MY120_ARATH
ID MY120_ARATH Reviewed; 523 AA.
AC Q94FL7; N0ACH1; Q9FIA4;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Transcription factor MYB120 {ECO:0000303|PubMed:11597504};
DE AltName: Full=Myb-related protein 120 {ECO:0000303|PubMed:11597504};
DE Short=AtMYB120 {ECO:0000303|PubMed:11597504};
GN Name=MYB120 {ECO:0000312|EMBL:AAK54742.1};
GN OrderedLocusNames=At5g55020 {ECO:0000312|Araport:AT5G55020};
GN ORFNames=K13P22.2 {ECO:0000312|EMBL:BAB10576.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, DISRUPTION PHENOTYPE,
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia; TISSUE=Pistil;
RX PubMed=23791732; DOI=10.1016/j.cub.2013.05.021;
RA Leydon A.R., Beale K.M., Woroniecka K., Castner E., Chen J., Horgan C.,
RA Palanivelu R., Johnson M.A.;
RT "Three MYB transcription factors control pollen tube differentiation
RT required for sperm release.";
RL Curr. Biol. 23:1209-1214(2013).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP INDUCTION BY POLLEN TUBE GERMINATION, AND DISRUPTION PHENOTYPE.
RX PubMed=19714218; DOI=10.1371/journal.pgen.1000621;
RA Qin Y., Leydon A.R., Manziello A., Pandey R., Mount D., Denic S., Vasic B.,
RA Johnson M.A., Palanivelu R.;
RT "Penetration of the stigma and style elicits a novel transcriptome in
RT pollen tubes, pointing to genes critical for growth in a pistil.";
RL PLoS Genet. 5:E1000621-E1000621(2009).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=24278028; DOI=10.1371/journal.pgen.1003933;
RA Liang Y., Tan Z.-M., Zhu L., Niu Q.-K., Zhou J.-J., Li M., Chen L.-Q.,
RA Zhang X.-Q., Ye D.;
RT "MYB97, MYB101 and MYB120 function as male factors that control pollen
RT tube-synergid interaction in Arabidopsis thaliana fertilization.";
RL PLoS Genet. 9:E1003933-E1003933(2013).
CC -!- FUNCTION: Transcription activator (PubMed:24278028). Binds to 5'-
CC CAACTGTC-3' and/or 5'-TAACAAA-3' motif in target gene promoter to
CC promote their expression (By similarity). Together with MYB97 and
CC MYB101, functions as a male factor that controls pollen tube-synergid
CC interaction in fertilization. Required for pollen tube growth arrest
CC and sperm cell release in the female gametophyte, probably via the
CC regulation of pollen tube-specific gene expression (PubMed:24278028,
CC PubMed:23791732). {ECO:0000250|UniProtKB:O80883,
CC ECO:0000269|PubMed:23791732, ECO:0000269|PubMed:24278028}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:23791732, ECO:0000269|PubMed:24278028}.
CC Note=Detected in the vegetative nucleus of pollen tubes.
CC {ECO:0000269|PubMed:23791732}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q94FL7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q94FL7-2; Sequence=VSP_058817;
CC -!- TISSUE SPECIFICITY: Expressed in pollen grains and pollen tube
CC (PubMed:23791732, PubMed:24278028). Mostly expressed in mature pollen
CC grains, and, to a lower extent, in inflorescences and siliques
CC (PubMed:24278028). {ECO:0000269|PubMed:23791732,
CC ECO:0000269|PubMed:24278028}.
CC -!- DEVELOPMENTAL STAGE: Accumulates in the pollen tube nucleus during
CC pollen tube growth through the pistil. {ECO:0000269|PubMed:23791732}.
CC -!- INDUCTION: Accumulates in pollen tube 4 hours after pollen germination.
CC {ECO:0000269|PubMed:19714218}.
CC -!- DISRUPTION PHENOTYPE: Disrupted pollen tube growth (PubMed:19714218).
CC The triple mutant myb97 myb101 myb120 is impaired in pollen tube growth
CC arrest and subsequent sperm cell release in the female gametophyte thus
CC leading to a drastically reduced fertility. Altered pollen tube-
CC specific gene expression (PubMed:23791732, PubMed:24278028).
CC {ECO:0000269|PubMed:19714218, ECO:0000269|PubMed:23791732,
CC ECO:0000269|PubMed:24278028}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB10576.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF371979; AAK54742.1; -; mRNA.
DR EMBL; KC544014; AGK45259.1; -; mRNA.
DR EMBL; AB017059; BAB10576.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED96570.1; -; Genomic_DNA.
DR RefSeq; NP_568819.1; NM_124884.2. [Q94FL7-1]
DR AlphaFoldDB; Q94FL7; -.
DR SMR; Q94FL7; -.
DR STRING; 3702.AT5G55020.1; -.
DR PaxDb; Q94FL7; -.
DR PRIDE; Q94FL7; -.
DR EnsemblPlants; AT5G55020.1; AT5G55020.1; AT5G55020. [Q94FL7-1]
DR GeneID; 835593; -.
DR Gramene; AT5G55020.1; AT5G55020.1; AT5G55020. [Q94FL7-1]
DR KEGG; ath:AT5G55020; -.
DR Araport; AT5G55020; -.
DR TAIR; locus:2151938; AT5G55020.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_023548_0_0_1; -.
DR InParanoid; Q94FL7; -.
DR OMA; YWSSTSG; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; Q94FL7; -.
DR PRO; PR:Q94FL7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q94FL7; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0090406; C:pollen tube; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0048235; P:pollen sperm cell differentiation; IMP:UniProtKB.
DR GO; GO:0009860; P:pollen tube growth; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0080092; P:regulation of pollen tube growth; IMP:UniProtKB.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Repeat; Transcription; Transcription regulation.
FT CHAIN 1..523
FT /note="Transcription factor MYB120"
FT /id="PRO_0000439246"
FT DOMAIN 23..75
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 76..130
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 51..75
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 103..126
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 140..254
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 332..373
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 396..426
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 444..470
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 152..169
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 170..227
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 228..243
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 494..523
FT /note="GKTKTTYNTSNLNYSSLQVKCKMFMIKTQI -> DLKSKEEESLQMNTMQED
FT IAKLLDWGSDSGEISNGQSSVVTDDNLVLDVHQLASLFPADSTAVVAATNDQHNKNNNN
FT NCSWDDMQGIR (in isoform 2)"
FT /id="VSP_058817"
FT CONFLICT 110
FT /note="L -> V (in Ref. 2; AGK45259)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 523 AA; 58217 MW; 5517EE8A00A8CB7E CRC64;
MIMYGGGGAG KDGGSTNHLS DGGVILKKGP WTAAEDEILA AYVRENGEGN WNAVQKNTGL
ARCGKSCRLR WANHLRPNLK KGSFTGDEER LIIQLHAQLG NKWARMAAQL PGRTDNEIKN
YWNTRLKRLL RQGLPLYPPD IIPNHQLHPH PHHQQQQQHN HHHHHHQQQQ QHQQMYFQPQ
SSQRNTPSSS PLPSPTPANA KSSSSFTFHT TTANLLHPLS PHTPNTPSQL SSTPPPPPLS
SPLCSPRNNQ YPTLPLFALP RSQINNNNNG NFTFPRPPPL LQPPSSLFAK RYNNANTPLN
CINRVSTAPF SPVSRDSYTS FLTLPYPSPT AQTATYHNTN NPYSSSPSFS LNPSSSSYPT
STSSPSFLHS HYTPSSTSFH TNPVYSMKQE QLPSNQIPQI DGFNNVNNFT DNERQNHNLN
SSGAHRRSSS CSLLEDVFEE AEALASGGRG RPPKRRQLTA SLPNHNNNTN NNDNFFSVSF
GHYDSSDNLC SLQGKTKTTY NTSNLNYSSL QVKCKMFMIK TQI
