ID   MVP_TBSVC               Reviewed;         189 AA.
AC   P11691;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1989, sequence version 1.
DT   02-DEC-2020, entry version 65.
DE   RecName: Full=Movement protein;
DE   AltName: Full=p22;
OS   Tomato bushy stunt virus (strain Cherry) (TBSV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC   Tolivirales; Tombusviridae; Procedovirinae; Tombusvirus.
OX   NCBI_TaxID=12147;
OH   NCBI_TaxID=4072; Capsicum annuum (Capsicum pepper).
OH   NCBI_TaxID=3749; Malus.
OH   NCBI_TaxID=3766; Pyrus (pears).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH   NCBI_TaxID=4111; Solanum melongena (Eggplant) (Aubergine).
OH   NCBI_TaxID=29777; Tolmiea menziesii.
OH   NCBI_TaxID=13305; Tulipa.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2922927; DOI=10.1016/0042-6822(89)90039-1;
RA   Hillman B.I., Hearne P.Q., Rochon D., Morris T.J.;
RT   "Organization of tomato bushy stunt virus genome: characterization of the
RT   coat protein gene and the 3' terminus.";
RL   Virology 169:42-50(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2353450; DOI=10.1016/0042-6822(90)90468-7;
RA   Hearne P.Q., Knorr D.A., Hillman B.I., Morris T.J.;
RT   "The complete genome structure and synthesis of infectious RNA from clones
RT   of tomato bushy stunt virus.";
RL   Virology 177:141-151(1990).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, RNA-BINDING, PHOSPHORYLATION, MUTAGENESIS
RP   OF 2-ASP--GLU-6; 32-GLU--GLU-35 AND GLU-103, AND INTERACTION WITH NICOTIANA
RP   BENTHAMIANA PROTEIN HFI22.
RX   PubMed=12177465; DOI=10.1104/pp.004754;
RA   Desvoyes B., Faure-Rabasse S., Chen M.H., Park J.W., Scholthof H.B.;
RT   "A novel plant homeodomain protein interacts in a functionally relevant
RT   manner with a virus movement protein.";
RL   Plant Physiol. 129:1521-1532(2002).
CC   -!- FUNCTION: Transports viral genome to neighboring plant cells directly
CC       through plasmosdesmata, without any budding. The movement protein
CC       allows efficient cell to cell propagation, by bypassing the host cell
CC       wall barrier. Displays RNA-binding activity.
CC       {ECO:0000269|PubMed:12177465}.
CC   -!- SUBUNIT: Interacts with host protein HFI22.
CC       {ECO:0000269|PubMed:12177465}.
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000269|PubMed:12177465}.
CC   -!- PTM: Phosphorylated. {ECO:0000269|PubMed:12177465}.
CC   -!- SIMILARITY: Belongs to the tombusvirus/aureusvirus movement protein p22
CC       family. {ECO:0000305}.
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DR   EMBL; M21958; AAB02537.1; -; Genomic_RNA.
DR   PIR; JA0115; NKVGTB.
DR   RefSeq; NP_062900.1; NC_001554.1.
DR   GeneID; 1493954; -.
DR   KEGG; vg:1493954; -.
DR   Proteomes; UP000001666; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019028; C:viral capsid; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   InterPro; IPR005332; TBSV_p22.
DR   Pfam; PF03558; TBSV_P22; 1.
PE   1: Evidence at protein level;
KW   Host membrane; Host-virus interaction; Membrane; Phosphoprotein;
KW   Reference proteome; RNA-binding; Transport; Viral movement protein.
FT   CHAIN           1..189
FT                   /note="Movement protein"
FT                   /id="PRO_0000222891"
FT   MUTAGEN         2..6
FT                   /note="DTEYE->ATAYA: Movement defective. No effect on
FT                   interaction with host protein HFI22. No effect on
FT                   association with host membrane."
FT                   /evidence="ECO:0000269|PubMed:12177465"
FT   MUTAGEN         32..35
FT                   /note="EDQE->AAQA: Movement defective. Loss of interaction
FT                   with host protein HFI22."
FT                   /evidence="ECO:0000269|PubMed:12177465"
FT   MUTAGEN         103
FT                   /note="E->A: Movement defective. Loss of interaction with
FT                   host protein HFI22. No effect on association with host
FT                   membrane."
FT                   /evidence="ECO:0000269|PubMed:12177465"
SQ   SEQUENCE   189 AA;  21608 MW;  B9DE1C79465652F9 CRC64;
     MDTEYEQVNK PWNELYKETT LGNKLTVNVG MEDQEVPLLP SNFLTKVRVG LSGGYITMRR
     IRIKIIPLVS RKAGVSGKLY LRDISDTTGR KLHCTESLDL GREIRLTMQH LDFSVSTRSD
     VPIVFGFEEL VSPFLEGREL FSISVRWQFG LSKNCYSLPQ SKWKVMYQED ALKVLRPSKK
     KASKTDSSV