MY115_ARATH
ID MY115_ARATH Reviewed; 359 AA.
AC Q1PDP9; A0MFK5; Q6R048; Q9C5Q0; Q9FNE3;
DT 18-JAN-2017, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Transcription factor MYB115 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Myb-related protein 115 {ECO:0000303|PubMed:11597504};
DE Short=AtMYB115 {ECO:0000303|PubMed:11597504};
GN Name=MYB115 {ECO:0000303|PubMed:11597504};
GN OrderedLocusNames=At5g40360 {ECO:0000312|Araport:AT5G40360};
GN ORFNames=MPO12.8 {ECO:0000312|EMBL:BAB11591.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA Qu L.-J.;
RT "The MYB transcription factor superfamily of Arabidopsis: expression
RT analysis and phylogenetic comparison with the rice MYB family.";
RL Plant Mol. Biol. 60:107-124(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia, cv. Landsberg erecta, and cv. Wassilewskija;
RX PubMed=18695688; DOI=10.1038/cr.2008.276;
RA Wang X., Niu Q.-W., Teng C., Li C., Mu J., Chua N.-H., Zuo J.;
RT "Overexpression of PGA37/MYB118 and MYB115 promotes vegetative-to-embryonic
RT transition in Arabidopsis.";
RL Cell Res. 19:224-235(2009).
RN [7]
RP FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY,
RP INDUCTION BY LEC2, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=27681170; DOI=10.1105/tpc.16.00612;
RA Troncoso-Ponce M.A., Barthole G., Tremblais G., To A., Miquel M.,
RA Lepiniec L., Baud S.;
RT "Transcriptional activation of two palmitoyl-ACP delta9 desaturase genes by
RT MYB115 and MYB118 is critical for biosynthesis of omega-7 monounsaturated
RT fatty acid in the endosperm of Arabidopsis seeds.";
RL Plant Cell 28:2666-2682(2016).
CC -!- FUNCTION: Transcription activator that recognizes the motif 5'-TAACGG-
CC 3' in the promoter of target genes (PubMed:27681170). Promotes
CC vegetative-to-embryonic transition and the formation of somatic embryos
CC from root explants in a WUS-independent manner (PubMed:18695688).
CC Together with MYB118, activates the transcription of S-ACP-DES2/AAD2
CC and S-ACP-DES3/AAD3 thus promoting the biosynthesis of omega-7
CC monounsaturated fatty acid in seed endosperm (PubMed:27681170).
CC {ECO:0000269|PubMed:18695688, ECO:0000269|PubMed:27681170}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:27681170}.
CC -!- TISSUE SPECIFICITY: Accumulates in reproductive organs (e.g. flowers
CC and siliques) (PubMed:18695688, PubMed:27681170). Expressed at very low
CC levels in vegetative organs (PubMed:27681170).
CC {ECO:0000269|PubMed:18695688, ECO:0000269|PubMed:27681170}.
CC -!- DEVELOPMENTAL STAGE: Induced at the onset of the maturation phase in
CC the endosperm at low levels in a heterogeneous repartition,
CC particularly in the chalazal endosperm. Also detected in pollen grains.
CC {ECO:0000269|PubMed:27681170}.
CC -!- INDUCTION: Induced by LEC2 but repressed by MYB118.
CC {ECO:0000269|PubMed:27681170}.
CC -!- DISRUPTION PHENOTYPE: Single and myb118 myb115 double mutants do not
CC show apparent developmental abnormalities (PubMed:18695688). The
CC endosperm oil of double mutant myb115 myb118 lacks omega-7 fatty acids
CC (PubMed:27681170). {ECO:0000269|PubMed:18695688,
CC ECO:0000269|PubMed:27681170}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28730.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
CC Sequence=BAB11591.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF334814; AAK25747.2; -; mRNA.
DR EMBL; AY519633; AAS10103.1; -; mRNA.
DR EMBL; AB006702; BAB11591.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002688; AED94537.1; -; Genomic_DNA.
DR EMBL; DQ447019; ABE66206.1; -; mRNA.
DR EMBL; DQ653331; ABK28730.1; ALT_SEQ; mRNA.
DR RefSeq; NP_568581.1; NM_123400.2.
DR AlphaFoldDB; Q1PDP9; -.
DR SMR; Q1PDP9; -.
DR STRING; 3702.AT5G40360.1; -.
DR PaxDb; Q1PDP9; -.
DR PRIDE; Q1PDP9; -.
DR EnsemblPlants; AT5G40360.1; AT5G40360.1; AT5G40360.
DR GeneID; 834034; -.
DR Gramene; AT5G40360.1; AT5G40360.1; AT5G40360.
DR KEGG; ath:AT5G40360; -.
DR Araport; AT5G40360; -.
DR TAIR; locus:2170583; AT5G40360.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_856178_0_0_1; -.
DR InParanoid; Q1PDP9; -.
DR OMA; IGCHEPV; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; Q1PDP9; -.
DR PRO; PR:Q1PDP9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q1PDP9; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IPI:TAIR.
DR GO; GO:0009960; P:endosperm development; IGI:TAIR.
DR GO; GO:0055089; P:fatty acid homeostasis; IMP:UniProtKB.
DR GO; GO:0045723; P:positive regulation of fatty acid biosynthetic process; IGI:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:2001280; P:positive regulation of unsaturated fatty acid biosynthetic process; IMP:UniProtKB.
DR GO; GO:2000014; P:regulation of endosperm development; IMP:UniProtKB.
DR GO; GO:0010439; P:regulation of glucosinolate biosynthetic process; IGI:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0010262; P:somatic embryogenesis; IMP:UniProtKB.
DR GO; GO:0010228; P:vegetative to reproductive phase transition of meristem; IMP:UniProtKB.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..359
FT /note="Transcription factor MYB115"
FT /id="PRO_0000438620"
FT DOMAIN 153..208
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 209..259
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 181..204
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 232..255
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 124
FT /note="V -> A (in Ref. 1; AAK25747)"
FT /evidence="ECO:0000305"
FT CONFLICT 201
FT /note="H -> R (in Ref. 2; AAS10103)"
FT /evidence="ECO:0000305"
FT CONFLICT 312
FT /note="I -> V (in Ref. 1; AAK25747)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 359 AA; 41600 MW; A585178E0BD4F7DA CRC64;
MYHQNLISST PNQNSNPHDW DIQNPLFSIH PSAEIPSKYP FMGITSCPNT NVFEEFQYKI
TNDQNFPTTY NTPFPVISEG ISYNMHDVQE NTMCGYTAHN QGLIIGCHEP VLVHAVVESQ
QFNVPQSEDI NLVSQSERVT EDKVMFKTDH KKKDIIGKGQ WTPTEDELLV RMVKSKGTKN
WTSIAKMFQG RVGKQCRERW HNHLRPNIKK NDWSEEEDQI LIEVHKIVGN KWTEIAKRLP
GRSENIVKNH WNATKRRLHS VRTKRSDAFS PRNNALENYI RSITINNNAL MNREVDSITA
NSEIDSTRCE NIVDEVMNLN LHATTSVYVP EQAVLTWGYD FTKCYEPMDD TWMLMNGWN
