic
CC side {ECO:0000269|PubMed:12242403}. Host microsome membrane
CC {ECO:0000269|PubMed:12242403}; Peripheral membrane protein
CC {ECO:0000269|PubMed:12242403}; Cytoplasmic side
CC {ECO:0000269|PubMed:12242403}. Host endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:12242403}; Peripheral membrane protein
CC {ECO:0000269|PubMed:12242403}; Cytoplasmic side
CC {ECO:0000269|PubMed:12242403}. Note=Found on ER-derived vesicles.
CC -!- PTM: Phosphorylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the begomovirus movement protein BC1 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC32409.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M38182; AAC32409.1; ALT_INIT; Genomic_DNA.
DR PIR; B36785; QQCVBV.
DR RefSeq; NP_047248.1; NC_001937.1.
DR GeneID; 956399; -.
DR KEGG; vg:956399; -.
DR Proteomes; UP000009151; Genome.
DR GO; GO:0044167; C:host cell endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR InterPro; IPR000211; Gemini_BL.
DR Pfam; PF00845; Gemini_BL1; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Host cell membrane; Host endoplasmic reticulum; Host membrane;
KW Host microsome; Membrane; Phosphoprotein; Reference proteome; Transport;
KW Viral movement protein.
FT CHAIN 1..293
FT /note="Movement protein BC1"
FT /id="PRO_0000222257"
FT MUTAGEN 35
FT /note="F->A: No effect on subcellular location. Reduced
FT levels of infectivity and very attenuated disease symptoms.
FT No effect on NSP relocalization."
FT /evidence="ECO:0000269|PubMed:12242403"
FT MUTAGEN 67
FT /note="N->A: No effect on subcellular location. Reduced
FT levels of infectivity and very attenuated disease symptoms.
FT No effect on NSP relocalization."
FT /evidence="ECO:0000269|PubMed:12242403"
FT MUTAGEN 78..80
FT /note="DKR->AKA: Mislocalizes to the cytoplasm. Complete
FT loss of infectivity."
FT /evidence="ECO:0000269|PubMed:12242403"
FT MUTAGEN 79
FT /note="K->A: No effect on subcellular location. No effect
FT on infectivity. No effect on NSP relocalization."
FT /evidence="ECO:0000269|PubMed:12242403"
FT MUTAGEN 112..113
FT /note="KD->AA: Mislocalizes to the cytoplasm. Complete loss
FT of infectivity."
FT /evidence="ECO:0000269|PubMed:12242403"
FT MUTAGEN 120..121
FT /note="YY->AA: No effect on subcellular location. Complete
FT loss of infectivity."
FT /evidence="ECO:0000269|PubMed:12242403"
FT MUTAGEN 140..142
FT /note="KLK->ALA: No effect on subcellular location. Loss of
FT NSP relocalization. Reduced levels of infectivity and very
FT attenuated disease symptoms."
FT /evidence="ECO:0000269|PubMed:12242403"
FT MUTAGEN 147..148
FT /note="KH->AA: No effect on subcellular location. Loss of
FT NSP relocalization. Reduced levels of infectivity and very
FT attenuated disease symptoms."
FT /evidence="ECO:0000269|PubMed:12242403"
FT MUTAGEN 208..211
FT /note="WATK->AATA: Mislocalizes to the cytoplasm. Complete
FT loss of infectivity."
FT /evidence="ECO:0000269|PubMed:12242403"
FT MUTAGEN 227..228
FT /note="EE->AA: No effect on subcellular location. No effect
FT on infectivity."
FT /evidence="ECO:0000269|PubMed:12242403"
FT MUTAGEN 260
FT /note="N->A: Mislocalizes to the cytoplasm. Almost complete
FT loss of infectivity."
FT /evidence="ECO:0000269|PubMed:12242403"
SQ SEQUENCE 293 AA; 33179 MW; 25C28BBC242DC5A5 CRC64;
MGSQLVPPPS AFNYIESQRD EFQLSHDLTE IVLQFPSTAS QITARLSRSC MKIDHCVIEY
RQQVPINASG TVIVEIHDKR MTDNESLQAS WTFPIRCNID LHYFSSSFFS LKDPIPWKLY
YRVSDSNVHQ MTHFAKFKGK LKLSSAKHSV DIPFRAPTVK ILAKQFSEKD IDFWHVGYGK
WERRLVKSAS SSRFGLRGPI EINPGESWAT KSAIGPTNRN ADLDIEEELL PYRELNRLGT
NILDPGESAS IVGIQRSQSN ITMSMSQLNE LVRSTVHECI KTSCIPSTPK SLS
