MYB12_ARATH
ID MYB12_ARATH Reviewed; 371 AA.
AC O22264; Q38IY2; Q9SBG6;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Transcription factor MYB12 {ECO:0000303|PubMed:9839469};
DE AltName: Full=Myb-related protein 12 {ECO:0000303|PubMed:9839469};
DE Short=AtMYB12 {ECO:0000303|PubMed:9839469};
DE AltName: Full=Protein PRODUCTION OF FLAVONOL GLYCOSIDES 1 {ECO:0000303|PubMed:17419845};
GN Name=MYB12 {ECO:0000303|PubMed:9839469};
GN Synonyms=PFG1 {ECO:0000303|PubMed:17419845};
GN OrderedLocusNames=At2g47460 {ECO:0000312|Araport:AT2G47460};
GN ORFNames=T30B22.24 {ECO:0000312|EMBL:AAC62864.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION BY SUCROSE;
RP NITROGEN DEFICIENCY AND UV LIGHT, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=9839469; DOI=10.1046/j.1365-313x.1998.00278.x;
RA Kranz H.D., Denekamp M., Greco R., Jin H.-L., Leyva A., Meissner R.C.,
RA Petroni K., Urzainqui A., Bevan M., Martin C., Smeekens S., Tonelli C.,
RA Paz-Ares J., Weisshaar B.;
RT "Towards functional characterisation of the members of the R2R3-MYB gene
RT family from Arabidopsis thaliana.";
RL Plant J. 16:263-276(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=15923334; DOI=10.1104/pp.104.058032;
RA Mehrtens F., Kranz H., Bednarek P., Weisshaar B.;
RT "The Arabidopsis transcription factor MYB12 is a flavonol-specific
RT regulator of phenylpropanoid biosynthesis.";
RL Plant Physiol. 138:1083-1096(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L.-J., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: a genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [8]
RP GENE FAMILY.
RX PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA Qu L.-J.;
RT "The MYB transcription factor superfamily of Arabidopsis: expression
RT analysis and phylogenetic comparison with the rice MYB family.";
RL Plant Mol. Biol. 60:107-124(2006).
RN [9]
RP INDUCTION BY NITROGEN DEFICIENCY.
RX PubMed=17053893; DOI=10.1007/s00425-006-0414-x;
RA Lea U.S., Slimestad R., Smedvig P., Lillo C.;
RT "Nitrogen deficiency enhances expression of specific MYB and bHLH
RT transcription factors and accumulation of end products in the flavonoid
RT pathway.";
RL Planta 225:1245-1253(2007).
RN [10]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Columbia;
RX PubMed=17419845; DOI=10.1111/j.1365-313x.2007.03078.x;
RA Stracke R., Ishihara H., Huep G., Barsch A., Mehrtens F., Niehaus K.,
RA Weisshaar B.;
RT "Differential regulation of closely related R2R3-MYB transcription factors
RT controls flavonol accumulation in different parts of the Arabidopsis
RT thaliana seedling.";
RL Plant J. 50:660-677(2007).
RN [11]
RP BIOTECHNOLOGY.
RX PubMed=18643978; DOI=10.1111/j.1365-313x.2008.03597.x;
RA Luo J., Butelli E., Hill L., Parr A., Niggeweg R., Bailey P., Weisshaar B.,
RA Martin C.;
RT "AtMYB12 regulates caffeoyl quinic acid and flavonol synthesis in tomato:
RT expression in fruit results in very high levels of both types of
RT polyphenol.";
RL Plant J. 56:316-326(2008).
RN [12]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=20731781; DOI=10.1111/j.1469-8137.2010.03421.x;
RA Stracke R., Jahns O., Keck M., Tohge T., Niehaus K., Fernie A.R.,
RA Weisshaar B.;
RT "Analysis of PRODUCTION OF FLAVONOL GLYCOSIDES-dependent flavonol glycoside
RT accumulation in Arabidopsis thaliana plants reveals MYB11-, MYB12- and
RT MYB111-independent flavonol glycoside accumulation.";
RL New Phytol. 188:985-1000(2010).
RN [13]
RP FUNCTION, INDUCTION BY LIGHT AND UV-B, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia, and cv. Wassilewskija;
RX PubMed=19895401; DOI=10.1111/j.1365-3040.2009.02061.x;
RA Stracke R., Favory J.-J., Gruber H., Bartelniewoehner L., Bartels S.,
RA Binkert M., Funk M., Weisshaar B., Ulm R.;
RT "The Arabidopsis bZIP transcription factor HY5 regulates expression of the
RT PFG1/MYB12 gene in response to light and ultraviolet-B radiation.";
RL Plant Cell Environ. 33:88-103(2010).
RN [14]
RP BIOTECHNOLOGY.
RX PubMed=20190095; DOI=10.1104/pp.109.150979;
RA Misra P., Pandey A., Tiwari M., Chandrashekar K., Sidhu O.P., Asif M.H.,
RA Chakrabarty D., Singh P.K., Trivedi P.K., Nath P., Tuli R.;
RT "Modulation of transcriptome and metabolome of tobacco by Arabidopsis
RT transcription factor, AtMYB12, leads to insect resistance.";
RL Plant Physiol. 152:2258-2268(2010).
CC -!- FUNCTION: Flavonol-specific transcription activator involved in the
CC regulation of several genes of flavonoid biosynthesis. Activates the
CC expression of CHS, CHI, F3H and FLS1. Controls flavonol biosynthesis
CC mainly in the root (PubMed:17419845, PubMed:20731781). Confers
CC tolerance to UV-B (PubMed:19895401). {ECO:0000269|PubMed:15923334,
CC ECO:0000269|PubMed:17419845, ECO:0000269|PubMed:19895401,
CC ECO:0000269|PubMed:20731781}.
CC -!- INTERACTION:
CC O22264; Q9FGD2: At5g66980; NbExp=4; IntAct=EBI-15191921, EBI-15191923;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625}.
CC -!- TISSUE SPECIFICITY: Expressed in stems and flower buds
CC (PubMed:9839469). Expressed in seedlings, roots, cotyledons and apical
CC meristems (PubMed:17419845). {ECO:0000269|PubMed:17419845,
CC ECO:0000269|PubMed:9839469}.
CC -!- DEVELOPMENTAL STAGE: In seedlings, predominantly expressed in roots.
CC Expressed predominantly in the root, in the vascular tissue of the
CC hypocotyl-root transition zone and, at low levels, at the region of
CC apical meristem and the apex of cotyledons.
CC {ECO:0000269|PubMed:17419845}.
CC -!- INDUCTION: By nitrogen deficiency, sucrose and UV LIGHT
CC (PubMed:17053893, PubMed:9839469). Triggered by HY5 in response to
CC light and UV-B (PubMed:19895401). {ECO:0000269|PubMed:17053893,
CC ECO:0000269|PubMed:19895401, ECO:0000269|PubMed:9839469}.
CC -!- DISRUPTION PHENOTYPE: Reduced flavonols levels in roots. The double
CC mutant myb12 myb111 and triple mutant myb11 myb12 myb111 accumulate
CC less flavonols in roots, leaves, stems, inflorescence, and siliques.
CC The double mutant myb11 myb12 is specifically altered in flavonols
CC content of siliques and roots (PubMed:20731781). The triple mutant
CC myb11 myb12 myb111 is impaired in flavonols biosynthesis and exhibits a
CC reduced UV-B tolerance (PubMed:17419845, PubMed:19895401).
CC {ECO:0000269|PubMed:17419845, ECO:0000269|PubMed:19895401,
CC ECO:0000269|PubMed:20731781}.
CC -!- BIOTECHNOLOGY: Promotes flavonoid biosynthesis when expressed in
CC tobacco (Nicotiana tabacum) and tomato (Solanum lycopersicum) through
CC up-regulation of the biosynthetic genes. Tobacco plants exhibit
CC increased insect (Spodoptera litura and Helicoverpa armigera)
CC resistance due to enhanced accumulation of rutin.
CC {ECO:0000269|PubMed:18643978, ECO:0000269|PubMed:20190095}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABB03913.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF062864; AAC83586.1; -; mRNA.
DR EMBL; DQ224277; ABB03913.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AY519580; AAS10050.1; -; mRNA.
DR EMBL; AC002535; AAC62864.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10843.1; -; Genomic_DNA.
DR EMBL; AY060588; AAL31213.1; -; mRNA.
DR EMBL; AY142067; AAM98331.1; -; mRNA.
DR PIR; T00438; T00438.
DR PIR; T51636; T51636.
DR RefSeq; NP_182268.1; NM_130314.4.
DR AlphaFoldDB; O22264; -.
DR SMR; O22264; -.
DR BioGRID; 4694; 16.
DR IntAct; O22264; 4.
DR STRING; 3702.AT2G47460.1; -.
DR PaxDb; O22264; -.
DR PRIDE; O22264; -.
DR EnsemblPlants; AT2G47460.1; AT2G47460.1; AT2G47460.
DR GeneID; 819359; -.
DR Gramene; AT2G47460.1; AT2G47460.1; AT2G47460.
DR KEGG; ath:AT2G47460; -.
DR Araport; AT2G47460; -.
DR TAIR; locus:2062040; AT2G47460.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_028567_6_3_1; -.
DR InParanoid; O22264; -.
DR OMA; CPSVESF; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; O22264; -.
DR PRO; PR:O22264; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22264; baseline and differential.
DR Genevisible; O22264; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IMP:TAIR.
DR GO; GO:0051555; P:flavonol biosynthetic process; IMP:UniProtKB.
DR GO; GO:1900386; P:positive regulation of flavonol biosynthetic process; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0009733; P:response to auxin; IEP:TAIR.
DR GO; GO:0009723; P:response to ethylene; IEP:TAIR.
DR GO; GO:0009416; P:response to light stimulus; IEP:UniProtKB.
DR GO; GO:0009651; P:response to salt stress; IMP:TAIR.
DR GO; GO:0010224; P:response to UV-B; IMP:UniProtKB.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Flavonoid biosynthesis; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation.
FT CHAIN 1..371
FT /note="Transcription factor MYB12"
FT /id="PRO_0000285269"
FT DOMAIN 9..61
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 62..116
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 37..61
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 89..112
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 136..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 152..170
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 154
FT /note="S -> G (in Ref. 1; AAC83586)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 371 AA; 41394 MW; 66FA7B8720011947 CRC64;
MGRAPCCEKV GIKRGRWTAE EDQILSNYIQ SNGEGSWRSL PKNAGLKRCG KSCRLRWINY
LRSDLKRGNI TPEEEELVVK LHSTLGNRWS LIAGHLPGRT DNEIKNYWNS HLSRKLHNFI
RKPSISQDVS AVIMTNASSA PPPPQAKRRL GRTSRSAMKP KIHRTKTRKT KKTSAPPEPN
ADVAGADKEA LMVESSGAEA ELGRPCDYYG DDCNKNLMSI NGDNGVLTFD DDIIDLLLDE
SDPGHLYTNT TCGGDGELHN IRDSEGARGF SDTWNQGNLD CLLQSCPSVE SFLNYDHQVN
DASTDEFIDW DCVWQEGSDN NLWHEKENPD SMVSWLLDGD DEATIGNSNC ENFGEPLDHD
DESALVAWLL S
