MWL1_ARATH
ID MWL1_ARATH Reviewed; 175 AA.
AC A2RVU1; A0A1P8AQR9; Q9C6W2;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 08-MAY-2019, sequence version 2.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Protein MODIFYING WALL LIGNIN-1 {ECO:0000303|PubMed:26930070};
DE Short=MWL-1 {ECO:0000303|PubMed:26930070};
DE Flags: Precursor;
GN Name=MWL1 {ECO:0000303|PubMed:26930070};
GN OrderedLocusNames=At1g31720 {ECO:0000312|Araport:AT1G31720};
GN ORFNames=F27M3.8 {ECO:0000312|EMBL:AAG60140.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RA Kim C.J., Bautista V.R., Chen H., De Los Reyes C., Wu S.Y., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND INTERACTION WITH
RP CRK19.
RC STRAIN=cv. Columbia;
RX PubMed=26930070; DOI=10.1371/journal.pone.0150254;
RA Mewalal R., Mizrachi E., Coetzee B., Mansfield S.D., Myburg A.A.;
RT "The Arabidopsis domain of unknown function 1218 (DUF1218) containing
RT proteins, MODIFYING WALL LIGNIN-1 and 2 (At1g31720/MWL-1 and At4g19370/MWL-
RT 2) function redundantly to alter secondary cell wall lignin content.";
RL PLoS ONE 11:E0150254-E0150254(2016).
CC -!- FUNCTION: Together with MWL2, contributes to secondary cell wall
CC biology, specifically lignin biosynthesis.
CC {ECO:0000269|PubMed:26930070}.
CC -!- SUBUNIT: Interacts with CRK19. {ECO:0000269|PubMed:26930070}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:26930070};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=A2RVU1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A2RVU1-2; Sequence=VSP_060122;
CC Name=3;
CC IsoId=A2RVU1-3; Sequence=VSP_060121;
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Double mutants lacking both
CC MWL1 and MWL2 exhibit smaller rosettes with a decrease in rosette fresh
CC weight and stem height associated with a reduction in total lignin
CC content and an increase in syringyl/guaiacyl (S/G) monomer ratio.
CC {ECO:0000269|PubMed:26930070}.
CC -!- SIMILARITY: Belongs to the DESIGUAL family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG60140.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC074360; AAG60140.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31385.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM59003.1; -; Genomic_DNA.
DR EMBL; BT030082; ABN04820.1; -; mRNA.
DR RefSeq; NP_001321400.1; NM_001332972.1.
DR RefSeq; NP_174453.2; NM_102907.2.
DR AlphaFoldDB; A2RVU1; -.
DR IntAct; A2RVU1; 1.
DR STRING; 3702.AT1G31720.1; -.
DR PaxDb; A2RVU1; -.
DR EnsemblPlants; AT1G31720.1; AT1G31720.1; AT1G31720.
DR GeneID; 840059; -.
DR Gramene; AT1G31720.1; AT1G31720.1; AT1G31720.
DR KEGG; ath:AT1G31720; -.
DR Araport; AT1G31720; -.
DR TAIR; locus:2028596; AT1G31720.
DR eggNOG; ENOG502S1EJ; Eukaryota.
DR HOGENOM; CLU_110866_0_0_1; -.
DR InParanoid; A2RVU1; -.
DR OrthoDB; 1145302at2759; -.
DR PhylomeDB; A2RVU1; -.
DR PRO; PR:A2RVU1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; A2RVU1; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0009809; P:lignin biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009808; P:lignin metabolic process; IGI:TAIR.
DR InterPro; IPR009606; DEAL/Modifying_wall_lignin1/2.
DR Pfam; PF06749; DUF1218; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Lignin biosynthesis; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..175
FT /note="Protein MODIFYING WALL LIGNIN-1"
FT /id="PRO_0000446979"
FT TOPO_DOM 25..52
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 74..86
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..135
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 157..175
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT VAR_SEQ 1
FT /note="M -> MEIQKQDNRDGRPKSFLFF (in isoform 3)"
FT /id="VSP_060121"
FT VAR_SEQ 23..32
FT /note="Missing (in isoform 2)"
FT /id="VSP_060122"
SQ SEQUENCE 175 AA; 19317 MW; 74308F8FA436F721 CRC64;
MFIFLFGLAA FFLCLSAEFQ KAKALLRAQV FLKGKDLKWD GESCYLPENR AFGLGIAALV
CVSVAQIVGN VVICRGFTKT DKTRTTIFCI ILLLFSWVNF AVAVTLISVG ASMNREQIYG
KGWLNRECYL VKDGVFAASG FLSVTTMAAI LGAFAFKVKP SLQVENHDKR HTQNV
