ID   MXIE_SHIFL              Reviewed;         210 AA.
AC   P0A2S7; Q04642; Q55292;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Transcriptional regulator MxiE;
GN   Name=mxiE; OrderedLocusNames=CP0144;
OS   Shigella flexneri.
OG   Plasmid pWR100, Plasmid pINV_F6_M1382, and Plasmid pCP301.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX   PubMed=8437520; DOI=10.1111/j.1365-2958.1993.tb01097.x;
RA   Allaoui A., Sansonetti P.J., Parsot C.;
RT   "MxiD, an outer membrane protein necessary for the secretion of the
RT   Shigella flexneri lpa invasins.";
RL   Mol. Microbiol. 7:59-68(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX   PubMed=11115111; DOI=10.1046/j.1365-2958.2000.02179.x;
RA   Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H.,
RA   Kunst F., Sansonetti P.J., Parsot C.;
RT   "The virulence plasmid pWR100 and the repertoire of proteins secreted by
RT   the type III secretion apparatus of Shigella flexneri.";
RL   Mol. Microbiol. 38:760-771(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX   PubMed=11292750; DOI=10.1128/iai.69.5.3271-3285.2001;
RA   Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V.,
RA   Blattner F.R.;
RT   "Complete DNA sequence and analysis of the large virulence plasmid of
RT   Shigella flexneri.";
RL   Infect. Immun. 69:3271-3285(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=M1382 / Serotype 6; PLASMID=pINV_F6_M1382;
RX   PubMed=14573649; DOI=10.1128/iai.71.11.6298-6306.2003;
RA   Lan R., Stevenson G., Reeves P.R.;
RT   "Comparison of two major forms of the Shigella virulence plasmid pINV:
RT   positive selection is a major force driving the divergence.";
RL   Infect. Immun. 71:6298-6306(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a; PLASMID=pCP301;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
CC   -!- FUNCTION: Necessary for the secretion of ipa invasins. Probable
CC       transcriptional regulatory protein.
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DR   EMBL; X67206; CAA47643.1; -; Genomic_DNA.
DR   EMBL; AL391753; CAC05819.1; -; Genomic_DNA.
DR   EMBL; AF348706; AAK18463.1; -; Genomic_DNA.
DR   EMBL; AY206439; AAP79007.1; -; Genomic_DNA.
DR   EMBL; AF386526; AAL72330.1; -; Genomic_DNA.
DR   PIR; S28067; S28067.
DR   RefSeq; NP_085307.1; NC_002698.1.
DR   RefSeq; NP_858277.1; NC_004851.1.
DR   RefSeq; WP_000398259.1; NZ_WPGS01000043.1.
DR   RefSeq; YP_009062501.1; NC_024996.1.
DR   AlphaFoldDB; P0A2S7; -.
DR   SMR; P0A2S7; -.
DR   IntAct; P0A2S7; 1.
DR   STRING; 198214.CP0144; -.
DR   EnsemblBacteria; AAL72330; AAL72330; SF_p0144.
DR   GeneID; 1238033; -.
DR   KEGG; sfl:CP0144; -.
DR   PATRIC; fig|623.157.peg.5378; -.
DR   HOGENOM; CLU_082966_1_0_6; -.
DR   OMA; LESYWLA; -.
DR   Proteomes; UP000001006; Plasmid pCP301.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR018062; HTH_AraC-typ_CS.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   4: Predicted;
KW   DNA-binding; Plasmid; Reference proteome; Transcription;
KW   Transcription regulation; Transport; Virulence.
FT   CHAIN           1..210
FT                   /note="Transcriptional regulator MxiE"
FT                   /id="PRO_0000194541"
FT   DOMAIN          99..199
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        118..139
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        166..189
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   CONFLICT        176
FT                   /note="A -> R (in Ref. 1; CAA47643)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   210 AA;  24552 MW;  84AA9268852A47DF CRC64;
     MEGFFFVRNQ NIKFSDNVNY HYRFNINSCA KFLAFWDYFS GALVEHSHAE KCIHFYHEND
     LRDSCNTESM LDKLMLRFIF SSDQNVSNAL AMIRMTESYH LVLYLLRTIE KEKEVRIKSL
     TEHYGVSEAY FRSLCRKALG AKVKEQLNTW RLVNGLLDVF LHNQTITSAA MNNGYASTSH
     FSNEIKTRLG FSARELSNIT FLVKKINEKI