ID   MX_STRP9                Reviewed;         369 AA.
AC   P16946;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Virulence factor-related M protein;
DE   Flags: Precursor;
GN   Name=ennX;
OS   Streptococcus pyogenes serotype M49.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=301452;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2687231; DOI=10.1128/jb.171.12.6397-6408.1989;
RA   Haanes E.J., Cleary P.P.;
RT   "Identification of a divergent M protein gene and an M protein-related gene
RT   family in Streptococcus pyogenes serotype 49.";
RL   J. Bacteriol. 171:6397-6408(1989).
CC   -!- INTERACTION:
CC       P16946; P08603: CFH; Xeno; NbExp=2; IntAct=EBI-6403567, EBI-1223708;
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000255|PROSITE-
CC       ProRule:PRU00477}; Peptidoglycan-anchor {ECO:0000255|PROSITE-
CC       ProRule:PRU00477}.
CC   -!- SIMILARITY: Belongs to the M protein family. {ECO:0000305}.
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DR   EMBL; M23689; AAA26919.1; -; Genomic_DNA.
DR   EMBL; M31790; AAA26875.1; -; Genomic_DNA.
DR   PIR; B43715; B43715.
DR   AlphaFoldDB; P16946; -.
DR   SMR; P16946; -.
DR   IntAct; P16946; 1.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0006909; P:phagocytosis; IEA:UniProtKB-KW.
DR   InterPro; IPR019931; LPXTG_anchor.
DR   InterPro; IPR019950; M_anchor.
DR   InterPro; IPR003345; M_repeat.
DR   InterPro; IPR005877; YSIRK_signal_dom.
DR   Pfam; PF00746; Gram_pos_anchor; 1.
DR   Pfam; PF02370; M; 2.
DR   Pfam; PF04650; YSIRK_signal; 1.
DR   PRINTS; PR00015; GPOSANCHOR.
DR   TIGRFAMs; TIGR01168; YSIRK_signal; 1.
DR   PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
PE   1: Evidence at protein level;
KW   Cell wall; Coiled coil; Peptidoglycan-anchor; Phagocytosis; Repeat;
KW   Secreted; Signal; Virulence.
FT   SIGNAL          1..41
FT   CHAIN           42..339
FT                   /note="Virulence factor-related M protein"
FT                   /id="PRO_0000005627"
FT   PROPEP          340..369
FT                   /note="Removed by sortase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT                   /id="PRO_0000005628"
FT   REPEAT          129..159
FT                   /note="A-1"
FT   REPEAT          132..148
FT                   /note="B-1"
FT   REPEAT          150..160
FT                   /note="C-1"
FT   REPEAT          171..200
FT                   /note="A-2"
FT   REPEAT          173..190
FT                   /note="B-2"
FT   REPEAT          192..201
FT                   /note="C-2"
FT   REPEAT          206..215
FT                   /note="C-3"
FT   REPEAT          224..241
FT                   /note="B-3"
FT   REGION          125..189
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          129..200
FT                   /note="2 X repeats, type A"
FT   REGION          132..241
FT                   /note="3 X repeats, type B"
FT   REGION          150..215
FT                   /note="3 X repeats, type C"
FT   REGION          202..271
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          292..341
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           336..340
FT                   /note="LPXTG sorting signal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT   COMPBIAS        125..165
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        202..240
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        257..271
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        315..341
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         339
FT                   /note="Pentaglycyl murein peptidoglycan amidated threonine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
SQ   SEQUENCE   369 AA;  40639 MW;  4AD6D5A72F637839 CRC64;
     MARQQTKKNY SLRKLKTGTA SVAVALTVLG AGFANQTEVR AEGVNATTSL TEKAKYDALK
     DENTGLRGDQ TKLVKKLEEE QEKSKNLEKE KQKLENQALN FQDVIETQEK EKEDLKTTLA
     KATKENEISE ASRKGLSRDL EASRAAKKEL EAKHQKLEAE NKKLTEANQV SEASRKGLSN
     DLEASRAAKK ELEAKYQKLE TDHQALEAKH QKLEADLPKF QRPSRKGLSR DLEASREANK
     KVTSELTQAK AQLSALEESK KLSEKEKAEL QAKLDAQGKA LKEQLAKQTE ELAKLRAEKA
     AGSKTPATKP ANKERSGRAA QTATRPSQNK GMRSQLPSTG EAANPFFTAA AATVMVSAGM
     LALKRKEEN