MYB21_ARATH
ID MYB21_ARATH Reviewed; 226 AA.
AC Q9LK95; C0SVD2; O22684; Q9SBG4;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Transcription factor MYB21;
DE AltName: Full=Myb homolog 3;
DE Short=AtMyb3;
DE AltName: Full=Myb-related protein 21;
DE Short=AtMYB21;
GN Name=MYB21; Synonyms=MYB3; OrderedLocusNames=At3g27810; ORFNames=MGF10.23;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RA Noji M., Urao T., Shinozaki K.-Y., Shinozaki K.;
RT "Molecular cloning of two cDNAs encoding novel myb homologs from
RT Arabidopsis.";
RL (er) Plant Gene Register PGR98-111(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=15208423; DOI=10.1104/pp.104.042176;
RA Gong W., Shen Y.-P., Ma L.-G., Pan Y., Du Y.-L., Wang D.-H., Yang J.-Y.,
RA Hu L.-D., Liu X.-F., Dong C.-X., Ma L., Chen Y.-H., Yang X.-Y., Gao Y.,
RA Zhu D., Tan X., Mu J.-Y., Zhang D.-B., Liu Y.-L., Dinesh-Kumar S.P., Li Y.,
RA Wang X.-P., Gu H.-Y., Qu L.-J., Bai S.-N., Lu Y.-T., Li J.-Y., Zhao J.-D.,
RA Zuo J., Huang H., Deng X.-W., Zhu Y.-X.;
RT "Genome-wide ORFeome cloning and analysis of Arabidopsis transcription
RT factor genes.";
RL Plant Physiol. 135:773-782(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 86-226, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=9839469; DOI=10.1046/j.1365-313x.1998.00278.x;
RA Kranz H.D., Denekamp M., Greco R., Jin H.-L., Leyva A., Meissner R.C.,
RA Petroni K., Urzainqui A., Bevan M., Martin C., Smeekens S., Tonelli C.,
RA Paz-Ares J., Weisshaar B.;
RT "Towards functional characterisation of the members of the R2R3-MYB gene
RT family from Arabidopsis thaliana.";
RL Plant J. 16:263-276(1998).
RN [9]
RP FUNCTION, AND PROBABLE UBIQUITINATION BY COP1.
RX PubMed=11967090; DOI=10.1046/j.1365-313x.2002.01264.x;
RA Shin B., Choi G., Yi H., Yang S., Cho I., Kim J., Lee S., Paek N.-C.,
RA Kim J.-H., Song P.S., Choi G.;
RT "AtMYB21, a gene encoding a flower-specific transcription factor, is
RT regulated by COP1.";
RL Plant J. 30:23-32(2002).
RN [10]
RP GENE FAMILY.
RX PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA Qu L.-J.;
RT "The MYB transcription factor superfamily of Arabidopsis: expression
RT analysis and phylogenetic comparison with the rice MYB family.";
RL Plant Mol. Biol. 60:107-124(2006).
RN [11]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY JASMONATE, AND TISSUE
RP SPECIFICITY.
RX PubMed=16805732; DOI=10.1111/j.1365-313x.2006.02756.x;
RA Mandaokar A., Thines B., Shin B., Lange B.M., Choi G., Koo Y.J., Yoo Y.J.,
RA Choi Y.D., Choi G., Browse J.;
RT "Transcriptional regulators of stamen development in Arabidopsis identified
RT by transcriptional profiling.";
RL Plant J. 46:984-1008(2006).
RN [12]
RP FUNCTION.
RX PubMed=19091873; DOI=10.1104/pp.108.132597;
RA Mandaokar A., Browse J.;
RT "MYB108 acts together with MYB24 to regulate jasmonate-mediated stamen
RT maturation in Arabidopsis.";
RL Plant Physiol. 149:851-862(2009).
RN [13]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=19325888; DOI=10.1371/journal.pgen.1000440;
RA Cheng H., Song S., Xiao L., Soo H.M., Cheng Z., Xie D., Peng J.;
RT "Gibberellin acts through jasmonate to control the expression of MYB21,
RT MYB24, and MYB57 to promote stamen filament growth in Arabidopsis.";
RL PLoS Genet. 5:E1000440-E1000440(2009).
RN [14]
RP FUNCTION, AND INTERACTION WITH TIFY10A/JAZ1; TIFY5A/JAZ8 AND TIFY3A/JAZ11.
RX PubMed=21447791; DOI=10.1105/tpc.111.083089;
RA Song S., Qi T., Huang H., Ren Q., Wu D., Chang C., Peng W., Liu Y.,
RA Peng J., Xie D.;
RT "The Jasmonate-ZIM domain proteins interact with the R2R3-MYB transcription
RT factors MYB21 and MYB24 to affect Jasmonate-regulated stamen development in
RT Arabidopsis.";
RL Plant Cell 23:1000-1013(2011).
CC -!- FUNCTION: Transcription factor involved in photomorphogenesis in the
CC light. May act downstream of the light receptor network and directly
CC affects transcription of light-induced genes. In darkness, its probable
CC degradation prevent the activation of light-induced genes. Required to
CC activate expression of PAL. Acts redundantly with MYB24 and MYB57 to
CC control stamen filament elongation in the late developed flowers.
CC Contributes with MYB24 to induction of MYB108 by jasmonate. Repressed
CC at the transcript levels by DELLA proteins.
CC {ECO:0000269|PubMed:11967090, ECO:0000269|PubMed:16805732,
CC ECO:0000269|PubMed:19091873, ECO:0000269|PubMed:19325888,
CC ECO:0000269|PubMed:21447791}.
CC -!- SUBUNIT: Interacts (via N-terminus) with TIFY10A/JAZ1, TIFY5A/JAZ8 AND
CC TIFY3A/JAZ11. {ECO:0000269|PubMed:21447791}.
CC -!- INTERACTION:
CC Q9LK95; Q8S307: BZR1; NbExp=3; IntAct=EBI-15197631, EBI-1803261;
CC Q9LK95; Q94ID6: ERF12; NbExp=3; IntAct=EBI-15197631, EBI-4446727;
CC Q9LK95; Q9MAI5: ERF8; NbExp=3; IntAct=EBI-15197631, EBI-2000137;
CC Q9LK95; Q9LUA3: NIMIN-2; NbExp=3; IntAct=EBI-15197631, EBI-541107;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Mainly expressed in floral tissues. expressed in
CC all four whorls of the flower, in the anther vascular tissue and in
CC cells at the junction between anther and stamen filaments. Detected in
CC the nectaries and ovules. {ECO:0000269|PubMed:16805732,
CC ECO:0000269|PubMed:19325888, ECO:0000269|Ref.1}.
CC -!- INDUCTION: Up-regulated by jasmonate. {ECO:0000269|PubMed:16805732}.
CC -!- PTM: Probably ubiquitinated by COP1. Ubiquitination takes place in
CC darkness and leads to its subsequent degradation, thereby preventing to
CC activate photomorphogenesis signals (Probable). {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Short anther filaments, delayed anther dehiscence
CC and greatly reduced male fertility. Myb21 and myb24 double mutant is
CC more severely sterile than myb21 mutant and has petals that just grew
CC out of the sepals but ended at a lower level than the stigma. Myb21 and
CC myb57 double mutant has an intermediate sterility phenotype and petals
CC that grew to a final height parallel to the pistil. Myb21, myb24 and
CC myb57 triple mutant has a strongly reduced fertility and an arrested
CC growth of the petals that never grew out of the sepals.
CC {ECO:0000269|PubMed:16805732, ECO:0000269|PubMed:19325888}.
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DR EMBL; AB005888; BAA21618.1; -; mRNA.
DR EMBL; AP000371; BAB02527.1; -; Genomic_DNA.
DR EMBL; AB018114; BAB02527.1; JOINED; Genomic_DNA.
DR EMBL; CP002686; AEE77366.1; -; Genomic_DNA.
DR EMBL; AY519589; AAS10059.1; -; mRNA.
DR EMBL; AK118439; BAC43048.1; -; mRNA.
DR EMBL; AB493635; BAH30473.1; -; mRNA.
DR EMBL; AF062870; AAC83592.1; -; mRNA.
DR PIR; T51642; T51642.
DR RefSeq; NP_189418.2; NM_113696.3.
DR AlphaFoldDB; Q9LK95; -.
DR SMR; Q9LK95; -.
DR BioGRID; 7731; 19.
DR IntAct; Q9LK95; 8.
DR STRING; 3702.AT3G27810.1; -.
DR PaxDb; Q9LK95; -.
DR PRIDE; Q9LK95; -.
DR ProteomicsDB; 251329; -.
DR EnsemblPlants; AT3G27810.1; AT3G27810.1; AT3G27810.
DR GeneID; 822401; -.
DR Gramene; AT3G27810.1; AT3G27810.1; AT3G27810.
DR KEGG; ath:AT3G27810; -.
DR Araport; AT3G27810; -.
DR TAIR; locus:2089159; AT3G27810.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_028567_25_8_1; -.
DR InParanoid; Q9LK95; -.
DR OMA; NADLMGT; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; Q9LK95; -.
DR PRO; PR:Q9LK95; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LK95; baseline and differential.
DR Genevisible; Q9LK95; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:1990841; F:promoter-specific chromatin binding; IPI:TAIR.
DR GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEP:TAIR.
DR GO; GO:0009867; P:jasmonic acid mediated signaling pathway; IEP:TAIR.
DR GO; GO:0009585; P:red, far-red light phototransduction; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0009753; P:response to jasmonic acid; IEP:TAIR.
DR GO; GO:0048443; P:stamen development; IMP:TAIR.
DR GO; GO:0080086; P:stamen filament development; IGI:TAIR.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR044676; EOBI/EOBII-like_plant.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR PANTHER; PTHR45675; PTHR45675; 1.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Phytochrome signaling pathway;
KW Reference proteome; Repeat; Transcription; Transcription regulation;
KW Ubl conjugation.
FT CHAIN 1..226
FT /note="Transcription factor MYB21"
FT /id="PRO_0000197076"
FT DOMAIN 17..69
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 70..124
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 45..69
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 97..120
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 116
FT /note="W -> C (in Ref. 1; BAA21618)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 226 AA; 25351 MW; D29479EBFD1D59CC CRC64;
MEKRGGGSSG GSGSSAEAEV RKGPWTMEED LILINYIANH GDGVWNSLAK SAGLKRTGKS
CRLRWLNYLR PDVRRGNITP EEQLIIMELH AKWGNRWSKI AKHLPGRTDN EIKNFWRTRI
QKYIKQSDVT TTSSVGSHHS SEINDQAAST SSHNVFCTQD QAMETYSPTP TSYQHTNMEF
NYGNYSAAAV TATVDYPVPM TVDDQTGENY WGMDDIWSSM HLLNGN
