A14_VACCC
ID A14_VACCC Reviewed; 90 AA.
AC P20991;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 23-FEB-2022, entry version 74.
DE RecName: Full=Virion membrane protein A14;
GN ORFNames=A14L;
OS Vaccinia virus (strain Copenhagen) (VACV).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10249;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA Paoletti E.;
RT "The complete DNA sequence of vaccinia virus.";
RL Virology 179:247-266(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA Paoletti E.;
RT "Appendix to 'The complete DNA sequence of vaccinia virus'.";
RL Virology 179:517-563(1990).
CC -!- FUNCTION: Envelope protein which is a major component of the mature
CC virion (MV) membrane. Essential for membrane biogenesis. Is required,
CC together with A17, to form bona fide crescents, which can progress to
CC form the immature virion (IV) membrane. A14 and A17 form a lattice that
CC is stabilized by disulfide bonds and serves as an anchor within the
CC viral membrane to which several other proteins important in virion
CC structure and morphogenesis attach (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. Interacts with A17.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}. Note=Component of the mature virion
CC (MV) membrane. {ECO:0000250}.
CC -!- PTM: Phosphorylated by viral F10 kinase, phosphorylation state is
CC regulated by H1 phosphatase. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the chordopoxvirinae A14 family. {ECO:0000305}.
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DR EMBL; M35027; AAA48136.1; -; Genomic_DNA.
DR PIR; G42518; G42518.
DR SMR; P20991; -.
DR IntAct; P20991; 1.
DR MINT; P20991; -.
DR Proteomes; UP000008269; Genome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR008785; Poxvirus_A14.
DR Pfam; PF05767; Pox_A14; 1.
PE 3: Inferred from homology;
KW Disulfide bond; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix; Viral envelope protein; Virion.
FT CHAIN 1..90
FT /note="Virion membrane protein A14"
FT /id="PRO_0000099242"
FT TOPO_DOM 1..10
FT /note="Intravirion"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 32..44
FT /note="Virion surface"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 66..90
FT /note="Intravirion"
FT /evidence="ECO:0000255"
FT MOD_RES 85
FT /note="Phosphoserine"
FT /evidence="ECO:0000250"
FT DISULFID 71
FT /note="Interchain"
FT /evidence="ECO:0000250"
SQ SEQUENCE 90 AA; 9994 MW; 98C751EF61678168 CRC64;
MDMMLMIGNY FSGVLIAGII LLILSCIFAF IDFSKSTSPT RTWKVLSIMA FILGIIITVG
MLIYSMWGKH CAPHRVSGVI HTNHSDISMN