MYB15_ARATH
ID MYB15_ARATH Reviewed; 285 AA.
AC Q9LTC4; O49744; Q39260;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Transcription factor MYB15 {ECO:0000305};
DE AltName: Full=Myb-related protein 15 {ECO:0000305};
DE Short=AtMYB15 {ECO:0000312|EMBL:CAA74603.1};
DE AltName: Full=Myb-related protein Y19 {ECO:0000305};
DE Short=AtY19 {ECO:0000303|PubMed:8980549};
GN Name=MYB15 {ECO:0000312|EMBL:CAA74603.1};
GN OrderedLocusNames=At3g23250 {ECO:0000312|Araport:AT3G23250};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Romero I., Fuertes A., Benito M.J., Malpica J., Leyva A., Paz-Ares J.;
RT "One hundred R2R3-MYB genes in the genome of Arabidopsis thaliana.";
RL Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: a genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-122.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=8980549; DOI=10.1007/bf00020495;
RA Quaedvlieg N., Dockx J., Keultjes G., Kock P., Wilmering J., Weisbeek P.,
RA Smeekens S.;
RT "Identification of a light-regulated MYB gene from an Arabidopsis
RT transcription factor gene collection.";
RL Plant Mol. Biol. 32:987-993(1996).
RN [8]
RP FUNCTION, INTERACTION WITH SCRM/ICE1, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND INDUCTION BY SALT STRESS.
RX PubMed=17015446; DOI=10.1074/jbc.m605895200;
RA Agarwal M., Hao Y., Kapoor A., Dong C.H., Fujii H., Zheng X., Zhu J.K.;
RT "A R2R3 type MYB transcription factor is involved in the cold regulation of
RT CBF genes and in acquired freezing tolerance.";
RL J. Biol. Chem. 281:37636-37645(2006).
RN [9]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=19161942; DOI=10.1016/s1673-8527(09)60003-5;
RA Ding Z., Li S., An X., Liu X., Qin H., Wang D.;
RT "Transgenic expression of MYB15 confers enhanced sensitivity to abscisic
RT acid and improved drought tolerance in Arabidopsis thaliana.";
RL J. Genet. Genomics 36:17-29(2009).
CC -!- FUNCTION: Transcription factor involved in cold-regulation of CBF genes
CC and in the development of freezing tolerance. May be part of a complex
CC network of transcription factors controlling the expression of CBF
CC genes and other genes in response to cold stress. Binds to the MYB
CC recognition sequences in the promoters of CBF1, CBF2 and CBF3 genes
CC (PubMed:17015446). Involved in drought and salt tolerance. May enhance
CC expression levels of genes involved in abscisic acid (ABA) biosynthesis
CC and signaling, as well as those encoding stress-protective proteins
CC (PubMed:19161942). {ECO:0000269|PubMed:17015446,
CC ECO:0000269|PubMed:19161942}.
CC -!- SUBUNIT: Interacts with SCRM/ICE1. {ECO:0000269|PubMed:17015446}.
CC -!- INTERACTION:
CC Q9LTC4; O24407: IAA16; NbExp=3; IntAct=EBI-21497119, EBI-632231;
CC Q9LTC4; P93830: IAA17; NbExp=3; IntAct=EBI-21497119, EBI-632243;
CC Q9LTC4; O24409: IAA19; NbExp=3; IntAct=EBI-21497119, EBI-632257;
CC Q9LTC4; O24410: IAA20; NbExp=3; IntAct=EBI-21497119, EBI-632272;
CC Q9LTC4; Q8LAL2: IAA26; NbExp=3; IntAct=EBI-21497119, EBI-3947418;
CC Q9LTC4; Q9C5X0: IAA34; NbExp=3; IntAct=EBI-21497119, EBI-3946459;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:17015446}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems and flowers
CC (PubMed:17015446, PubMed:19161942). Expressed in stomatal guard cells
CC (PubMed:19161942). {ECO:0000269|PubMed:17015446,
CC ECO:0000269|PubMed:19161942}.
CC -!- INDUCTION: Induced by abscisic acid (ABA) and drought stress
CC (PubMed:19161942). Induced by salt stress (PubMed:19161942).
CC {ECO:0000269|PubMed:19161942}.
CC -!- MISCELLANEOUS: Plants over-expressing MYB15 have improved drought and
CC salt tolerance. {ECO:0000269|PubMed:19161942}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA74603.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; Y14207; CAA74603.1; ALT_FRAME; mRNA.
DR EMBL; AY550296; AAS58507.1; -; mRNA.
DR EMBL; AB025608; BAA95738.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76740.1; -; Genomic_DNA.
DR EMBL; AK176617; BAD44380.1; -; mRNA.
DR EMBL; AK176693; BAD44456.1; -; mRNA.
DR EMBL; BT026046; ABG48402.1; -; mRNA.
DR EMBL; X90384; CAA62032.1; -; Genomic_DNA.
DR PIR; S58294; S58294.
DR RefSeq; NP_188966.1; NM_113226.5.
DR AlphaFoldDB; Q9LTC4; -.
DR SMR; Q9LTC4; -.
DR IntAct; Q9LTC4; 7.
DR STRING; 3702.AT3G23250.1; -.
DR iPTMnet; Q9LTC4; -.
DR PaxDb; Q9LTC4; -.
DR PRIDE; Q9LTC4; -.
DR ProteomicsDB; 248918; -.
DR EnsemblPlants; AT3G23250.1; AT3G23250.1; AT3G23250.
DR GeneID; 821904; -.
DR Gramene; AT3G23250.1; AT3G23250.1; AT3G23250.
DR KEGG; ath:AT3G23250; -.
DR Araport; AT3G23250; -.
DR TAIR; locus:2086233; AT3G23250.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_028567_6_4_1; -.
DR InParanoid; Q9LTC4; -.
DR OMA; SHEGHSN; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; Q9LTC4; -.
DR PRO; PR:Q9LTC4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LTC4; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0009409; P:response to cold; IDA:UniProtKB.
DR GO; GO:0009651; P:response to salt stress; IMP:UniProtKB.
DR GO; GO:0009414; P:response to water deprivation; IMP:UniProtKB.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Repeat; Stress response;
KW Transcription; Transcription regulation.
FT CHAIN 1..285
FT /note="Transcription factor MYB15"
FT /id="PRO_0000439652"
FT DOMAIN 9..61
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 62..116
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 37..61
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 89..112
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 115..172
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 115..136
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 137..172
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 285 AA; 32042 MW; 51CEF6C6DF50F828 CRC64;
MGRAPCCEKM GLKRGPWTPE EDQILVSFIL NHGHSNWRAL PKQAGLLRCG KSCRLRWMNY
LKPDIKRGNF TKEEEDAIIS LHQILGNRWS AIAAKLPGRT DNEIKNVWHT HLKKRLEDYQ
PAKPKTSNKK KGTKPKSESV ITSSNSTRSE SELADSSNPS GESLFSTSPS TSEVSSMTLI
SHDGYSNEIN MDNKPGDIST IDQECVSFET FGADIDESFW KETLYSQDEH NYVSNDLEVA
GLVEIQQEFQ NLGSANNEMI FDSEMDFWFD VLARTGGEQD LLAGL
