MXIG_SHIFL
ID MXIG_SHIFL Reviewed; 371 AA.
AC P0A221; Q57332;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Protein MxiG;
GN Name=mxiG; OrderedLocusNames=CP0136;
OS Shigella flexneri.
OG Plasmid pWR100, and Plasmid pCP301.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION,
RP DISRUPTION PHENOTYPE, AND MUTAGENESIS OF GLY-279.
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=8559065; DOI=10.1111/j.1365-2958.1995.mmi_17030461.x;
RA Allaoui A., Sansonetti P.J., Menard R., Barzu S., Mounier J., Phalipon A.,
RA Parsot C.;
RT "MxiG, a membrane protein required for secretion of Shigella spp. Ipa
RT invasins: involvement in entry into epithelial cells and in intercellular
RT dissemination.";
RL Mol. Microbiol. 17:461-470(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11115111; DOI=10.1046/j.1365-2958.2000.02179.x;
RA Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H.,
RA Kunst F., Sansonetti P.J., Parsot C.;
RT "The virulence plasmid pWR100 and the repertoire of proteins secreted by
RT the type III secretion apparatus of Shigella flexneri.";
RL Mol. Microbiol. 38:760-771(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11292750; DOI=10.1128/iai.69.5.3271-3285.2001;
RA Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V.,
RA Blattner F.R.;
RT "Complete DNA sequence and analysis of the large virulence plasmid of
RT Shigella flexneri.";
RL Infect. Immun. 69:3271-3285(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a; PLASMID=pCP301;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
CC -!- FUNCTION: Involved in the secretion of the Ipa antigens. Involved in
CC the intracellular dissemination of Shigella. Part of the Mxi-Spa
CC secretion apparatus. {ECO:0000269|PubMed:8559065}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:8559065};
CC Single-pass type II membrane protein {ECO:0000269|PubMed:8559065}. Cell
CC outer membrane {ECO:0000269|PubMed:8559065}; Single-pass type II
CC membrane protein {ECO:0000269|PubMed:8559065}.
CC -!- DISRUPTION PHENOTYPE: Loss of secretion of Ipa antigens, and thus
CC unable to enter HeLa cells. {ECO:0000269|PubMed:8559065}.
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DR EMBL; Z48957; CAA88822.1; -; Genomic_DNA.
DR EMBL; AL391753; CAC05811.1; -; Genomic_DNA.
DR EMBL; AF348706; AAK18455.1; -; Genomic_DNA.
DR EMBL; AF386526; AAL72329.1; -; Genomic_DNA.
DR PIR; S70728; S70728.
DR RefSeq; NP_085299.1; NC_002698.1.
DR RefSeq; NP_858269.1; NC_004851.1.
DR RefSeq; WP_001287353.1; NZ_WPGS01000043.1.
DR RefSeq; YP_009062493.1; NC_024996.1.
DR PDB; 2XXS; NMR; -; A=6-112.
DR PDB; 4A4Y; X-ray; 1.57 A; A=1-126.
DR PDB; 6RWK; EM; 3.86 A; A/B/D/E/G/H/J/K/M/N/P/Q/S/T/U/V=1-371.
DR PDB; 6RWX; EM; 3.55 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X=1-371.
DR PDBsum; 2XXS; -.
DR PDBsum; 4A4Y; -.
DR PDBsum; 6RWK; -.
DR PDBsum; 6RWX; -.
DR AlphaFoldDB; P0A221; -.
DR BMRB; P0A221; -.
DR SMR; P0A221; -.
DR IntAct; P0A221; 2.
DR STRING; 198214.CP0136; -.
DR EnsemblBacteria; AAL72329; AAL72329; SF_p0136.
DR GeneID; 1238032; -.
DR KEGG; sfl:CP0136; -.
DR PATRIC; fig|198214.7.peg.5389; -.
DR HOGENOM; CLU_745759_0_0_6; -.
DR OMA; HIQFSIS; -.
DR Proteomes; UP000001006; Plasmid pCP301.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR019029; T3SS_PrgH/EprH-like.
DR Pfam; PF09480; PrgH; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Cell outer membrane;
KW Membrane; Plasmid; Reference proteome; Transmembrane; Transmembrane helix;
KW Virulence.
FT CHAIN 1..371
FT /note="Protein MxiG"
FT /id="PRO_0000096657"
FT TRANSMEM 127..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 279
FT /note="G->A: Defective in intercellular dispersion, however
FT secretes Ipa proteins and enters HeLa cells normally."
FT /evidence="ECO:0000269|PubMed:8559065"
FT STRAND 9..17
FT /evidence="ECO:0007829|PDB:4A4Y"
FT STRAND 23..37
FT /evidence="ECO:0007829|PDB:4A4Y"
FT HELIX 38..43
FT /evidence="ECO:0007829|PDB:4A4Y"
FT STRAND 53..58
FT /evidence="ECO:0007829|PDB:4A4Y"
FT STRAND 65..71
FT /evidence="ECO:0007829|PDB:4A4Y"
FT TURN 73..75
FT /evidence="ECO:0007829|PDB:2XXS"
FT STRAND 77..80
FT /evidence="ECO:0007829|PDB:4A4Y"
FT TURN 82..84
FT /evidence="ECO:0007829|PDB:2XXS"
FT STRAND 91..98
FT /evidence="ECO:0007829|PDB:4A4Y"
FT STRAND 101..107
FT /evidence="ECO:0007829|PDB:4A4Y"
FT STRAND 116..118
FT /evidence="ECO:0007829|PDB:4A4Y"
FT STRAND 121..123
FT /evidence="ECO:0007829|PDB:4A4Y"
SQ SEQUENCE 371 AA; 43002 MW; 9B137FE9A955C3E6 CRC64;
MSEAKNSNLA PFRLLVKLTN GVGDEFPLYY GNNLIVLGRT IETLEFGNDN FPENIIPVTD
SKSDGIIYLT ISKDNICQFS DEKGEQIDIN SQFNSFEYDG ISFHLKNMRE DKSRGHILNG
MYKNHSVFFF FAVIVVLIII FSLSLKKDEV KEIAEIIDDK RYGIVNTGQC NYILAETQND
AVWASVALNK TGFTKCRYIL VSNKEINRIQ QYINQRFPFI NLYVLNLVSD KAELLVFLSK
ERNSSKDTEL DKLKNALIVE FPYIKNIKFN YLSDHNARGD AKGIFTKVNV QYKEICENNK
VTYSVREELT DEKLELINRL ISEHKNIYGD QYIEFSVLLI DDDFKGKSYL NSKDSYVMLN
DKHWFFLDKN K
