MVP_TSWV1
ID MVP_TSWV1 Reviewed; 301 AA.
AC P36292;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 25-MAY-2022, entry version 63.
DE RecName: Full=Movement protein;
DE Short=MVP;
DE AltName: Full=Non-structural movement protein;
DE Short=NSm;
GN Name=NSM;
OS Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Ellioviricetes; Bunyavirales; Tospoviridae; Orthotospovirus.
OX NCBI_TaxID=36413;
OH NCBI_TaxID=133901; Frankliniella occidentalis (Western flower thrips) (Euthrips occidentalis).
OH NCBI_TaxID=163899; Scirtothrips dorsalis (Chilli thrips).
OH NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH NCBI_TaxID=161014; Thrips tabaci.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=1431808; DOI=10.1099/0022-1317-73-11-2795;
RA Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R.;
RT "The nucleotide sequence of the M RNA segment of tomato spotted wilt virus,
RT a bunyavirus with two ambisense RNA segments.";
RL J. Gen. Virol. 73:2795-2804(1992).
RN [2]
RP ERRATUM OF PUBMED:1431808.
RX PubMed=8468562; DOI=10.1099/0022-1317-74-4-790;
RA Kormelink R., de Haan P., Meurs C., Peters D., Goldbach R.;
RL J. Gen. Virol. 74:790-790(1993).
RN [3]
RP CHARACTERIZATION, AND SUBCELLULAR LOCATION.
RX PubMed=8128638; DOI=10.1006/viro.1994.1162;
RA Kormelink R., Storms M., Van Lent J., Peters D., Goldbach R.;
RT "Expression and subcellular location of the NSM protein of tomato spotted
RT wilt virus (TSWV), a putative viral movement protein.";
RL Virology 200:56-65(1994).
RN [4]
RP FUNCTION.
RX PubMed=8553550; DOI=10.1006/viro.1995.0059;
RA Storms M.M., Kormelink R., Peters D., Van Lent J.W., Goldbach R.W.;
RT "The nonstructural NSm protein of tomato spotted wilt virus induces tubular
RT structures in plant and insect cells.";
RL Virology 214:485-493(1995).
RN [5]
RP FUNCTION.
RX PubMed=10640565; DOI=10.1099/0022-1317-81-1-257;
RA Melcher U.;
RT "The '30K' superfamily of viral movement proteins.";
RL J. Gen. Virol. 81:257-266(2000).
RN [6]
RP FUNCTION.
RX PubMed=16112159; DOI=10.1016/j.virol.2005.06.050;
RA Lewandowski D.J., Adkins S.;
RT "The tubule-forming NSm protein from Tomato spotted wilt virus complements
RT cell-to-cell and long-distance movement of Tobacco mosaic virus hybrids.";
RL Virology 342:26-37(2005).
RN [7]
RP INTERACTION WITH HOST ARABIDOPSIS THALIANA AT4G26020 PROTEIN.
RX PubMed=16903353; DOI=10.1094/mpmi-19-0874;
RA Paape M., Solovyev A.G., Erokhina T.N., Minina E.A., Schepetilnikov M.V.,
RA Lesemann D.-E., Schiemann J., Morozov S.Y., Kellmann J.-W.;
RT "At-4/1, an interactor of the Tomato spotted wilt virus movement protein,
RT belongs to a new family of plant proteins capable of directed intra- and
RT intercellular trafficking.";
RL Mol. Plant Microbe Interact. 19:874-883(2006).
RN [8]
RP FUNCTION.
RX PubMed=19481775; DOI=10.1016/j.virol.2009.04.027;
RA Li W., Lewandowski D.J., Hilf M.E., Adkins S.;
RT "Identification of domains of the Tomato spotted wilt virus NSm protein
RT involved in tubule formation, movement and symptomatology.";
RL Virology 390:110-121(2009).
CC -!- FUNCTION: Viral movemement protein which is responsible for cell-to
CC cell spread of viral genome. Increases the size exclusion limit (SEL)
CC of plasmodesmata by forming tubules structures, thereby allowing viral
CC ribonucleocapsids to spread directly to neighboring cells.
CC {ECO:0000269|PubMed:10640565, ECO:0000269|PubMed:16112159,
CC ECO:0000269|PubMed:19481775, ECO:0000269|PubMed:8553550}.
CC -!- SUBUNIT: Oligomerizes to form tubule structures through host
CC plasmodesma. Interacts with host A.thaliana At4g26020 protein, which is
CC involved in intra- and inter-cellular trafficking.
CC {ECO:0000269|PubMed:16903353}.
CC -!- SUBCELLULAR LOCATION: Host cell junction, host plasmodesma
CC {ECO:0000269|PubMed:8128638}. Note=Colocalizes with viral nucleocapsid
CC aggregates in host cytoplasm.
CC -!- SIMILARITY: Belongs to the tospovirus movement protein family.
CC {ECO:0000305}.
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DR EMBL; S48091; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR PIR; JQ1927; JQ1927.
DR Proteomes; UP000006674; Genome.
DR GO; GO:0044219; C:host cell plasmodesma; IDA:UniProtKB.
DR GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR InterPro; IPR006889; Bunya_NSM.
DR InterPro; IPR000603; MPV.
DR Pfam; PF00803; 3A; 1.
DR PIRSF; PIRSF003959; NS-M_TospoV; 1.
PE 1: Evidence at protein level;
KW Host cell junction; Host-virus interaction; Reference proteome; Transport;
KW Viral movement protein.
FT CHAIN 1..301
FT /note="Movement protein"
FT /id="PRO_0000221987"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 19..159
FT /note="Essential for tubule formation and cell-to-cell
FT movement"
FT REGION 209..283
FT /note="Essential for tubule formation and cell-to-cell
FT movement"
FT REGION 282..301
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 284..288
FT /note="Essential for long-distance movement"
SQ SEQUENCE 301 AA; 33564 MW; B00E16BEE6BA84C6 CRC64;
MLTLFGNKRP SKSAGKDEGP LVSLAKHNGS VEVSKPWSSS DEKLALTKAM DASKGKILLN
IEGTSSFGTY ESDSIIESEG YDLSARMIVD TNHHISNWKN DLFVGNGKQN ANKVIKICPT
WDSRKQYMMI SRIVIWVCPT IPNPTGKLVV ALIDPNMPSG KQVILKGQGT ITDPICFVFY
LNWSIPKMNN TPENCCQLHL MCSQEYKKGV SFGSVMYSWT KEFGDSPRAD KDKCMVIPLN
RAIRARSQAF IEACKLIIPK CNSEKQIKKQ LKELSSNLER SVEEEEEGIS DSVAQLSFDE
I
