MY111_ARATH
ID MY111_ARATH Reviewed; 342 AA.
AC Q9FJ07;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Transcription factor MYB111 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Myb-related protein 111 {ECO:0000303|PubMed:11597504};
DE Short=AtMYB111 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Protein PRODUCTION OF FLAVONOL GLYCOSIDES 3 {ECO:0000303|PubMed:17419845};
GN Name=MYB111 {ECO:0000303|PubMed:11597504};
GN Synonyms=PFG3 {ECO:0000303|PubMed:17419845};
GN OrderedLocusNames=At5g49330 {ECO:0000312|Araport:AT5G49330};
GN ORFNames=K21P3.23 {ECO:0000312|EMBL:BAB10351.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: A genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT features of the regions of 1,081,958 bp covered by seventeen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:379-391(1998).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=17419845; DOI=10.1111/j.1365-313x.2007.03078.x;
RA Stracke R., Ishihara H., Huep G., Barsch A., Mehrtens F., Niehaus K.,
RA Weisshaar B.;
RT "Differential regulation of closely related R2R3-MYB transcription factors
RT controls flavonol accumulation in different parts of the Arabidopsis
RT thaliana seedling.";
RL Plant J. 50:660-677(2007).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=20731781; DOI=10.1111/j.1469-8137.2010.03421.x;
RA Stracke R., Jahns O., Keck M., Tohge T., Niehaus K., Fernie A.R.,
RA Weisshaar B.;
RT "Analysis of PRODUCTION OF FLAVONOL GLYCOSIDES-dependent flavonol glycoside
RT accumulation in Arabidopsis thaliana plants reveals MYB11-, MYB12- and
RT MYB111-independent flavonol glycoside accumulation.";
RL New Phytol. 188:985-1000(2010).
RN [7]
RP FUNCTION, INDUCTION BY LIGHT AND UV-B, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia, and cv. Wassilewskija;
RX PubMed=19895401; DOI=10.1111/j.1365-3040.2009.02061.x;
RA Stracke R., Favory J.-J., Gruber H., Bartelniewoehner L., Bartels S.,
RA Binkert M., Funk M., Weisshaar B., Ulm R.;
RT "The Arabidopsis bZIP transcription factor HY5 regulates expression of the
RT PFG1/MYB12 gene in response to light and ultraviolet-B radiation.";
RL Plant Cell Environ. 33:88-103(2010).
RN [8]
RP BIOTECHNOLOGY.
RX PubMed=24846090; DOI=10.1038/srep05018;
RA Pandey A., Misra P., Bhambhani S., Bhatia C., Trivedi P.K.;
RT "Expression of Arabidopsis MYB transcription factor, AtMYB111, in tobacco
RT requires light to modulate flavonol content.";
RL Sci. Rep. 4:5018-5018(2014).
CC -!- FUNCTION: Flavonol-specific transcription activator involved in the
CC regulation of several genes of flavonoid biosynthesis. Activates the
CC expression of CHS, CHI, F3H and FLS1. Controls flavonol biosynthesis
CC primarily in cotyledons and leaves (PubMed:17419845, PubMed:20731781).
CC Confers tolerance to UV-B (PubMed:19895401).
CC {ECO:0000269|PubMed:17419845, ECO:0000269|PubMed:19895401,
CC ECO:0000269|PubMed:20731781}.
CC -!- INTERACTION:
CC Q9FJ07; Q84MC7: PYL9; NbExp=3; IntAct=EBI-25528560, EBI-2349513;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000255|PROSITE-ProRule:PRU00768}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, cotyledons and young
CC leaves. {ECO:0000269|PubMed:17419845}.
CC -!- DEVELOPMENTAL STAGE: In seedlings, predominantly expressed in
CC cotyledons. Restricted to the cotyledons and primary leaves.
CC {ECO:0000269|PubMed:17419845}.
CC -!- INDUCTION: Triggered by HY5 in response to light and UV-B.
CC {ECO:0000269|PubMed:19895401}.
CC -!- DISRUPTION PHENOTYPE: Reduced flavonols levels in leaves. The double
CC mutants myb11 myb111 and myb12 myb111 and triple mutant myb11 myb12
CC myb111 accumulate less flavonols in roots, leaves, stems,
CC inflorescence, and siliques (PubMed:20731781). The triple mutant myb11
CC myb12 myb111 is impaired in flavonols biosynthesis and exhibits a
CC reduced UV-B tolerance (PubMed:17419845, PubMed:19895401).
CC {ECO:0000269|PubMed:17419845, ECO:0000269|PubMed:19895401,
CC ECO:0000269|PubMed:20731781}.
CC -!- BIOTECHNOLOGY: Promotes flavonoid biosynthesis in a light-dependent
CC manner when expressed in tobacco (Nicotiana tabacum) through up-
CC regulation of the biosynthetic genes. {ECO:0000269|PubMed:24846090}.
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DR EMBL; AF371977; AAK97396.1; -; mRNA.
DR EMBL; AY519634; AAS10104.1; -; mRNA.
DR EMBL; AB016872; BAB10351.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95797.1; -; Genomic_DNA.
DR RefSeq; NP_199744.1; NM_124310.3.
DR AlphaFoldDB; Q9FJ07; -.
DR SMR; Q9FJ07; -.
DR IntAct; Q9FJ07; 1.
DR STRING; 3702.AT5G49330.1; -.
DR PaxDb; Q9FJ07; -.
DR PRIDE; Q9FJ07; -.
DR EnsemblPlants; AT5G49330.1; AT5G49330.1; AT5G49330.
DR GeneID; 834993; -.
DR Gramene; AT5G49330.1; AT5G49330.1; AT5G49330.
DR KEGG; ath:AT5G49330; -.
DR Araport; AT5G49330; -.
DR TAIR; locus:2155944; AT5G49330.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_028567_6_3_1; -.
DR InParanoid; Q9FJ07; -.
DR OMA; LDWEFQG; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; Q9FJ07; -.
DR PRO; PR:Q9FJ07; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FJ07; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0051555; P:flavonol biosynthetic process; IMP:TAIR.
DR GO; GO:1900386; P:positive regulation of flavonol biosynthetic process; IMP:UniProtKB.
DR GO; GO:0009416; P:response to light stimulus; IEP:UniProtKB.
DR GO; GO:0010224; P:response to UV-B; IMP:UniProtKB.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..342
FT /note="Transcription factor MYB111"
FT /id="PRO_0000438915"
FT DOMAIN 9..61
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 62..116
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 37..61
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 89..112
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 140..169
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 154..161
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 149..166
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 342 AA; 38481 MW; A564A6197D1E4890 CRC64;
MGRAPCCEKI GLKRGRWTAE EDEILTKYIQ TNGEGSWRSL PKKAGLLRCG KSCRLRWINY
LRRDLKRGNI TSDEEEIIVK LHSLLGNRWS LIATHLPGRT DNEIKNYWNS HLSRKIYAFT
AVSGDGHNLL VNDVVLKKSC SSSSGAKNNN KTKKKKKGRT SRSSMKKHKQ MVTASQCFSQ
PKELESDFSE GGQNGNFEGE SLGPYEWLDG ELERLLSSCV WECTSEEAVI GVNDEKVCES
GDNSSCCVNL FEEEQGSETK IGHVGITEVD HDMTVERERE GSFLSSNSNE NNDKDWWVGL
CNSSEVGFGV DEELLDWEFQ GNVTCQSDDL WDLSDIGEIT LE
