MVP_TOML
ID MVP_TOML Reviewed; 264 AA.
AC P69513; P03584;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 29-SEP-2021, entry version 45.
DE RecName: Full=Movement protein;
DE AltName: Full=30 kDa protein;
DE AltName: Full=Cell-to-cell transport protein;
GN Name=MP;
OS Tomato mosaic virus (strain L) (ToMV) (TMV strain tomato).
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Martellivirales; Virgaviridae; Tobamovirus.
OX NCBI_TaxID=12252;
OH NCBI_TaxID=4151; Antirrhinum majus (Garden snapdragon).
OH NCBI_TaxID=4071; Capsicum (peppers).
OH NCBI_TaxID=46246; Delphinium.
OH NCBI_TaxID=4101; Petunia.
OH NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH NCBI_TaxID=13707; Tagetes (marigolds).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=6304642; DOI=10.1093/nar/11.11.3767;
RA Takamatsu N., Ohno T., Meshi T., Okada Y.;
RT "Molecular cloning and nucleotide sequence of the 30K and the coat protein
RT cistron of TMV (tomato strain) genome.";
RL Nucleic Acids Res. 11:3767-3778(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=6549393; DOI=10.1093/oxfordjournals.jbchem.a135026;
RA Ohno T., Aoyagi M., Yamanashi Y., Saito H., Ikawa S., Meshi T., Okada Y.;
RT "Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and
RT comparison with the common strain genome.";
RL J. Biochem. 96:1915-1923(1984).
RN [3]
RP VARIANTS PHE-68 AND LYS-133.
RX PubMed=2535549; DOI=10.2307/3868972;
RA Meshi T., Motoyoshi F., Maeda T., Yoshiwoka S., Watanabe H., Okada Y.;
RT "Mutations in the tobacco mosaic virus 30-kD protein gene overcome Tm-2
RT resistance in tomato.";
RL Plant Cell 1:515-522(1989).
RN [4]
RP VARIANTS GLU-130; GLU-184; ARG-238 AND GLU-244.
RX PubMed=8411345; DOI=10.1128/jvi.67.11.6432-6438.1993;
RA Weber H., Schultze S., Pfitzner A.J.P.;
RT "Two amino acid substitutions in the tomato mosaic virus 30-kilodalton
RT movement protein confer the ability to overcome the Tm-2(2) resistance gene
RT in the tomato.";
RL J. Virol. 67:6432-6438(1993).
RN [5]
RP MUTAGENESIS OF 235-LYS--TYR-264, AND VARIANTS GLU-130; GLU-184; ARG-238 AND
RP GLU-244.
RX PubMed=9612948; DOI=10.1094/mpmi.1998.11.6.498;
RA Weber H., Pfitzner A.J.P.;
RT "Tm-2(2) resistance in tomato requires recognition of the carboxy terminus
RT of the movement protein of tomato mosaic virus.";
RL Mol. Plant Microbe Interact. 11:498-503(1998).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 235-LYS--TYR-264, AND
RP VARIANTS PHE-68; GLU-130; LYS-133; GLU-184; ARG-238 AND GLU-244.
RX PubMed=15290376; DOI=10.1007/s00705-004-0312-0;
RA Weber H., Ohnesorge S., Silber M.V., Pfitzner A.J.P.;
RT "The Tomato mosaic virus 30 kDa movement protein interacts differentially
RT with the resistance genes Tm-2 and Tm-2(2).";
RL Arch. Virol. 149:1499-1514(2004).
RN [7]
RP INTERACTION WITH HOST NICOTIANA BENTHAMIANA RBCS, AND SUBCELLULAR LOCATION.
RX PubMed=23148080; DOI=10.1104/pp.112.209213;
RA Zhao J., Liu Q., Zhang H., Jia Q., Hong Y., Liu Y.;
RT "The rubisco small subunit is involved in tobamovirus movement and Tm-2(2)-
RT mediated extreme resistance.";
RL Plant Physiol. 161:374-383(2013).
CC -!- FUNCTION: Transports viral genome to neighboring plant cells directly
CC through plasmosdesmata, without any budding. The movement protein
CC allows efficient cell to cell propagation, by bypassing the host cell
CC wall barrier. Forms a ribonucleoprotein complex with viral RNA (By
CC similarity). Triggers host hypersensitive defense reaction in
CC incompatible tomato plants harboring Tm-2, Tm-2nv or Tm-2(2) ToMV
CC resistance (R) proteins (PubMed:15290376).
CC {ECO:0000250|UniProtKB:P03583, ECO:0000269|PubMed:15290376}.
CC -!- FUNCTION: Transports viral genome to neighboring plant cells directly
CC through plasmosdesmata, without any budding. The movement protein
CC allows efficient cell to cell propagation, by bypassing the host cell
CC wall barrier. Forms a ribonucleoprotein complex with viral RNA. Binds
CC microtubules and modulates microtubule stability. Can bind double-
CC stranded DNA. Triggers host hypersensitive defense reaction in
CC incompatible plants harboring resistance (R) proteins.
CC {ECO:0000250|UniProtKB:P03583}.
CC -!- SUBUNIT: May bind to host RBCS at the plasmodesmata (e.g. Nicotiana
CC benthamiana RBCS AC A0A0S4IJL0); this interaction seems required for
CC viral systemic movement (PubMed:23148080). In resistant plants,
CC interacts with host MBP2C at host microtubules; this interaction
CC prevents virus cell to cell movement. In resistant plants, interacts
CC with host resistance (R) protein (e.g. tomato ToMV resistance protein
CC TM-2(2), AC Q71BG9) at the host plasma membrane; this interaction
CC triggers host defense responses leading to programmed cell death (By
CC similarity). {ECO:0000250|UniProtKB:A0A0S4IJL0,
CC ECO:0000250|UniProtKB:P03583, ECO:0000269|PubMed:23148080}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm, host cytoskeleton
CC {ECO:0000305|PubMed:15290376}. Host cell junction, host plasmodesma
CC {ECO:0000269|PubMed:23148080, ECO:0000305|PubMed:15290376}. Note=Binds
CC to the host cytoskeleton before being transported to the host
CC plasmodesmata (Probable). Observed in virus replication complexes
CC (VRCs) of tobamovirus infected host cells (PubMed:23148080). In
CC resistant plants, targeted to the host plasma membrane via the
CC interaction with host resistance (R) protein TM-2.
CC {ECO:0000250|UniProtKB:P03583, ECO:0000269|PubMed:23148080,
CC ECO:0000305|PubMed:15290376}.
CC -!- SIMILARITY: Belongs to the tobamovirus movement protein family.
CC {ECO:0000305}.
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DR EMBL; X02144; CAA26083.1; -; Genomic_RNA.
DR Proteomes; UP000001451; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0044219; C:host cell plasmodesma; IDA:UniProtKB.
DR GO; GO:0044163; C:host cytoskeleton; NAS:UniProtKB.
DR GO; GO:0019034; C:viral replication complex; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0034053; P:modulation by symbiont of host defense-related programmed cell death; IEA:UniProtKB-KW.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR InterPro; IPR001022; TMV_movement.
DR InterPro; IPR028919; Viral_movement.
DR Pfam; PF01107; MP; 1.
DR PRINTS; PR00964; MOVEMENT.
PE 1: Evidence at protein level;
KW Host cell junction; Host cytoplasm; Host cytoskeleton;
KW Hypersensitive response elicitation; Reference proteome; RNA-binding;
KW Transport; Viral movement protein.
FT CHAIN 1..264
FT /note="Movement protein"
FT /id="PRO_0000144970"
FT REGION 211..264
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 245..264
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 68
FT /note="C -> F (in strain: Ltbl; ability to grow in tomato
FT plants harboring the ToMV resistance protein Tm-2)"
FT /evidence="ECO:0000269|PubMed:15290376,
FT ECO:0000269|PubMed:2535549"
FT VARIANT 130
FT /note="K -> E (in strain: ToMV2(2); ability to grow in
FT tomato plants harboring the ToMV resistance protein Tm-
FT 2(2))"
FT /evidence="ECO:0000269|PubMed:15290376,
FT ECO:0000269|PubMed:8411345"
FT VARIANT 133
FT /note="E -> K (in strain: Ltbl; ability to grow in tomato
FT plants harboring the ToMV resistance protein Tm-2)"
FT /evidence="ECO:0000269|PubMed:15290376,
FT ECO:0000269|PubMed:2535549"
FT VARIANT 184
FT /note="G -> E (in strain: ToMV2(2); ability to grow in
FT tomato plants harboring the ToMV resistance protein Tm-
FT 2(2))"
FT /evidence="ECO:0000269|PubMed:15290376,
FT ECO:0000269|PubMed:8411345"
FT VARIANT 238
FT /note="S -> R (in strain: ToMV2(2); ability to grow in
FT tomato plants harboring the ToMV resistance protein Tm-
FT 2(2))"
FT /evidence="ECO:0000269|PubMed:15290376,
FT ECO:0000269|PubMed:8411345"
FT VARIANT 244
FT /note="K -> E (in strain: ToMV2(2); ability to grow in
FT tomato plants harboring the ToMV resistance protein Tm-
FT 2(2))"
FT /evidence="ECO:0000269|PubMed:15290376,
FT ECO:0000269|PubMed:8411345"
FT MUTAGEN 235..264
FT /note="Missing: Confers the ability to grow in tomato
FT plants harboring the ToMV resistance protein Tm-2(2)."
FT /evidence="ECO:0000269|PubMed:15290376,
FT ECO:0000269|PubMed:9612948"
SQ SEQUENCE 264 AA; 29291 MW; 3B01EB2359AF9C4E CRC64;
MALVVKGKVN INEFIDLSKS EKLLPSMFTP VKSVMVSKVD KIMVHENESL SEVNLLKGVK
LIEGGYVCLV GLVVSGEWNL PDNCRGGVSV CMVDKRMERA DEATLGSYYT AAAKKRFQFK
VVPNYGITTK DAEKNIWQVL VNIKNVKMSA GYCPLSLEFV SVCIVYKNNI KLGLREKVTS
VNDGGPMELS EEVVDEFMEN VPMSVRLAKF RTKSSKRGPK NNNNLGKGRS GGRPKPKSFD
EVEKEFDNLI EDEAETSVAD SDSY
