MXIA_SHIFL
ID MXIA_SHIFL Reviewed; 686 AA.
AC P0A1I5; P35533; Q55295;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Protein MxiA;
DE AltName: Full=Protein VirH;
GN Name=mxiA; Synonyms=virH; OrderedLocusNames=CP0147;
OS Shigella flexneri.
OG Plasmid pWR100, Plasmid pMYSH6000, and Plasmid pCP301.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a; PLASMID=pWR100;
RX PubMed=1639496; DOI=10.1128/iai.60.8.3287-3295.1992;
RA Andrews G.P., Maurelli A.T.;
RT "mxiA of Shigella flexneri 2a, which facilitates export of invasion plasmid
RT antigens, encodes a homolog of the low-calcium-response protein, LcrD, of
RT Yersinia pestis.";
RL Infect. Immun. 60:3287-3295(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YSH6000 / Serotype 2a; PLASMID=pMYSH6000;
RA Sasakawa C.;
RT "A virulence locus, virH, on the large plasmid of Shigella flexneri.";
RL Submitted (APR-1992) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11115111; DOI=10.1046/j.1365-2958.2000.02179.x;
RA Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H.,
RA Kunst F., Sansonetti P.J., Parsot C.;
RT "The virulence plasmid pWR100 and the repertoire of proteins secreted by
RT the type III secretion apparatus of Shigella flexneri.";
RL Mol. Microbiol. 38:760-771(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11292750; DOI=10.1128/iai.69.5.3271-3285.2001;
RA Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V.,
RA Blattner F.R.;
RT "Complete DNA sequence and analysis of the large virulence plasmid of
RT Shigella flexneri.";
RL Infect. Immun. 69:3271-3285(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a; PLASMID=pCP301;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 489-686.
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=1312536; DOI=10.1128/jb.174.6.1990-2001.1992;
RA Venkatesan M.M., Buysse J.M., Oaks E.V.;
RT "Surface presentation of Shigella flexneri invasion plasmid antigens
RT requires the products of the spa locus.";
RL J. Bacteriol. 174:1990-2001(1992).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 489-686.
RC STRAIN=YSH6000 / Serotype 2a; PLASMID=pMYSH6000;
RX PubMed=8385666; DOI=10.1128/jb.175.8.2334-2346.1993;
RA Sasakawa C., Komatsu K., Tobe T., Suzuki T., Yoshikawa M.;
RT "Eight genes in region 5 that form an operon are essential for invasion of
RT epithelial cells by Shigella flexneri 2a.";
RL J. Bacteriol. 175:2334-2346(1993).
CC -!- FUNCTION: Necessary for the secretion of IPA invasins.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the FHIPEP (flagella/HR/invasion proteins export
CC pore) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA26529.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M91664; AAA26529.1; ALT_INIT; Genomic_DNA.
DR EMBL; D10999; BAA01771.1; -; Genomic_DNA.
DR EMBL; AL391753; CAC05822.1; -; Genomic_DNA.
DR EMBL; AF348706; AAK18466.1; -; Genomic_DNA.
DR EMBL; AF386526; AAL72333.1; -; Genomic_DNA.
DR EMBL; M81458; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; A44797; A44797.
DR RefSeq; NP_085310.1; NC_002698.1.
DR RefSeq; NP_858280.1; NC_004851.1.
DR RefSeq; WP_000616104.1; NZ_UIQL01000031.1.
DR RefSeq; YP_009062504.1; NC_024996.1.
DR PDB; 4A5P; X-ray; 3.15 A; A/B/C=318-686.
DR PDBsum; 4A5P; -.
DR AlphaFoldDB; P0A1I5; -.
DR SMR; P0A1I5; -.
DR STRING; 198214.CP0147; -.
DR EnsemblBacteria; AAL72333; AAL72333; SF_p0147.
DR GeneID; 1238036; -.
DR KEGG; sfl:CP0147; -.
DR PATRIC; fig|198214.7.peg.5394; -.
DR HOGENOM; CLU_015346_3_0_6; -.
DR Proteomes; UP000001006; Plasmid pCP301.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR Gene3D; 1.10.8.540; -; 1.
DR Gene3D; 3.40.30.60; -; 1.
DR Gene3D; 3.40.50.12790; -; 1.
DR InterPro; IPR042194; FHIPEP_1.
DR InterPro; IPR042193; FHIPEP_3.
DR InterPro; IPR042196; FHIPEP_4.
DR InterPro; IPR025505; FHIPEP_CS.
DR InterPro; IPR001712; T3SS_FHIPEP.
DR InterPro; IPR006302; T3SS_HrcV.
DR PANTHER; PTHR30161; PTHR30161; 1.
DR Pfam; PF00771; FHIPEP; 1.
DR PIRSF; PIRSF005419; FlhA; 1.
DR PRINTS; PR00949; TYPE3IMAPROT.
DR TIGRFAMs; TIGR01399; hrcV; 1.
DR PROSITE; PS00994; FHIPEP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Membrane; Plasmid;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport; Virulence.
FT CHAIN 1..686
FT /note="Protein MxiA"
FT /id="PRO_0000190032"
FT TRANSMEM 28..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 95
FT /note="A -> R (in Ref. 1; AAA26529)"
FT /evidence="ECO:0000305"
FT CONFLICT 255..256
FT /note="IV -> ML (in Ref. 1; AAA26529)"
FT /evidence="ECO:0000305"
FT CONFLICT 342
FT /note="S -> T (in Ref. 1; AAA26529)"
FT /evidence="ECO:0000305"
FT CONFLICT 355
FT /note="A -> R (in Ref. 1; AAA26529)"
FT /evidence="ECO:0000305"
FT STRAND 362..368
FT /evidence="ECO:0007829|PDB:4A5P"
FT HELIX 377..391
FT /evidence="ECO:0007829|PDB:4A5P"
FT STRAND 400..406
FT /evidence="ECO:0007829|PDB:4A5P"
FT STRAND 411..416
FT /evidence="ECO:0007829|PDB:4A5P"
FT STRAND 419..425
FT /evidence="ECO:0007829|PDB:4A5P"
FT STRAND 431..433
FT /evidence="ECO:0007829|PDB:4A5P"
FT TURN 442..444
FT /evidence="ECO:0007829|PDB:4A5P"
FT STRAND 448..452
FT /evidence="ECO:0007829|PDB:4A5P"
FT STRAND 455..459
FT /evidence="ECO:0007829|PDB:4A5P"
FT HELIX 478..491
FT /evidence="ECO:0007829|PDB:4A5P"
FT HELIX 494..496
FT /evidence="ECO:0007829|PDB:4A5P"
FT HELIX 500..511
FT /evidence="ECO:0007829|PDB:4A5P"
FT HELIX 515..518
FT /evidence="ECO:0007829|PDB:4A5P"
FT TURN 522..524
FT /evidence="ECO:0007829|PDB:4A5P"
FT HELIX 527..538
FT /evidence="ECO:0007829|PDB:4A5P"
FT TURN 539..541
FT /evidence="ECO:0007829|PDB:4A5P"
FT HELIX 547..557
FT /evidence="ECO:0007829|PDB:4A5P"
FT TURN 558..560
FT /evidence="ECO:0007829|PDB:4A5P"
FT HELIX 564..574
FT /evidence="ECO:0007829|PDB:4A5P"
FT HELIX 576..583
FT /evidence="ECO:0007829|PDB:4A5P"
FT STRAND 591..594
FT /evidence="ECO:0007829|PDB:4A5P"
FT HELIX 596..603
FT /evidence="ECO:0007829|PDB:4A5P"
FT HELIX 623..637
FT /evidence="ECO:0007829|PDB:4A5P"
FT STRAND 644..647
FT /evidence="ECO:0007829|PDB:4A5P"
FT TURN 649..651
FT /evidence="ECO:0007829|PDB:4A5P"
FT HELIX 652..655
FT /evidence="ECO:0007829|PDB:4A5P"
FT TURN 658..662
FT /evidence="ECO:0007829|PDB:4A5P"
FT STRAND 668..670
FT /evidence="ECO:0007829|PDB:4A5P"
FT STRAND 684..686
FT /evidence="ECO:0007829|PDB:4A5P"
SQ SEQUENCE 686 AA; 76145 MW; BD675C2C3BED820F CRC64;
MIQSFLKQVS TKPELIILVL MVMIIAMLII PLPTYLVDFL IGLNIVLAIL VFMGSFYIER
ILSFSTFPSV LLITTLFRLA LSISTSRLIL VDADAGKIIT TFGQFVIGDS LAVGFVIFSI
VTVVQFIVIT KGSERVAEVA ARFSLDGMPG KQMSIDADLK AGIIDAAGAK ERRSILERES
QLYGSFDGAM KFIKGDAIAG IIIIFVNLIG GISVGMSQHG MSLSGALSTY TILTIGDGLV
SQIPALLISI SAGFIVTRVN GDSDNMGRNI MSQIFGNPFV LIVTSALALA IGMLPGFPFF
VFFLIAVTLT ALFYYKKVVE KEKSLSESDS SGYTGTFDID NSHDSSLAMI ENLDAISSET
VPLILLFAEN KINANDMEGL IERIRSQFFI DYGVRLPTIL YRTSNELKVD DIVLLINEVR
ADSFNIYFDK VCITDENGDI DALGIPVVST SYNERVISWV DVSYTENLTN IDAKIKSAQD
EFYHQLSQAL LNNINEIFGI QETKNMLDQF ENRYPDLLKE VFRHVTIQRI SEVLQRLLGE
NISVRNLKLI MESLALWAPR EKDVITLVEH VRASLSRYIC SKIAVSGEIK VVMLSGYIED
AIRKGIRQTS GGSFLNMDIE VSDEVMETLA HALRELRNAK KNFVLLVSVD IRRFVKRLID
NRFKSILVIS YAEIDEAYTI NVLKTI
