MXIC_SHIFL
ID MXIC_SHIFL Reviewed; 355 AA.
AC Q04640; Q8VSG9; Q99QB7;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2003, sequence version 2.
DT 25-MAY-2022, entry version 121.
DE RecName: Full=Protein MxiC;
GN Name=mxiC; OrderedLocusNames=CP0146;
OS Shigella flexneri.
OG Plasmid pWR100, and Plasmid pCP301.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11115111; DOI=10.1046/j.1365-2958.2000.02179.x;
RA Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H.,
RA Kunst F., Sansonetti P.J., Parsot C.;
RT "The virulence plasmid pWR100 and the repertoire of proteins secreted by
RT the type III secretion apparatus of Shigella flexneri.";
RL Mol. Microbiol. 38:760-771(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11292750; DOI=10.1128/iai.69.5.3271-3285.2001;
RA Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V.,
RA Blattner F.R.;
RT "Complete DNA sequence and analysis of the large virulence plasmid of
RT Shigella flexneri.";
RL Infect. Immun. 69:3271-3285(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a; PLASMID=pCP301;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-92.
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=8437520; DOI=10.1111/j.1365-2958.1993.tb01097.x;
RA Allaoui A., Sansonetti P.J., Parsot C.;
RT "MxiD, an outer membrane protein necessary for the secretion of the
RT Shigella flexneri lpa invasins.";
RL Mol. Microbiol. 7:59-68(1993).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 266-355.
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a; PLASMID=pWR100;
RX PubMed=1639496; DOI=10.1128/iai.60.8.3287-3295.1992;
RA Andrews G.P., Maurelli A.T.;
RT "mxiA of Shigella flexneri 2a, which facilitates export of invasion plasmid
RT antigens, encodes a homolog of the low-calcium-response protein, LcrD, of
RT Yersinia pestis.";
RL Infect. Immun. 60:3287-3295(1992).
CC -!- FUNCTION: Necessary for the secretion of IPA invasins.
CC -!- INTERACTION:
CC Q04640; Q6XVW8: spa47; NbExp=3; IntAct=EBI-6405310, EBI-975017;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11115111}. Host cell
CC {ECO:0000305|PubMed:11115111}. Note=Secreted via Mxi-Spa type III
CC secretion system (TTSS), and delivered into the host cytoplasm.
CC {ECO:0000305}.
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DR EMBL; AL391753; CAC05821.1; -; Genomic_DNA.
DR EMBL; AF348706; AAK18465.1; -; Genomic_DNA.
DR EMBL; AF386526; AAL72332.1; -; Genomic_DNA.
DR EMBL; X67206; CAA47645.1; -; Genomic_DNA.
DR EMBL; M91664; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; B44797; B44797.
DR PIR; S28069; S28069.
DR RefSeq; NP_085309.1; NC_002698.1.
DR RefSeq; NP_858279.1; NC_004851.1.
DR RefSeq; WP_010921674.1; NZ_QWST01000007.1.
DR RefSeq; YP_009062503.1; NC_024996.1.
DR PDB; 2VIX; X-ray; 2.85 A; A/B/C=74-355.
DR PDB; 2VJ4; X-ray; 2.50 A; A/B=74-355.
DR PDB; 2VJ5; X-ray; 3.00 A; A/B=74-355.
DR PDBsum; 2VIX; -.
DR PDBsum; 2VJ4; -.
DR PDBsum; 2VJ5; -.
DR AlphaFoldDB; Q04640; -.
DR SMR; Q04640; -.
DR IntAct; Q04640; 1.
DR STRING; 198214.CP0146; -.
DR EnsemblBacteria; AAL72332; AAL72332; SF_p0146.
DR GeneID; 1238035; -.
DR KEGG; sfl:CP0146; -.
DR PATRIC; fig|198214.7.peg.5393; -.
DR HOGENOM; CLU_056140_1_0_6; -.
DR EvolutionaryTrace; Q04640; -.
DR Proteomes; UP000001006; Plasmid pCP301.
DR GO; GO:0009986; C:cell surface; IEA:InterPro.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0043657; C:host cell; IEA:UniProtKB-SubCell.
DR GO; GO:0019867; C:outer membrane; IEA:InterPro.
DR GO; GO:0050709; P:negative regulation of protein secretion; IMP:CACAO.
DR GO; GO:0030254; P:protein secretion by the type III secretion system; IEA:InterPro.
DR InterPro; IPR010812; HrpJ-like.
DR InterPro; IPR003520; Invas_InvE.
DR InterPro; IPR013401; T3SS_LcrE.
DR Pfam; PF07201; HrpJ; 1.
DR PRINTS; PR01344; INVEPROTEIN.
DR TIGRFAMs; TIGR02568; LcrE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Plasmid; Reference proteome;
KW Secreted; Transport; Virulence.
FT CHAIN 1..355
FT /note="Protein MxiC"
FT /id="PRO_0000096656"
FT VARIANT 17
FT /note="R -> K (in plasmid pCP301)"
FT HELIX 75..77
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 83..88
FT /evidence="ECO:0007829|PDB:2VJ4"
FT TURN 91..93
FT /evidence="ECO:0007829|PDB:2VJ4"
FT TURN 105..107
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 108..111
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 118..128
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 138..143
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 146..150
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 157..162
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 164..167
FT /evidence="ECO:0007829|PDB:2VJ4"
FT TURN 170..172
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 178..190
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 195..206
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 208..210
FT /evidence="ECO:0007829|PDB:2VIX"
FT HELIX 211..227
FT /evidence="ECO:0007829|PDB:2VJ4"
FT STRAND 228..230
FT /evidence="ECO:0007829|PDB:2VJ5"
FT HELIX 235..243
FT /evidence="ECO:0007829|PDB:2VJ4"
FT TURN 246..248
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 251..259
FT /evidence="ECO:0007829|PDB:2VJ4"
FT TURN 263..268
FT /evidence="ECO:0007829|PDB:2VJ4"
FT STRAND 270..273
FT /evidence="ECO:0007829|PDB:2VJ5"
FT HELIX 275..287
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 289..291
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 292..295
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 297..303
FT /evidence="ECO:0007829|PDB:2VJ4"
FT TURN 304..306
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 309..321
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 325..327
FT /evidence="ECO:0007829|PDB:2VJ4"
FT HELIX 331..349
FT /evidence="ECO:0007829|PDB:2VJ4"
FT TURN 350..354
FT /evidence="ECO:0007829|PDB:2VJ5"
SQ SEQUENCE 355 AA; 40219 MW; 7F2FDD0734DE741D CRC64;
MLDVKNTGVF SSAFIDRLNA MTNSDDGDET ADAELDSGLA NSKYIDSSDE MASALSSFIN
RRDLEKLKGT NSDSQERILD GEEDEINHKI FDLKRTLKDN LPLDRDFIDR LKRYFKDPSD
QVLALRELLN EKDLTAEQVE LLTKIINEII SGSEKSVNAG INSAIQAKLF GNKMKLEPQL
LRACYRGFIM GNISTTDQYI EWLGNFGFNH RHTIVNFVEQ SLIVDMDSEK PSCNAYEFGF
VLSKLIAIKM IRTSDVIFMK KLESSSLLKD GSLSAEQLLL TLLYIFQYPS ESEQILTSVI
EVSRASHEDS VVYQTYLSSV NESPHDIFKS ESEREIAINI LRELVTSAYK KELSR
