MWL2_ARATH
ID MWL2_ARATH Reviewed; 217 AA.
AC O65708;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Protein MODIFYING WALL LIGNIN-2 {ECO:0000303|PubMed:26930070};
DE Short=MWL-2 {ECO:0000303|PubMed:26930070};
DE Flags: Precursor;
GN Name=MWL2 {ECO:0000303|PubMed:26930070};
GN OrderedLocusNames=At4g19370 {ECO:0000312|Araport:AT4G19370};
GN ORFNames=T5K18.150 {ECO:0000312|EMBL:AEE84173.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=16244158; DOI=10.1104/pp.105.063479;
RA Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L.,
RA Ayele M., Haas B.J., Wu H.C., Town C.D.;
RT "Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2
RT reveals numerous transcript variants.";
RL Plant Physiol. 139:1323-1337(2005).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=26930070; DOI=10.1371/journal.pone.0150254;
RA Mewalal R., Mizrachi E., Coetzee B., Mansfield S.D., Myburg A.A.;
RT "The Arabidopsis domain of unknown function 1218 (DUF1218) containing
RT proteins, MODIFYING WALL LIGNIN-1 and 2 (At1g31720/MWL-1 and At4g19370/MWL-
RT 2) function redundantly to alter secondary cell wall lignin content.";
RL PLoS ONE 11:E0150254-E0150254(2016).
CC -!- FUNCTION: Together with MWL1, contributes to secondary cell wall
CC biology, specifically lignin biosynthesis.
CC {ECO:0000269|PubMed:26930070}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:26930070};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Double mutants lacking both
CC MWL1 and MWL2 exhibit smaller rosettes with a decrease in rosette fresh
CC weight and stem height associated with a reduction in total lignin
CC content and an increase in syringyl/guaiacyl (S/G) monomer ratio.
CC {ECO:0000269|PubMed:26930070}.
CC -!- SIMILARITY: Belongs to the DESIGUAL family. {ECO:0000305}.
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DR EMBL; AL022580; CAA18624.1; -; Genomic_DNA.
DR EMBL; AL161550; CAB78939.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE84173.1; -; Genomic_DNA.
DR EMBL; AY954860; AAX55186.1; -; mRNA.
DR EMBL; DQ132708; AAZ52738.1; -; mRNA.
DR PIR; T05820; T05820.
DR RefSeq; NP_193672.1; NM_118057.3.
DR AlphaFoldDB; O65708; -.
DR SMR; O65708; -.
DR STRING; 3702.AT4G19370.1; -.
DR PaxDb; O65708; -.
DR PRIDE; O65708; -.
DR EnsemblPlants; AT4G19370.1; AT4G19370.1; AT4G19370.
DR GeneID; 827678; -.
DR Gramene; AT4G19370.1; AT4G19370.1; AT4G19370.
DR KEGG; ath:AT4G19370; -.
DR Araport; AT4G19370; -.
DR TAIR; locus:2140391; AT4G19370.
DR eggNOG; ENOG502S1EJ; Eukaryota.
DR HOGENOM; CLU_110866_0_0_1; -.
DR InParanoid; O65708; -.
DR OMA; RYEEGGC; -.
DR OrthoDB; 1145302at2759; -.
DR PhylomeDB; O65708; -.
DR PRO; PR:O65708; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O65708; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0009809; P:lignin biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009808; P:lignin metabolic process; IGI:TAIR.
DR InterPro; IPR009606; DEAL/Modifying_wall_lignin1/2.
DR Pfam; PF06749; DUF1218; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Lignin biosynthesis; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..217
FT /note="Protein MODIFYING WALL LIGNIN-2"
FT /id="PRO_0000446980"
FT TOPO_DOM 24..51
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 73..94
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 116..137
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..217
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT CARBOHYD 197
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 213
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 217 AA; 24387 MW; D5DB695407191211 CRC64;
MHNLFLYSVV FSLGLVSFIT CFAAEFKRTQ KEDIRWDTER NCYVPGSHAF GLGSAAVLCF
CLAQIVGNIV VFRNHRTRTK REDGYKITDL TLPTVLLLLS WSNFVVVVLI LSTAISMSRA
QAYGEGWLDE DCYLVKDGVF AASGCLAILG LGALTISATR IKVKKQQQLV QVVIKDQNQD
QRRSMEEEQK HDEHQTNKSE SVIHLVEEVS STNISRI
